The Chemistry of the Cell Nucleus

Allfrey, Vincent G.; Mirsky, A. E.; Stern, Herbert

doi:10.1002/9780470122617.ch8

Cited by 38 publications

(3 citation statements)

References 218 publications

(23 reference statements)

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“…Table 1 shows that high enzyme activities occur in the liver, kidney and the lung and to a lesser extent in several other tissues. This regional distribution is similar to the distribution previously demonstrated for esterases (Allfrey et al 1955; Moroi & Kuga 1980).…”

Section: Methodssupporting

confidence: 90%

Peripheral Metabolism of β‐Carboline‐Carboxylic Acid Esters

Simonsen

Nielsen

Bræstrup

1982

Acta Pharmacologica et Toxicologica

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Absrract: Esters of P-carboline-3-carboxylic acid have recently been identified as potent inhibitors of brain benzodiazepine receptors in vitro. Ethyl ~-carboline-3-carboxylate (P-CCE), however, is a rather weak inhibitor in vivo of benzodiazepine receptors in mice. The EDSO-value was 91 mg/kg intraperitoneally 35 min. after administration (ED50 is that dose which inhibits by 50% the specific binding of 3H-flunitrazepam intravenously). ED50 for P-CCE was 2-20 fold lower in mice pretreated with organophosphorus esterase inhibitors, concomitantly with the observation of strong inhibition of liver and kidney hydrolyzing activity, using 'Hpropyl P-carboline-3-carboxylate as substrate. The rat brain contains only approximately 0.1 % of the hydrolyzing activity as compared to the liver. It is concluded that some esters of P-carboline-3-carboxylate exhibit only weak effects on benzodiazepine receptors in living animals due to hydrolysis outside the brain.

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Section: Methodssupporting

confidence: 90%

Peripheral Metabolism of β‐Carboline‐Carboxylic Acid Esters

Simonsen

Nielsen

Bræstrup

1982

Acta Pharmacologica et Toxicologica

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“…These systematic in vitro studies have unveiled the disparate mechanisms involved in TFs/DNA interactions with DNA methylation being a key leverage in this process. However, it has been shown that only a small fraction of TF binding sites (TFBS) found in vitro can be validated in vivo (10), due to a more complex and crowded nuclear environment involving the chromatin structure and non-DNA molecules such as RNAs, histones and non-histone proteins (11). Therefore, further efforts are required to characterise the methylation status of TFBS in the cell context.…”

Section: Introductionmentioning

confidence: 99%

MethMotif: an integrative cell specific database of transcription factor binding motifs coupled with DNA methylation profiles

Lin

Sian

et al. 2018

Nucleic Acids Research

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Several recent studies have portrayed DNA methylation as a new player in the recruitment of transcription factors (TF) within chromatin, highlighting a need to connect TF binding sites (TFBS) with their respective DNA methylation profiles. However, current TFBS databases are restricted to DNA binding motif sequences. Here, we present MethMotif, a two-dimensional TFBS database that records TFBS position weight matrices along with cell type specific CpG methylation information computed from a combination of ChIP-seq and whole genome bisulfite sequencing datasets. Integrating TFBS motifs with TFBS DNA methylation better portrays the features of DNA loci recognised by TFs. In particular, we found that DNA methylation patterns within TFBS can be cell specific (e.g. MAFF). Furthermore, for a given TF, different DNA methylation profiles are associated with different DNA binding motifs (e.g. REST). To date, MethMotif database records over 500 TFBSs computed from over 2000 ChIP-seq datasets in 11 different cell types. MethMotif portal is accessible through an open source web interface (https://bioinfo-csi.nus.edu.sg/methmotif) that allows users to intuitively explore the entire dataset and perform both single, and batch queries.

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“…From results of much work, it may be concluded that D N A concentration in the liver and other organs and tissues is relatively stable (BOIVIN et al 1948;VENDRELY and VENDRELY 1949;ALLFREY et al 1955). RNA concentration, on the other hand, reflects the changes in the physiological, but especially in the metabolic processes which are developed as a result of the action of various genetic and epigenetic factors (ENESCO and LEBLOND 1962;LEPORE et al 1965;WEHLING et al 1972;GIOVANNETTI and STOTHERS 1975).…”

Section: Introductionmentioning

confidence: 99%