Diversity of substrate utilization and growth characteristics of sulfate-reducing bacteria isolated from estuarine sediment in Japan

Suzuki, Daisuke; Ueki, Atsuko; Amaishi, Aya; Ueki, Katsuji

doi:10.2323/jgam.53.119

Cited by 16 publications

(19 citation statements)

References 53 publications

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“…Cell motility was examined by phase-contrast microscopy (BW50WI; Olympus). Physiological tests were performed according to the methods described by Suzuki et al (2007). The optimum growth conditions of strain DS T were investigated by using the defined medium containing phenol.…”

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confidence: 99%

Reclassification of Desulfobacterium anilini as Desulfatiglans anilini comb. nov. within Desulfatiglans gen. nov., and description of a 4-chlorophenol-degrading sulfate-reducing bacterium, Desulfatiglans parachlorophenolica sp. nov.

Suzuki

Cui

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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Reclassification of Desulfobacterium anilini as Desulfatiglans anilini comb. nov. within Desulfatiglans gen. nov., and description of a 4-chlorophenol-degrading sulfate-reducing bacterium, Desulfatiglans parachlorophenolica sp. nov. Species of the genus Desulfobacterium are Gram-negative, oval to rod-shaped, or spherical, strictly anaerobic bacteria (Kuever et al., 2005). Species of the genus are widespread in brackish or marine sediment. Several sulfate-reducing bacteria have been classified as species of the genus Desulfobacterium on the basis of these phenotypic characteristics (Bak & Widdel, 1986a, b;Szewzyk & Pfennig 1987;Gogotova & Vainshtein, 1989;Schnell et al., 1989). Three recognized species of the genus Desulfobacterium were described by Kuever et al. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain DS T is AB763347.

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confidence: 99%

Reclassification of Desulfobacterium anilini as Desulfatiglans anilini comb. nov. within Desulfatiglans gen. nov., and description of a 4-chlorophenol-degrading sulfate-reducing bacterium, Desulfatiglans parachlorophenolica sp. nov.

Suzuki

Cui

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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“…nov. (Suzuki et al, 2007b). In this paper, we describe the characterization of another isolate, designated strain MSL71 T , that oxidizes butyrate as one of the electron donors for sulfate reduction (Suzuki et al, 2007c). The phylogenetic, physiological and chemotaxonomic characteristics of MSL71 T supported the proposal of a novel genus and species in the class Deltaproteobacteria for this strain.…”

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confidence: 71%

“…Sediment cores were collected to a depth of 10 cm with a core sampler (5 cm in diameter) from sediment at a water depth of 2 m in the Niida River estuary in Sakata Harbour, Japan (Suzuki et al, 2007c). The sediment sample was diluted by consecutive 10-fold dilutions with sterilized seawater bubbled with O 2 -free N 2 gas.…”

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confidence: 99%

“…Several strains of SRB were obtained by picking up black colonies of SRB that appeared on the roll-tube agar after incubation for about 1 month. Strain MSL71 T was finally obtained after several purification procedures through colony isolation using the anaerobic roll-tube method (Suzuki et al, 2007c (Widdel et al, 1983) and 0.5 g L-cysteine . HCl .…”

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confidence: 99%

“…Thus, strain MSL71 T is valuable as a novel SRB isolated from marine environments that shows incomplete oxidization of butyrate. Furthermore, it is noteworthy that, although almost all known butyrateoxidizing SRB species have been isolated by using enrichment cultures with various electron donors (Kuever et al, 2005), strain MSL71 T was isolated directly by picking up a colony using the agar roll-tube method with lactate as an electron donor (Suzuki et al, 2007c).…”

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Desulfoluna butyratoxydans gen. nov., sp. nov., a novel Gram-negative, butyrate-oxidizing, sulfate-reducing bacterium isolated from an estuarine sediment in Japan

