Desmoplakin is required for epidermal integrity and morphogenesis in the Xenopus laevis embryo

Bharathan, Navaneetha Krishnan; Dickinson, Amanda J.G.

doi:10.1016/j.ydbio.2019.03.010

Cited by 19 publications

(15 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Desmoplakin is a unique and critical component of desmosomal cell-cell junctions and involved in integrity of the cytoskeletal intermediate filament network (Bendrick et al, 2019). It has been shown to be required for epidermal integrity and embryo morphogenesis (Bharathan and Dickinson, 2019), as well as in the coordination of cell migration (Bendrick et al, 2019). Mutations in desmoplakin have been linked to multiple allergies, severe dermatitis and metabolic wasting (SAM) syndrome (Liang et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Deiminated proteins and extracellular vesicles - Novel serum biomarkers in whales and orca

Magnadóttir

Uysal-Onganer

Kraev

et al. 2020

Comparative Biochemistry and Physiology Part D: Genomics and Pr

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Deiminated proteins and extracellular vesicles - Novel serum biomarkers in whales and orca

Magnadóttir

Uysal-Onganer

Kraev

et al. 2020

Comparative Biochemistry and Physiology Part D: Genomics and Pr

View full text Add to dashboard Cite

show abstract

“…Desmoplakin is a unique and critical component of desmosomal cell-cell junctions and involved in integrity of the cytoskeletal intermediate filament network (Bendrick et al, 2019). It has been shown to be required for epidermal integrity and morphogenesis in the Xenopus laevis embryo (Bharathan and Dickinson, 2019) and novel roles in coordination of cell migration were recently established (Bendrick et al, 2019). Mutations in desmoplakin have been linked to multiple allergies, severe dermatitis and metabolic wasting (SAM) syndrome (Liang et al, 2019).…”

mentioning

confidence: 99%

Deiminated proteins in extracellular vesicles and serum of llama (Lama glama)—Novel insights into camelid immunity

Criscitiello

Kraev

Lange

2020

Molecular Immunology

View full text Add to dashboard Cite

A B S T R A C TPeptidylarginine deiminases (PADs) are phylogenetically conserved calcium-dependent enzymes which posttranslationally convert arginine into citrulline in target proteins in an irreversible manner, causing functional and structural changes in target proteins. Protein deimination causes generation of neo-epitopes, affects gene regulation and also allows for protein moonlighting. Furthermore, PADs have been found to be a phylogenetically conserved regulator for extracellular vesicle (EVs) release. EVs are found in most body fluids and participate in cellular communication via transfer of cargo proteins and genetic material. In this study, post-translationally deiminated proteins in serum and serum-EVs are described for the first time in camelids, using the llama (Lama glama L. 1758) as a model animal. We report a poly-dispersed population of llama serum EVs, positive for phylogenetically conserved EV-specific markers and characterised by TEM. In serum, 103 deiminated proteins were overall identified, including key immune and metabolic mediators including complement components, immunoglobulin-based nanobodies, adiponectin and heat shock proteins. In serum, 60 deiminated proteins were identified that were not in EVs, and 25 deiminated proteins were found to be unique to EVs, with 43 shared deiminated protein hits between both serum and EVs. Deiminated histone H3, a marker of neutrophil extracellular trap formation, was also detected in llama serum. PAD homologues were identified in llama serum by Western blotting, via cross reaction with human PAD antibodies, and detected at an expected 70 kDa size. This is the first report of deiminated proteins in serum and EVs of a camelid species, highlighting a hitherto unrecognized post-translational modification in key immune and metabolic proteins in camelids, which may be translatable to and inform a range of human metabolic and inflammatory pathologies.

show abstract

“…Others merely monitored the breakdown of Sanger traces from directly amplified DNA at the target site, combined with a deconvolution algorithm to predict likely repairs [20,21]. Indels that cause reading frame-shifts are a frequent outcome, so analyses with basic DNA genotyping have instead emphasized the depletion of the targeted protein measured with specific antibody staining [21][22][23]. Reported mutation efficiencies have varied, which probably reflects differing Cas9-gRNA reagent activity.…”

Section: Published Xenopus Crispr/cas9 Research -Can the Mosaicism That Results From Repair Of Cas9 Dsbs Be Eliminated?mentioning

confidence: 99%

How to increase precision in Xenopus gene editing and base editing experiments

Smith¹

2021

Preprint

View full text Add to dashboard Cite

show abstract

Desmoplakin is required for epidermal integrity and morphogenesis in the Xenopus laevis embryo

Cited by 19 publications

References 74 publications

Deiminated proteins and extracellular vesicles - Novel serum biomarkers in whales and orca

Deiminated proteins and extracellular vesicles - Novel serum biomarkers in whales and orca

Deiminated proteins in extracellular vesicles and serum of llama (Lama glama)—Novel insights into camelid immunity

How to increase precision in Xenopus gene editing and base editing experiments

Contact Info

Product

Resources

About