Desmopressin stimulates bile secretion in anesthetized rats

Ghazaee, Sepideh Parchami; Gorenko, Zoya; Karbovska, Ludmila S; Veselsky, S.P.; Yanchuk, Petro I.; Makarchuk, Mykola Yukhymovych

doi:10.4149/gpb_2010_02_151

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…Previously we showed that corvitin significantly increased blood flow in rat liver [13], that improves hepatic tissue respiration and provides a better energy supply of synthetic and metabolic processes. It is suggested that QUE is accumulated in the mitochondria increasing mitochondrial biogenesis [28][29][30] and improving mitochondrial bioenergetics [31].…”

Section: Animal Groupmentioning

confidence: 89%

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The features of bile acids exchange in rats under the influence of corvitin

Vovkun¹,

Yanchuk²,

Shtanova³

et al. 2017

Ukr.Biochem.J.

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Corvitin is a soluble form of quercetin (QUE) and its effects are based on the ability to inhibit the activity of 5-lipoxygenase and to block the formation of leukotrienes. Corvitin increases bloodflow inE nzyme 5-lipoxygenase (5-LO) plays an essential role in the biosynthesis of leukotrienes (LTs). Leukotriene LTB4 is a potent chemotactic and chemokinetic agent for a variety of leukocytes, while cysteinyl leukotrienes C4, D4 and E4 regulate vascular permeability and smooth muscle contraction. These compounds have vasoconstrictor properties and cause prolonged increased pressure in large blood vessels and capillaries. Inhibitors of LT synthesis have been hypothesized to possess therapeutic potential for the treatment of asthma, allergic disorders and other diseases [1]. Physiological effects of LTs on the liver are not stu died enough. It was shown that the addition of LTD4 to liver perfusate resulted in a decrease of portal blood flow, bile flow and bile acids (BAs) release [2,3]. Specific blockers of 5LO activity do not affect the synthesis of other arachidonic acid metabolites -prostaglandins and thromboxane [4].Formation of bile by hepatocytes is an important way of removing potentially harmful exogenous © 2017 Vovkun T. V. et al. This is an openaccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. lipophilic substances and endogenous compounds such as bilirubin and cholesterol [5]. Bile contains ВAs, which are synthesized in the liver from cholesterol. These molecules ensure the stability of bile colloidal system, dispersion and absorption of dietary fats in the intestine, activation of pancreatic lipase, intensification of intestinal motility [6].Plant polyphenol quercetin (QUE) attracts attention due to a wide range of positive effects on human health including hepatoprotective effect [7]. But its impact on bile formation and secretion has not been studied. It is known that QUE effects depend on applied doses, redox status of target cells and metabolites synthesis [8]. Despite the high effectiveness of QUE its bioavailability is very low. This complicates the investigation of QUE effects in vivo. In this study we used a water-soluble form of QUE corvitin at different doses. Corvitin is the inhibitor of 5LO, it is natural compound without side effects, that has been used to treat cardiac disorders [9]. Previously we found that this flavonoid modulates gastric sedoi: https://doi.org/10.15407/ubj89.05.106

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Section: Animal Groupmentioning

confidence: 89%

“…Free and conjugated BAs were separated by thin layer chromatography method that has been described by Veselskiy et al [13]. For this purpose, 0.1 ml of bile was added to 1.9 ml of cold ethanol-acetone (1 : 3) mixture.…”

Section: Thismentioning

confidence: 99%

The features of bile acids exchange in rats under the influence of corvitin

Vovkun¹,

Yanchuk²,

Shtanova³

et al. 2017

Ukr.Biochem.J.

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“…All administra tions were performed after an equilibration period of30mintostabilizethebileflowrate.Secretedbile was collected every 30 min in the course of 2.5hour experiment by micropipette connected to a cannula, located in the bile duct. Bile lipids were separated by the method of thinlayer chromatography [16]. For this purpose, 0.1 ml of bile was added to 2.4 µl mix ture of chloroformmethanol (2 : 1), and 0.5 ml acidi fiedwater(0.5mlofconcentratedsulfuricacidper 1 liter of doubledistilled water) was added in 5 min.…”

Section: Methodsmentioning

confidence: 99%

Corvitin modulates the content of lipids in rat liver bile

Vovkun¹,

Yanchuk²,

Shtanova³

et al. 2019

Ukr.Biochem.J

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Quercetin (QUE) and its water-soluble form сorvitin are medicinally important members of the flavonoid family and the most prominent dietary antioxidants. Numerous pharmacological effects of quercetin include protection against diseases, such as atherosclerosis, myocardial infarction, and cerebrovascular disea ses. Corvitin modulates liver blood flow but its effects on liver lipid metabolism have not been understood. We investigated the influence of corvitin (2.5, 5, 10 mg/kg) on the formation and secretion of cholesterol (Chol), cholesterol esters (EChol), phospholipids (PLs), free fatty acids (FFAs), and triglycerides (TGs) into the bile. Secreted bile was collected during 2.5 h of the experiment. Lipid fractions of the bile were separated by thin-layer chromatography. We defined that administration of corvitin caused a significant (P < 0.05-0.001) increase in levels of all studied lipid components of bile. At a dose of 2.5 and 5 mg/kg corvitin increased bile content of Chol, PLs and FFAs,and at a dose of 10 mg/kg had the largest effect on the total production of EChol and TGs. We concluded that corvitin activates the liver lipids metabolism and processes of bile formation.

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Bile Synthesis Peculiarities following Changes in the Functional State of the Endothelin Receptors

Veselska¹,

Ghazaee²,

Yanchuk³

et al. 2014

JJBS

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Endothelin-1(ET-1) regulates a variety of biochemical processes in liver. However, there is no clear view concerning endothelin receptors participation in the regulation of qualitative and quantitative characteristics of liver secretory function. The purpose of this study is to evaluate the choleretic effect of ET-1 and to determine the role of ЕТ А receptors functional state in mediating the effect of ET-1 on bile and its organic components secretion. Endothelin-1 and BQ-123 (cyclo-Asppro-Val-Leu-Trp) were intraportally injected. Bile flow, bile acid concentration and content, hydroxylation and conjugation coefficients were estimated. The results of this study showed that the secreted bile volume was decreased under the effect of endothelin-1and BQ-123, although this decrease was more prolonged and profound in BQ-123 treated animals. Concentration of taurocholates, glycocholic acid and free bile acids was increased in the endothelin treated rats. When BQ-123 was administered, an increase in glycochenodeoxycholic acid+glycodeoxycholic acid (GCDCA+GDCA) and the taurin-conjugated bile acids concentration was found, whereas the free bile acids concentration altered reversely. Coefficient of hydroxylation was diminished when entholelin receptors were blocked. Activation of endothelin receptors by exogenous endothelin-1 intensified bile acids biosynthesis via "neutral pathway,"involving microsomal oxidation enzymes. The present study concludes that the endothelin receptors blockade eliminated the regulatory function of endogenous endothelin and caused a shift in bile acids synthesis to mitochondrial enzymes through "acidic pathway".

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Desmopressin stimulates bile secretion in anesthetized rats

Cited by 4 publications

References 31 publications

The features of bile acids exchange in rats under the influence of corvitin

The features of bile acids exchange in rats under the influence of corvitin

Corvitin modulates the content of lipids in rat liver bile

Bile Synthesis Peculiarities following Changes in the Functional State of the Endothelin Receptors

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