Suzuki

Ueki

Amaishi

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Desulfoluna butyratoxydans gen. nov., sp. nov., a novel Gram-negative, butyrate-oxidizing, sulfatereducing bacterium isolated from an estuarine sediment in Japan Daisuke Suzuki, Atsuko Ueki, Aya Amaishi3 and Katsuji Ueki Correspondence Atsuko Ueki uatsuko@tds1.tr.yamagata-u.ac.jp Faculty of Agriculture, Yamagata University, Wakaba-machi 1-23, Tsuruoka, Yamagata 997-8555, Japan A strictly anaerobic, mesophilic, sulfate-reducing bacterial strain, designated MSL71 T , was isolated from an estuarine sediment from the Sea of Japan bordering the Japanese islands and was characterized phenotypically and phylogenetically. The cells were found to be Gram-negative, motile, non-spore-forming, slightly curved rods. Catalase and oxidase activities were not detected. The optimum NaCl concentration for growth was 2.0 % (w/v), the optimum temperature was 30 6C and the optimum pH was 6.3. Strain MSL71 T utilized formate, butyrate, pyruvate, lactate, malate, ethanol, propanol, butanol, glycerol and H 2 as electron donors for sulfate reduction. The organic electron donors used were incompletely oxidized, mainly to acetate. The strain did not use acetate, propionate, fumarate, succinate, methanol, glycine, alanine, serine, aspartate or glutamate. Sulfite and thiosulfate were used as electron acceptors with lactate as an electron donor, but fumarate was not utilized. Without electron acceptors, pyruvate and malate, but not lactate or fumarate, were fermented. The genomic DNA G+C content was 62.0 mol%. Menaquinone MK-8(H 4 ) was the major respiratory quinone. The major cellular fatty acids were C 14 : 0 , C 16 : 0 , C 16 : 1 v7, C 18 : 1 v9, C 18 : 1 v7 and C 14 : 0 3-OH. A phylogenetic analysis based on the 16S rRNA gene sequence placed the strain in the class Deltaproteobacteria. The closest recognized relative of strain MSL71 T was Desulfofrigus fragile (93.9 % sequence similarity) and the next closest recognized species was Desulfofrigus oceanense (93.5 %). On the basis of the significant differences in the 16S rRNA gene sequence and phenotypic characteristics between strain MSL71 T and each of the related species, a novel genus and species, Desulfoluna butyratoxydans gen. nov., sp. nov., are proposed to accommodate strain MSL71 T . The type strain is MSL71 T (5JCM 14721 T 5DSM 19427 T ).Sulfate-reducing bacteria (SRB) are capable of utilizing various compounds, such as H 2 , fatty acids, alcohols, amino acids and sugars, as well as aliphatic and aromatic hydrocarbons, as electron donors for sulfate reduction (Hansen, 1993;Castro et al., 2000;Rabus et al., 2000). Major intermediates of anaerobic decomposition of organic matter, such as formate, acetate, propionate, butyrate, lactate and H 2 , serve as the most important electron donors for sulfate reduction, and thus SRB contribute significantly to the mineralization of organic matter and the sulfur cycle (Sørensen et al., 1981;Jørgensen, 1982). In the course of an investigation of SRB in an estuarine sediment from the Japanese islands, we isolated various strains of phylogenetic...

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Desulfogranum gen. nov .

Galushko

Kuever

2020

Bergey's Manual of Systematics of Archaea and Bacteria

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De.sul.fo.gra'num. L. pref. de , from; L. neut. n. sulfur , sulfur; N.L. pref. desulfo ‐, prefix used to characterize a dissimilatory sulfate‐reducing prokaryote; L. neut. n. granum , grain; N.L. neut. n. Desulfogranum , a sulfate‐reducing grain. Desulfobacterota / Desulfobulbia / Desulfobulbales / Desulfobulbaceae / Desulfogranum gen. nov. Oval‐shaped rods, 0.8–1.7 × 1.0–3.2 μm. Occur singly or in pairs. Spore formation is not observed. Gram‐stain‐negative. Motility might occur depending on the species. Anaerobic, having a respiratory and fermentative type of metabolism. Chemoorganoheterotrophic, using propionate, ethanol, propanol, lactate, and pyruvate as carbon sources and electron donors. Some species can use mono‐ and disaccharides. Electron donors are incompletely oxidized to acetate. For growth on H 2 + CO 2 (chemolithoheterotrophic growth) and formate, yeast extract or acetate is required. Sulfate and thiosulfate serve as terminal electron acceptors and are reduced to sulfide; sulfite is used by some species. Fermentative growth on pyruvate (all species) and sugars (some species). Mesophilic, with an optimal growth temperature between 25 and 35°C. Neutrophilic, with optimal pH range 6.7–7.5. All species show optimal growth in media with 20–30 g/l of NaCl. Media containing a reductant are necessary for growth. Vitamins or yeast extract seems to be required for growth of some species. Three former members of the genus Desulfobulbus are here reclassified as a new genus Desulfogranum within the family Desulfobulbaceae . Desulfogranum species occur in anoxic marine and brackish water sediments. DNA G + C content (mol%) : 45.8–57.5 (genome). Type species : Desulfogranum mediterraneum comb. nov. (basonym: Desulfobulbus mediterraneus Sass et al. 2002, VL87).

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Diversity of substrate utilization and growth characteristics of sulfate-reducing bacteria isolated from estuarine sediment in Japan

Cited by 16 publications

References 53 publications

Reclassification of Desulfobacterium anilini as Desulfatiglans anilini comb. nov. within Desulfatiglans gen. nov., and description of a 4-chlorophenol-degrading sulfate-reducing bacterium, Desulfatiglans parachlorophenolica sp. nov.

Reclassification of Desulfobacterium anilini as Desulfatiglans anilini comb. nov. within Desulfatiglans gen. nov., and description of a 4-chlorophenol-degrading sulfate-reducing bacterium, Desulfatiglans parachlorophenolica sp. nov.

Desulfoluna butyratoxydans gen. nov., sp. nov., a novel Gram-negative, butyrate-oxidizing, sulfate-reducing bacterium isolated from an estuarine sediment in Japan

Desulfogranum gen. nov .

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