Designing materials to direct stem-cell fate

Lütolf, Matthias P.; Gilbert, Penney M.; Blau, Helen M.

doi:10.1038/nature08602

Cited by 1,259 publications

(1,051 citation statements)

References 86 publications

Supporting

Mentioning

1,037

Contrasting

Unclassified

Order By: Relevance

“…Substrate stiffness and focal polarization of progenitor cells in conventional 2-D culture conditions dramatically affect the fate and physiology of cells [22,38]. The 3-D platform that we generated allows tuning of both hydrogel stiffness and cell encapsulation density.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies investigating cranial neural crest cell biology utilize 2-D monolayer culture models, which do not replicate the native, three-dimensionally defined microenvironments [21][22][23]. In this study, we created a broadly applicable platform that allows the direct differentiation of multipotent progenitor cells, including those of neural crest origin.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Recapitulating cranial osteogenesis with neural crest cells in 3-D microenvironments

et al. 2016

View full text Add to dashboard Cite

The experimental systems that recapitulate the complexity of native tissues and enable precise control over the microenvironment are becoming essential for the pre-clinical tests of therapeutics and tissue engineering. Here, we described a strategy to develop an in vitro platform to study the developmental biology of craniofacial osteogenesis. In this study, we directly osteo-differentiated cranial neural crest cells (

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Recapitulating cranial osteogenesis with neural crest cells in 3-D microenvironments

et al. 2016

View full text Add to dashboard Cite

show abstract

“…While the importance of ECM protein interactions is well recognized, this has been emphasized in recent times by the role they play in regulating stem cell niches 5 . In these niches, bound ECM protein and other growth factors, not just soluble molecules, are increasingly implicated as critical regulators of spatial and temporal determination of stem cell fate.…”

Section: Introductionmentioning

confidence: 99%

Quantifying fibronectin adhesion with nanoscale spatial resolution on glycosaminoglycan doped polypyrrole using Atomic Force Microscopy

Gelmi

Higgins

Wallace

2013

Biochimica et Biophysica Acta (BBA) - General Subjects

View full text Add to dashboard Cite

The interaction of ECM proteins is critical in determining the performance of materials used in biomedical applications such as tissue regeneration, implantable bionics and biosensing. Methods: To improve our understanding of ECM protein-conducting polymer interactions, we have used Atomic Force Microscopy (AFM) to elucidate the interactions of fibronectin (FN) on polypyrrole (PPy) doped with different glycosaminoglycans. Results: We were able to classify four main types of FN interactions, including those related to 1) non-specific adhesion, 2) protein unfolding and subsequent unbinding from the surface, 3) desorption and 4) interactions with no adhesion. FN adhesion on PPy/hyaluronic acid showed a significantly lower density of surface adhesion with the adhesion restricted to nodule structures, as opposed to their peripheries, of the polymer morphology. In contrast, PPy/chondroitin sulfate showed a significantly higher density of surface adhesion to the point where the distribution of adhesion effectively masked the topography. Through conductive AFM imaging, we found that the conductive regions correlated with regions of FN adhesion. Conclusions: Given that the conductivity requires doping of the polymer, these findings suggest that FN adhesion is mediated by interactions with chondroitin sulfate and hyaluronic acid at the polymer surface and may be indicative of specific interactions due to contributions from electrostatic attraction between the FN and sulfate/anionic groups of the dopants. General significance: This study demonstrates the ability of AFM to resolve the protein-conducting polymer interactions at the molecular and nanoscale level, which will be important for interfacing these polymer materials with biological systems. This article is part of a Special Issue entitled Organic Bioelectronics -Novel Applications in Biomedicine.

show abstract

“…To address this arising need, we developed a simple, fast, and scalable method that uses standard microarray printing tools to generate high-density nanowell arrays. A minimal sample requirement of 500 cells enables the interrogation of cells that are available in limited quantities or cells that cannot be expanded due to undesirable changes in phenotype or genotype, as in the case of stem cells and cancer cell lines 12 .…”

mentioning

confidence: 99%

Live mammalian cell arrays

et al. 2013

View full text Add to dashboard Cite

High-content assays have the potential to drastically increase throughput in cell biology and drug discovery, but handling and culturing large libraries of cells such as primary tumor or cancer cell lines requires expensive, dedicated robotic equipment. We have developed a simple, yet powerful method that uses contact spotting to generate highdensity nanowell arrays of live mammalian cells for the culture and interrogation of cell libraries. High-content assays have the potential to drastically increase throughput in cell biology and drug discovery, but handling and culturing large libraries of cells such as primary tumor or cancer cell lines requires expensive, dedicated robotic equipment. We have developed a simple, yet powerful method that uses contact spotting to generate highdensity nanowell arrays of live mammalian cells for the culture and interrogation of cell libraries.Cell-based assays and the tools used to perform them are constantly undergoing improvements toward higher experimental throughput, reduced reagent consumption, and advanced control of the cell microenvironment 1,2 . There are currently two main approaches for conducting highcontent cell culture experiments. In the first, cells are cultured in microtiter plates and robotic equipment is used to perform all necessary fluidic operations 3 . Although effective, this method remains unavailable to many laboratories due to the requirement for expensive robotics. A second approach that can be used for high-throughput cell studies is reverse transfection [4][5][6] . Here, cells are seeded onto an array of DNA, RNA, or small molecules. Large gene expression and silencing studies can be conducted in this manner, but complex cell libraries cannot be investigated since each array is limited to a single cell type.As an alternative to these approaches, contact spotting offers an affordable yet high-throughput platform. This technique is extensively used to generate high-density DNA and protein arrays and has been adapted to a variety of other purposes. For example, Hart et al. created a high-content immunoassay by spotting fixed mammalian cells that were cultured under different conditions 7 . To date, contact spotting has not been used to array live mammalian cells due to rapid spot evaporation and consequent cell death. Therefore, arraying of live mammalian cells has been limited to inkjet printing, which lacks the ability to handle a large number of different samples 8,9 .Large collections of mammalian cell lines have recently become available, including primary tumor and cancer cell lines 10 , stably transfected expression cell lines, and GFP-fusion libraries 11 . Novel approaches are required to efficiently assemble complex arrays of hundreds to thousands of genetically diverse cells. To address this arising need, we developed a simple, fast, and scalable method that uses standard microarray printing tools to generate high-density nanowell arrays. A minimal sample requirement of 500 cells enables the interrogation of cells that are availab...

show abstract

Designing materials to direct stem-cell fate

Cited by 1,259 publications

References 86 publications

Recapitulating cranial osteogenesis with neural crest cells in 3-D microenvironments

Recapitulating cranial osteogenesis with neural crest cells in 3-D microenvironments

Quantifying fibronectin adhesion with nanoscale spatial resolution on glycosaminoglycan doped polypyrrole using Atomic Force Microscopy

Live mammalian cell arrays

Contact Info

Product

Resources

About