Desmin distribution in the cardiac outflow tract of the chick embryo during aortico-pulmonary septation.

Sumida, Hiroshi; Nakamura, Harukazu; Akimoto, Naotaka; Okamoto, Naomasa; Satow, Yukio

doi:10.1679/aohc.50.525

Cited by 14 publications

(13 citation statements)

References 16 publications

(10 reference statements)

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“…Vascular smooth muscle cells express NGF, NT-3, BDNF, NT-4/5, p75, Trk-A, Trk-B, and Trk-C. 7 Smooth muscle cells in the aorticopulmonary septum, supposed to originate from the neural crest, express ␣-SMA 61 and desmin. 62 The survival and adequate migration of these cells depends on NT-3. 63 Stromal smooth muscle cells in the prostate express NGF, BDNF and Trk-C. 6 Panniculus carnosus and arrector pili muscles in skin are strongly NT-3 positive.…”

Section: Discussionmentioning

confidence: 99%

Human and rat hepatic stellate cells express neurotrophins and neurotrophin receptors

Cassiman¹,

Denef²,

et al. 2001

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Section: Discussionmentioning

confidence: 99%

Human and rat hepatic stellate cells express neurotrophins and neurotrophin receptors

Cassiman¹,

Denef²,

et al. 2001

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“…49 Expression of so-called mesenchymal intermediate filaments (vimentin, desmin, ␣-SMA) appears not to be contradictory to the suggested neural/neuroendocrine origin, because, for example, in the aorticopulmonary septum, smooth muscle cells supposed to originate from the neural crest also express ␣-SMA 50 and desmin. 51 In view of the evidence mentioned above, unavoidable questions arise about the mesenchymal origin and functional differentiation of hepatic stellate cells. As a consequence, new studies focusing on the expression of neural and neuroendocrine markers in embryonic as well as adult liver become necessary.…”

Section: Discussionmentioning

confidence: 99%

Synaptophysin: A Novel Marker for Human and Rat Hepatic Stellate Cells

Cassiman¹,

Pelt²,

Vos³

et al. 1999

The American Journal of Pathology

141

101

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Synaptophysin is a protein involved in neurotransmitter exocytosis and is a neuroendocrine marker.We studied synaptophysin immunohistochemical expression in 35 human liver specimens (normal and different pathological conditions) , in rat models of galactosamine hepatitis and carbon tetrachloride-induced cirrhosis , and in freshly isolated rat stellate cells. Synaptophysin reactivity was present in perisinusoidal stellate cells in both human and rat normal liver biopsies. The number of synaptophysin-reactive perisinusoidal cells increased in pathological conditions. Double staining for ␣-smooth muscle actin and synaptophysin , detected by confocal laser scanning microscopy , unequivocally demonstrated colocalization of both markers in lobular stellate cells. In addition , freshly isolated rat stellate cells expressed synaptophysin mRNA (detected by polymerase chain reaction) and protein. Finally , electron microscopy showed the presence of small electron translucent vesicles , comparable to the synaptophysin-reactive synaptic vesicles in neurons , in stellate cell projections. We conclude that synaptophysin is a novel marker for quiescent as well as activated hepatic stellate cells. Together with the stellate cell's expression of neural cell adhesion molecule , glial fibrillary acidic protein , and nestin , this finding raises questions about its embryonic origin and its differentiation. In addition , the presence of synaptic vesicles in stellate cell processes suggests a hitherto unknown mechanism of interaction with neighboring cells. (Am J Pathol 1999, 155:1831-1839)

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“…An earlier study by Sumida et al (1987) had reported that the intermediate filament desmin was also found in the AP septum of the chicken embryo. Therefore the distribution of desmin was compared in the present study with the distribution of smooth muscle alphaactin; however, the presence of desmin in the AP septum was not confirmed.…”

mentioning

confidence: 92%

Smooth muscle cells of neural crest origin form the aorticopulmonary septum in the avian embryo

Beall

Rosenquist

1990

Anat. Rec.

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Previous studies have shown that the cells of the aorticopulmonary (AP) septum are similar to the smooth muscle cells of the mediae of the great vessels in their common origin from the cardiac neural crest and in their common expression of an elastic extracellular matrix. The purpose of this study was to test the cells of the AP septum for the presence of certain cytoplasmic proteins, especially smooth muscle alpha-actin (SMAA) whose presence is definitive of smooth muscle. A monoclonal antibody against SMAA was applied to normal chicken embryos at 3.5-8 days of incubation and to age-matched embryos from which the cardiac neural crest had been ablated surgically. Antibodies against the intermediate filaments desmin, cytokeratin, and vimentin also were applied. The results showed that the AP septal cells expressed SMAA during the process of septation, days 5-8; but when the cardiac neural crest was ablated and septation was defective, no cells in the conotruncal connective tissue expressed SMAA. None of the intermediate filament proteins were detected in the septum. These results indicate that the AP septal cells are smooth muscle and therefore may be hypothesized to have an active role in septation.

show abstract

Desmin distribution in the cardiac outflow tract of the chick embryo during aortico-pulmonary septation.

Cited by 14 publications

References 16 publications

Human and rat hepatic stellate cells express neurotrophins and neurotrophin receptors

Human and rat hepatic stellate cells express neurotrophins and neurotrophin receptors

Synaptophysin: A Novel Marker for Human and Rat Hepatic Stellate Cells

Smooth muscle cells of neural crest origin form the aorticopulmonary septum in the avian embryo

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