Designing Melittin‐Graphene Hybrid Complexes for Enhanced Antibacterial Activity

Lu, Xuemei; Liu, Jiaojiao; Gou, Lu; Li, Jingliang; Yuan, Bing; Yang, Kai; Ma, Yu‐qiang

doi:10.1002/adhm.201801521

Cited by 37 publications

(42 citation statements)

References 48 publications

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“…Additionally, this is consistent with the results that were focused on the antimicrobial properties of MEL. MEL hybrids with nGO and GN showed a 20-fold increase in antimicrobial activity [28]. MEL-GN and -nGO activity may result from colloid formation (zeta potecial: 23.9 and 36.8 mV), so the complexes were evenly dispersed in the medium with free access to the cells.…”

Section: Discussionmentioning

confidence: 99%

Use of Selected Carbon Nanoparticles as Melittin Carriers for MCF-7 and MDA-MB-231 Human Breast Cancer Cells

et al. 2019

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Despite advanced techniques in medicine, breast cancer caused the deaths of 627,000 women in 2018. Melittin, the main component of bee venom, has lytic properties for many types of cells, including cancer cells. To increase its toxic effect, carbon nanoparticles, graphene oxide, pristine graphene, and diamond were used as carriers of melittin to breast cancer cells. To date, the effects of carbon nanoparticles as carriers of melittin on cancer cells have not been studied. The present study was carried out on MCF-7 and MDA-MB-231 cell lines. The investigation consisted of structural analysis of complexes using transmission electron microscopy, zeta potential measurements, evaluation of cell morphology, assessment of cell viability and membrane integrity, investigation of reactive oxygen species production, and investigation of mitochondrial membrane potential. Cell death was examined by flow cytometry and a membrane test for 43 apoptotic proteins. The results indicate that melittin complex with nanographene oxide has a stronger toxic effect on breast cancer cells than melittin alone. Moreover, nanodiamonds can protect cells against the lytic effects of melittin. All complexes reduced, but not completely eliminated the level of necrosis, compared to melittin. Thus, results suggest that the use of carbon nanoparticles as carriers for melittin may find use in medicine in the future.

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Section: Discussionmentioning

confidence: 99%

Use of Selected Carbon Nanoparticles as Melittin Carriers for MCF-7 and MDA-MB-231 Human Breast Cancer Cells

et al. 2019

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“…The improved bacteria-killing ability is mostly induced by the increased local peptide concentration of AMPs, which strengthens the potential cooperation between peptides. Very recently, the in silico design of a hybrid complex composed of melittin and graphene materials (e.g., graphene or graphene oxide nanosheets) was reported [116]. Simulations and experiments showed that the as-fabricated complex exhibited remarkable efficiency in transmembrane perforation due to the synergetic insertion of graphene materials and melittin into membranes.…”

Section: Discussionmentioning

confidence: 99%

How Melittin Inserts into Cell Membrane: Conformational Changes, Inter-Peptide Cooperation, and Disturbance on the Membrane

Hong

Deng

et al. 2019

Molecules

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Antimicrobial peptides (AMPs), as a key component of the immune defense systems of organisms, are a promising solution to the serious threat of drug-resistant bacteria to public health. As one of the most representative and extensively studied AMPs, melittin has exceptional broad-spectrum activities against microorganisms, including both Gram-positive and Gram-negative bacteria. Unfortunately, the action mechanism of melittin with bacterial membranes, especially the underlying physics of peptide-induced membrane poration behaviors, is still poorly understood, which hampers efforts to develop melittin-based drugs or agents for clinical applications. In this mini-review, we focus on recent advances with respect to the membrane insertion behavior of melittin mostly from a computational aspect. Membrane insertion is a prerequisite and key step for forming transmembrane pores and bacterial killing by melittin, whose occurrence is based on overcoming a high free-energy barrier during the transition of melittin molecules from a membrane surface-binding state to a transmembrane-inserting state. Here, intriguing simulation results on such transition are highlighted from both kinetic and thermodynamic aspects. The conformational changes and inter-peptide cooperation of melittin molecules, as well as melittin-induced disturbances to membrane structure, such as deformation and lipid extraction, are regarded as key factors influencing the insertion of peptides into membranes. The associated intermediate states in peptide conformations, lipid arrangements, membrane structure, and mechanical properties during this process are specifically discussed. Finally, potential strategies for enhancing the poration ability and improving the antimicrobial performance of AMPs are included as well.

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“…Graphene materials, especially graphene or GO nanosheets, can be inserted into the membrane through their sharp edges and corners, resulting in increased membrane stress or intracellular material leakage to achieve the antibacterial effects ( Li et al, 2013b ), which are ideal materials to integrate with AMPs for an enhanced antibacterial activity. Lu and colleagues designed a hybrid compound of AMP melittin and graphene/GO nanosheets to achieve a preferable antibacterial effect, which showed significant efficiency in transmembrane perforation compared with the original melittin and hoisted the antibacterial capacity against Gram-positive and Gram-negative bacteria by 20 times ( Lu et al, 2019 ). Ren et al synthesized an AMP-grafted GO nanosheets complex, i.e., D28@GO, which possessed a strong effect on inhibiting the growth of pathogens such as C. Albicans and E. coli etc., and suppressed systemic infection of C. Albicans in vivo remarkably ( Ren et al, 2019 ).…”

Section: Graphene Biosensors For Pathogensmentioning

confidence: 99%

Graphene biosensors for bacterial and viral pathogens

Jiang

Feng

et al. 2020

Biosensors and Bioelectronics

124

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: The infection and spread of pathogens (e.g., COVID-19) pose an enormous threat to the safety of human beings and animals all over the world. The rapid and accurate monitoring and determination of pathogens are of great significance to clinical diagnosis, food safety and environmental evaluation. In recent years, with the evolution of nanotechnology, nano-sized graphene and graphene derivatives have been frequently introduced into the construction of biosensors due to their unique physicochemical properties and biocompatibility. The combination of biomolecules with specific recognition capabilities and graphene materials provides a promising strategy to construct more stable and sensitive biosensors for the detection of pathogens. This review tracks the development of graphene biosensors for the detection of bacterial and viral pathogens, mainly including the preparation of graphene biosensors and their working mechanism. The challenges involved in this field have been discussed, and the perspective for further development has been put forward, aiming to promote the development of pathogens sensing and the contribution to epidemic prevention.

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Designing Melittin‐Graphene Hybrid Complexes for Enhanced Antibacterial Activity

Cited by 37 publications

References 48 publications

Use of Selected Carbon Nanoparticles as Melittin Carriers for MCF-7 and MDA-MB-231 Human Breast Cancer Cells

Use of Selected Carbon Nanoparticles as Melittin Carriers for MCF-7 and MDA-MB-231 Human Breast Cancer Cells

How Melittin Inserts into Cell Membrane: Conformational Changes, Inter-Peptide Cooperation, and Disturbance on the Membrane

Graphene biosensors for bacterial and viral pathogens

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