Desorption chemical ionization mass spectrometry of guanidino compounds

Esmans, Eddy L.; Alderweireldt, F. C.; Marescau, B.; Löwenthal, A.

doi:10.1021/ac00268a024

Cited by 7 publications

(3 citation statements)

References 10 publications

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“…So far only Na-acetylated amino acids are well known compounds in biochemical reactions and have specific functions in metabolism; the most frequently observed acetylated residues are amino acids other than arginine (44). NOL acetylated arginine can be detected in a rare hereditary disorder of the urea cycle, which causes hyperargininemia (45).…”

Section: Discussionmentioning

confidence: 99%

15 N Investigation into the Effect of a Pollutant on the Nitrogen Metabolism of Tetrahymena pyriformis as a Model for Environmental Medical Research

Arndt¹,

Hofmann²,

Gehre³

et al. 1998

Environmental Health Perspectives

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A pilot study was perfonned to examine the potential of stable isotope tiques for monitoring the impact of a harmfil substance on the cellular nitrogen metabolism in the ciliate species Tenra/mnayfornnis. After identical cultivation periods ofcontrol cals and toluene-exposed cells in a defined culture medium enriched with (guanidino5N2]L-argnine, a number of nitrogen-contaning pools were analyzech 1) quantity and 15N abundance of ammonia as the end product of nitrogen metabolism in the sytem; 2) pattem and 15N abundances of the protein-bound amino acids in thecells; 3) patten and 15N abundancesoffreeamino acids inthe clls; and 4) pattern and 15N abundances of the amino acids in the culture medium. In addition to 15N emission spectometry, a new gas chromatogrhybcombuston interace-iotope ratio mass spectrometry/mass spectrometry an cal stem was used. The production and 15N content of ammonia were higher in the toluene-exposed sysem by 30% and 43%, respectily, indig higher demination rates and greater argnie consumptont The toluene-exposed cells exhibited increased 15N abundances ofprotein-bound amino acids in alanine, aspartic acid, gluhtmic acid, and tyrosine. Furthermore, strctural anals revealed the presence of N0-acelarginine and pyrrolidonecarboxylic acid-compounds dtat had not previously been detcted in Tena4ym aprfni. Differences in the '5N-enrichment of fee amino acids were also evident. This new effect-monitoring stem designed to investigte the impact ofa pollut on protein metabolism by using a stable isotope-labeled cell cuture is a powerfil tool for environmentl medical rsach. Key worux amino acids, GC-C-IRMS, GC-MS, nitro- Cell culture has been used frequently as a test system for toxicity assessment in pharmacology (4,6,7) and ecotoxicology (8,9). By observing end points such as growth impairment (10), modification of motility (7,11), and inner and outer morphology (6,12,13)

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Section: Discussionmentioning

confidence: 99%

15 N Investigation into the Effect of a Pollutant on the Nitrogen Metabolism of Tetrahymena pyriformis as a Model for Environmental Medical Research

Arndt¹,

Hofmann²,

Gehre³

et al. 1998

Environmental Health Perspectives

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

15N investigation into the effect of a pollutant on the nitrogen metabolism of Tetrahymena pyriformis as a model for environmental medical research.

Arndt

Hofmann

Gehre

et al. 1998

Environ Health Perspect

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A pilot study was performed to examine the potential of stable isotope techniques for monitoring the impact of a harmful substance on the cellular nitrogen metabolism in the ciliate species Tetrahymena pyriformis. After identical cultivation periods of control cells and toluene-exposed cells in a defined culture medium enriched with [guanidino-15N2]l-arginine, a number of nitrogen-containing pools were analyzed: 1) quantity and 15N abundance of ammonia as the end product of nitrogen metabolism in the system; 2) pattern and 15N abundances of the protein-bound amino acids in the cells; 3) pattern and 15N abundances of free amino acids in the cells; and 4) pattern and 15N abundances of the amino acids in the culture medium. In addition to 15N emission spectrometry, a new gas chromatography/combustion interface-isotope ratio mass spectrometry/mass spectrometry analytical system was used. The production and 15N content of ammonia were higher in the toluene-exposed system by 30% and 43%, respectively, indicating higher deamination rates and greater arginine consumption. The toluene-exposed cells exhibited increased 15N abundances of protein-bound amino acids in alanine, aspartic acid, glutamic acid, and tyrosine. Furthermore, structural analyses revealed the presence of N[Omega]-acetylarginine and pyrrolidonecarboxylic acid--compounds that had not previously been detected in Tetrahymena pyriformis. Differences in the 15N-enrichment of free amino acids were also evident. This new effect-monitoring system designed to investigate the impact of a pollutant on protein metabolism by using a stable isotope-labeled cell culture is a powerful tool for environmental medical research.

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“…Since they are sodium salts, a MS method with the desorption type ion source should be employed. Such instruments include, for example, one with the ion sources of field desorption (FD) [4], secondary ion mass spectrometry (SIMS) [5], those with desorption chemical ionization (DCI) [6], laser desorption/ionization (LDI) [7], fast atom bombardment (FAB) [8], and some other types [9] [10]. For the analysis of low-and non-volatile organic, bioorganic, and organoelement compound, however, the most applied mass spectrometers are now the matrix assisted laser desorption/ionization (MALDI), and electrospray ionization (ESI) instruments [11] [12] [13] [14] [15].…”

Section: Introductionmentioning

confidence: 99%

Reactivity of N-Methylidenemalonates of 3-Arylaminoindoles and p-Dimethylamino-N-Phenylaniline in the Course of Their Analysis by Electrospray Ionization Mass Spectrometry

Nekrasov¹,

Ikonnikov²,

Borisov³

et al. 2017

IJAMSC

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The behavior of N-methylidenemalonates of 3-arylaminoindoles and p-dimetylamino-N-phenylanyline (M = ANa) was studied during their analysis with ESI mass spectrometer operated in negative (NI) ions included elimination of two water molecules that was supported by their MS 2 spectra. A common feature of the NI-and PI-ESI mass spectra was the presence of oligomeric ions, up to tetramers and trimers for the NI-and PI-ESI ones, respectively. The oligomers were formed by interaction of the corresponding ions with neutral molecules. When ions contained extra hydrogen atoms, they were introduced by hydrolysis.

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Desorption chemical ionization mass spectrometry of guanidino compounds

Cited by 7 publications

References 10 publications

15 N Investigation into the Effect of a Pollutant on the Nitrogen Metabolism of Tetrahymena pyriformis as a Model for Environmental Medical Research

15 N Investigation into the Effect of a Pollutant on the Nitrogen Metabolism of Tetrahymena pyriformis as a Model for Environmental Medical Research

15N investigation into the effect of a pollutant on the nitrogen metabolism of Tetrahymena pyriformis as a model for environmental medical research.

Reactivity of N-Methylidenemalonates of 3-Arylaminoindoles and p-Dimethylamino-N-Phenylaniline in the Course of Their Analysis by Electrospray Ionization Mass Spectrometry

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