Desulfobulbus rhabdoformis sp. nov., a sulfate reducer from a water-oil separation system

Lien, Torleiv; Madsen, Marit Steine; Steen, Ida Helene; Gjerdevik, Kjersti

doi:10.1099/00207713-48-2-469

Cited by 69 publications

(44 citation statements)

References 26 publications

(11 reference statements)

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“…Similarly, D. rhabdoformis was isolated from a water-oil separation system (71), and the presence of this genus has been widely described in petroleum reservoirs (72,73). D. propionicus also has been shown to grow using an electrode as the sole electron acceptor (74).…”

Section: Discussionmentioning

confidence: 99%

Anodes Stimulate Anaerobic Toluene Degradation via Sulfur Cycling in Marine Sediments

Daghio

Vaiopoulou

Patil

et al. 2016

Appl Environ Microbiol

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cHydrocarbons released during oil spills are persistent in marine sediments due to the absence of suitable electron acceptors below the oxic zone. Here, we investigated an alternative bioremediation strategy to remove toluene, a model monoaromatic hydrocarbon, using a bioanode. Bioelectrochemical reactors were inoculated with sediment collected from a hydrocarbon-contaminated marine site, and anodes were polarized at 0 mV and ؉300 mV (versus an Ag/AgCl [3 M KCl] reference electrode). The degradation of toluene was directly linked to current generation of up to 301 mA m ؊2 and 431 mA m ؊2 for the bioanodes polarized at 0 mV and ؉300 mV, respectively. Peak currents decreased over time even after periodic spiking with toluene. The monitoring of sulfate concentrations during bioelectrochemical experiments suggested that sulfur metabolism was involved in toluene degradation at bioanodes. 16S rRNA gene-based Illumina sequencing of the bulk anolyte and anode samples revealed enrichment with electrocatalytically active microorganisms, toluene degraders, and sulfate-reducing microorganisms. Quantitative PCR targeting the ␣-subunit of the dissimilatory sulfite reductase (encoded by dsrA) and the ␣-subunit of the benzylsuccinate synthase (encoded by bssA) confirmed these findings. In particular, members of the family Desulfobulbaceae were enriched concomitantly with current production and toluene degradation. Based on these observations, we propose two mechanisms for bioelectrochemical toluene degradation: (i) direct electron transfer to the anode and/or (ii) sulfide-mediated electron transfer.

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Section: Discussionmentioning

confidence: 99%

Anodes Stimulate Anaerobic Toluene Degradation via Sulfur Cycling in Marine Sediments

Daghio

Vaiopoulou

Patil

et al. 2016

Appl Environ Microbiol

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“…Methanosaeta are thus particularly susceptible to competition from other acetate-utilizing MA (Jetten et al, 1990) and SRB (Ward and Winfrey, 1985). In contrast, SRB in the genus Desulfobulbus are able to couple the respiration of sulfate to the oxidation of three-carbon compounds and can also grow fermentatively, mixotrophically or by using nitrate or nitrite as an alternative electron acceptor (Stams, 1984;Lien et al, 1998;Sass et al, 2002;Suzuki et al, 2007). Because of their geochemical importance, natural ecological linkage and contrasting metabolic flexibility, Desulfobulbus and Methanosaeta represent useful model taxa to analyze microbial distribution patterns and some basic aspects of microbial ecology such as niche partitioning.…”

Section: Introductionmentioning

confidence: 99%

Contrasting patterns of niche partitioning between two anaerobic terminal oxidizers of organic matter

Oakley

Carbonero

Dowd³

et al. 2011

The ISME Journal

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Understanding the ecological principles underlying the structure and function of microbial communities remains an important goal for microbial ecology. We examined two biogeochemically important taxa, the sulfate-reducing bacterial genus, Desulfobulbus, and the methanogenic archaeal genus, Methanosaeta, to compare and contrast niche partitioning by these two taxa that are ecologically linked as anaerobic terminal oxidizers of organic material. An observational approach utilizing functional gene pyrosequencing was combined with a community-based reciprocal incubation experiment and characterization of a novel Desulfobulbus isolate. To analyze the pyrosequencing data, we constructed a data analysis pipeline, which we validated with several control data sets. For both taxa, particular genotypes were clearly associated with certain portions of an estuarine gradient, consistent with habitat or niche partitioning. Methanosaeta genotypes were generally divided between those found almost exclusively in the marine habitat (B30% of operational taxonomic units (OTUs)), and those which were ubiquitously distributed across all or most of the estuary (B70% of OTUs). In contrast to this relatively monotonic distribution, for Desulfobulbus, there were many more genotypes, and their distributions represented a wide range of inferred niche widths from specialist genotypes found only at a single site, to ubiquitous or generalist genotypes found in all 10 sites examined along the full estuarine gradient. Incubation experiments clearly showed that, for both taxa, communities from opposite ends of the estuary did not come to resemble one another, regardless of the chemical environment. Growth of a Desulfobulbus isolated into pure culture indicated that the potential niche of this organism is significantly larger than the realized niche. We concluded that niche partitioning can be an important force structuring microbial populations, with biotic and abiotic components having very different effects depending on the physiology and ecology of each taxon.

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“…fields (Beeder et al, 1995;Lien et al, 1998;MirandaTello et al, 2003), hot springs (Mori et al, 2003) and rice field soil (Ouattara et al, 1999).…”

mentioning

confidence: 99%

Diversity of substrate utilization and growth characteristics of sulfate-reducing bacteria isolated from estuarine sediment in Japan

Suzuki

Ueki

Amaishi

et al. 2007

J. Gen. Appl. Microbiol.

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Desulfobulbus rhabdoformis sp. nov., a sulfate reducer from a water-oil separation system

Cited by 69 publications

References 26 publications

Anodes Stimulate Anaerobic Toluene Degradation via Sulfur Cycling in Marine Sediments

Anodes Stimulate Anaerobic Toluene Degradation via Sulfur Cycling in Marine Sediments

Contrasting patterns of niche partitioning between two anaerobic terminal oxidizers of organic matter

Diversity of substrate utilization and growth characteristics of sulfate-reducing bacteria isolated from estuarine sediment in Japan

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