Desoxyribose nucleic acid in embryonic diploid and haploid tissues

Moore, Betty C.

doi:10.1007/bf00325791

Cited by 29 publications

(3 citation statements)

References 19 publications

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“…Since then a large literature has appeared on this subject (Davidson, 1952). It is hardly to be expected that exactly constant figures for the various species would be demonstrated, and some recent papers draw attention to the wide spread of values found, for example, in the cells of embryonic tissues, the range being greater for the younger cell types (Moore, 1952). A log-normal distribution of the average DNAP per cell for a variety of normal and pathological bone-marrow samples has been demonstrated in our results.…”

Section: Discussionsupporting

confidence: 57%

Nucleic acids of bone marrow cells, with special reference to pernicious anæmia

White¹,

Leslie²,

Davidson³

1953

J. Pathol.

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IN earlier work on the nucleic acids of bone marrow cells (Davidson, Leslie and White, 1951a and b ) , the amount of deoxyribonucleic acid phosphorus (DNAP) per cell was found to vary within narrow limits in health and in a variety of blood disorders, including leukaemias, but the ribonucleic acid phosphorus was more variable, and was related to the developmental stage of the cells. In untreated pernicious anaemia, however, both DNAP and RNAP were excessively abundant per cell, and only the RNAP declined rapidly towards normal when the anzmia was treated specifically. In view of the apparent close connection between the nucleic acids and normal cellular growth and differentiation (Brachet, 1947 ; Caspersson, 1950 ;Davidson, 1950), the study of these substances is an important aspect of quantitative cellular pathology. The present work extends our previous observations and subjects the results t o a more searching statistical analysis. Spermatozoa are also included among the cells examined. TECHNIQUEThe methods for obtaining aspirated sternal marrow samples and blood leucocytes for cell examination and for estimation of nucleic acids have been previously described White, 1948, 1951a and b).Several methods used for the examination of reticulocytes are described under Results.The sperms in fresh seminal samples were freed from plasma by centrifuging three times with 0.85 per cent. sodium chloride solution containing 0.001M sodium citrate, and then suspended in a further 2 ml. of saline solution. 0.5 ml. of the suspension was withdrawn and diluted 1 in 20 with 1 per cent. formalin in 0.85 per cent. sodium chloride for counting in the improved Neubauer hzemocytometer chamber, under the phase-contrast microscope. The remainder of the suspension was used for analysis of nucleic acids. The seminal samples had been investigated for evidence of male infertility by Dr G. E. Wachtel of the Institute of Obstetrics and Gynaxology, University of London, and all those included in the results exhibited no deviation from normal. 291 Retieulocyta. Spermatozoa. Blood, vi, 344. Suppl. 1, 101. New York. p. 357. J . Biol. Chem., clxi, 83. Ibid., clxi, 293. Cold Spring Harbor Symp. Quant. J. Biol. Chem., clsxiii, 799. Biol., xiii, 185. Biochem. J., xlix, xv. Physiol. Zool., xxiii, 169. Studies on the formation of cellular substances during blood cell production, London, p. 104 ff. Blood, v, 695.Biochem. J., xlvi, xsiv.

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Section: Discussionsupporting

confidence: 57%

Nucleic acids of bone marrow cells, with special reference to pernicious anæmia

White¹,

Leslie²,

Davidson³

1953

J. Pathol.

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“…Progressive differentiation may also be associated with quantitative differences in DNA amounts in amphibians. Moore (1952), in working with haploid and diploid tissues of Rana pipiens embryos, found that the range of DNA values in the forebrain was greater in the 7-day embryos than in the 11 -day embryos. She found no correlation between mitotic activity and the amount of DNA.…”

Section: Discussionmentioning

confidence: 99%

“…Also it has been shown in certain molds that both cell size and nuclear size changes may accompany morphogenesis (Bonner, 1957). Furthermore, it has been hypothesized that DNA may show a slight decrease with the progressive differentiation of certain R. pipiens tadpole tissues (Moore. 1952).…”

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confidence: 99%

Nuclear Sizes in Rana Mesonephroi

Connell

1959

The Biological Bulletin

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When Jacobj (1925) devised the now classical caryometric method there was opened up for the cytologist a whole new field in population dynamics. Jacobj demonstrated that for any given organ the nuclear population showed considerable variation in size from one individual nucleus to another. By plotting frequency distribution curves of nuclear volumes he obtained evidence to show that the volumes increased discontinuously according to a logical pattern. The peaks of the curve corresponded to nuclear volumes which when arranged in series gave the geometric progression 1 : 2 : 4 : 8. Nuclear class series have since been described for a wide range of both invertebrate and vertebrate tissues.Caryometry has been used extensively in investigations of ploidy, of endomitotic growth, and of the interphasic growth of nuclei in a dividing tissue. The concept

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