Designing topical hyaluronic acid technology—Size does matter…

Widgerow, Alan D.; Ziegler, Mary E.; Garruto, John A; Robinson, Deanne Mraz; Palm, Melanie D.; Vega, Janelle H; Bell, Michaela

doi:10.1111/jocd.15027

Cited by 3 publications

(1 citation statement)

References 17 publications

(41 reference statements)

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“…To simulate the maternal environment in vitro, antioxidant drugs are added to the culture medium to reduce or eliminate the embryo blocks caused by environmental stressors. Melatonin (Choi et al., 2024; Lavrentiadou et al., 2023), vitamins (Georgiev et al., 2023; Widgerow et al., 2023), cysteines (Kim et al., 2023), and quercetin (Shi et al., 2023; Zhou et al., 2023) are examples of natural antioxidant substances that have been used in this context. Thus, the identification of novel natural antioxidant drugs is crucial for improving embryonic development and assisted reproductive technologies.…”

Section: Introductionmentioning

confidence: 99%

Mangiferin improves early porcine embryonic development by reducing oxidative stress

Ji,

Wang,

Yuan

et al. 2024

Reprod Domestic Animals

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Mangiferin (MGN) is primarily found in the fruits, leaves, and bark of plants of the Anacardiaceae family, including mangoes. MGN exhibits various pharmacological effects, such as protection of the liver and gallbladder, anti‐lipid peroxidation, and cancer prevention. This study aimed to investigate the effects of MGN supplementation during in vitro culture (IVC) on the antioxidant capacity of early porcine embryos and the underlying mechanisms involved. Porcine parthenotes in the IVC medium were exposed to different concentrations of MGN (0, 0.01, 0.1, and 1 μM). The addition of 0.1 μM MGN significantly increased the blastocyst formation rate of porcine embryos while reducing the apoptotic index and autophagy. Furthermore, the expression of antioxidation‐related (SOD2, GPX1, NRF2, UCHL1), cell pluripotency (SOX2, NANOG), and mitochondria‐related (TFAM, PGC1α) genes was upregulated. In contrast, the expression of apoptosis‐related (CAS3, BAX) and autophagy‐related (LC3B, ATG5) genes decreased after MGN supplementation. These findings suggest that MGN improves early porcine embryonic development by reducing oxidative stress‐related genes.

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Section: Introductionmentioning

confidence: 99%

Mangiferin improves early porcine embryonic development by reducing oxidative stress

Ji,

Wang,

Yuan

et al. 2024

Reprod Domestic Animals

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Designing topical hyaluronic acid technology—Size does matter…

Widgerow

Ziegler²,

Garruto³

et al. 2022

J of Cosmetic Dermatology

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Introduction: Hyaluronic acid (HA) plays an important role in cellular and extracellular matrix (ECM) homeostasis. Recent studies demonstrate that low molecular weight (MW) HA has pro-inflammatory characteristics while high MW HA is considered antiinflammatory and regenerative. In formulating a topical HA product, the possibility of creating a focused high MW HA technology was posed, combining external surface high MW HA constituents with active agents promoting fibroblast production of high MW in the depths of the dermis.Methods: Human dermal fibroblasts and keratinocytes were treated with various agents, and RNA sequencing (RNA-seq) was conducted to identify genes involved in HA synthesis. HA production by fibroblasts was assessed by collecting the culture supernatant, concentrating the protein, and conducting polyacrylamide gel electrophoresis (PAGE). The gel was stained with Stains-All to identify bands relative to known HA products of different MWs. Subsequently, the supernatants were treated with hyaluronidase to confirm the bands corresponded to HA. Results:The RNA-seq results revealed a variety of agents upregulated HA-related genes. However, a potent upregulation of HA synthesis gene was observed by hexapeptide-11 in the keratinocytes and a newly identified proprietary octapeptide in the fibroblasts. PAGE demonstrated not only robust production of HA by octapeptide, but significantly, the HA produced was ~2 Mega Daltons in size. Octapeptide was the most potent stimulator among the tested agents. Conclusion:Comprehensive in vitro testing identified a group of active agents that stimulated high MW HA production. This novel approach to HA topical application with exclusively high MW HA production should maximize hydration capacity while encouraging regenerative activity within the ECM. Multi-center trials are underway.

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Multicenter evaluation of a topical hyaluronic acid serum

Robinson

Vega

Palm

et al. 2022

J of Cosmetic Dermatology

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Background A new hyaluronic acid (HA) formulation was developed based on high molecular weight (MW) compounds used on the surface of the skin while using peptides to stimulate the high MW HA production by fibroblasts and keratinocytes from within the skin layers. Detailed science has been submitted to this journal in a previous publication. This multicenter study aims to validate the science by demonstrating the safety and efficacy of the product in the clinical realm. Objectives This study evaluated the efficacy and safety of a topical HA serum in facial skin. Methods An open‐label clinical study was undertaken over 4 months from November 2021 to March 2022. Participants applied the topical serum twice daily and were provided a gentle cleanser and an SPF 30+ to use in the morning. Follow‐up visits were conducted at weeks 2, 4, and 8. At every visit, participants were measured for hydration post 15 minutes of cleansing the skin and post 15 minutes of product application for cumulative skin hydration sensor measurements. Additional procedures included participant assessments and satisfaction, investigator assessments, biopsies, and photography. Results At each follow‐up visit, there was an increase in hydration measurements compared to baseline, in both immediate scores and cumulative long‐term scores. At weeks 4 and 8, there was a statistically significant increase in hydration compared to baseline and the prior visit. Participants' assessments progressively increased over 2‐, 4‐, and 8‐week intervals with significantly favorable ratings in all measured parameters. Similarly, investigator assessment grades were statistically significant (p < 0.0001) for decreased fine lines/wrinkling, crepiness, texture, erythema, and dryness, and increased (p < 0.0001) for moisture/hydration. Histology revealed increased CD44 staining in 6 of the 7 participants biopsied, denoting increased HA stimulation. In all of the participant biopsies, H&E staining demonstrated improvement in solar elastosis. Photography revealed remarkable improvement in erythema, tone, and texture. Conclusions The study results demonstrated that the formulation produced significant improvements in immediate and long‐term hydration effects on the skin as measured by the skin hydration sensor, ‘wearifi’ technology, comparison of before and after biopsies, and participant and investigator assessments. This high MW HA formulation produced excellent clinical improvement in skin health and hydration.

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Designing topical hyaluronic acid technology—Size does matter…

Cited by 3 publications

References 17 publications

Mangiferin improves early porcine embryonic development by reducing oxidative stress

Mangiferin improves early porcine embryonic development by reducing oxidative stress

Designing topical hyaluronic acid technology—Size does matter…

Multicenter evaluation of a topical hyaluronic acid serum

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