Designing a Rapid, Reliable and Reproducible Method for the Detection of Salmonella Spp. From Poultry Meat

Fatima, Aiman; Nawaz, Shahid; Shahid, Maryam; Saleem, Maira; Fatima, Iza

doi:10.52700/jmmg.v3i2.51

Cited by 3 publications

(1 citation statement)

References 13 publications

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“…Bacteria can colonize a wide range of animals, serving as reservoirs and vectors for the transmission of these infections to both animal and human populations. S. enterica serovars are still among the world’s most prevalent food-borne pathogens ( Fatima et al, 2022 ). Public concern over antibiotic-resistant strains, especially among zoonotic pathogens like Salmonella , has driven the poultry sector to identify alternative control methods ( Boyle et al, 2007 ).…”

Section: Phage Therapy Of Bacterial Infections and Zoonotic Agents In...mentioning

confidence: 99%

An overview of the use of bacteriophages in the poultry industry: Successes, challenges, and possibilities for overcoming breakdowns

et al. 2023

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The primary contaminants in poultry are Salmonella enterica, Campylobacter jejuni, Escherichia coli, and Staphylococcus aureus. Their pathogenicity together with the widespread of these bacteria, contributes to many economic losses and poses a threat to public health. With the increasing prevalence of bacterial pathogens being resistant to most conventional antibiotics, scientists have rekindled interest in using bacteriophages as antimicrobial agents. Bacteriophage treatments have also been investigated as an alternative to antibiotics in the poultry industry. Bacteriophages’ high specificity may allow them only to target a specific bacterial pathogen in the infected animal. However, a tailor-made sophisticated cocktail of different bacteriophages could broaden their antibacterial activity in typical situations with multiple clinical strains infections. Bacteriophages may not only be used in terms of reducing bacterial contamination in animals but also, under industrial conditions, they can be used as safe disinfectants to reduce contamination on food-contact surfaces or poultry carcasses. Nevertheless, bacteriophage therapies have not been developed sufficiently for widespread use. Problems with resistance, safety, specificity, and long-term stability must be addressed in particular. This review highlights the benefits, challenges, and current limitations of bacteriophage applications in the poultry industry.

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Section: Phage Therapy Of Bacterial Infections and Zoonotic Agents In...mentioning

confidence: 99%

An overview of the use of bacteriophages in the poultry industry: Successes, challenges, and possibilities for overcoming breakdowns

et al. 2023

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Prevalence and antibiotics resistance status of Salmonella in raw meat consumed in various areas of Lahore, Pakistan

Fatima,

Saleem,

Nawaz

et al. 2023

Sci Rep

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This study reports the prevalence and antibiotics resistance status of Salmonella detected in raw meat from Lahore, Pakistan. Overall, N = 111 meat samples, were collected from local markets. Salmonella was recovered from 57 (51.35%) samples, including 45.83% of poultry, 60% of buffalo, 64.28% of cow, and 60% of goat meat samples. The predominant Salmonella strains were Salmonella enterica serovars; Typhimurium (45.4%), Typhi (27.2%), and Enteritidis (18.1%), identified by VITEK system and 16S rRNA gene sequencing. The isolates exhibited high resistance to Erythromycin (100%), Cefepime (98.24%), Colistin (94.73%), Azithromycin (92.98%), Tetracycline (87.71%), Polymyxin B (84.21%), Ciprofloxacin (84.21%), Trimethoprim-Sulfamethoxazole (80.70%), Nalidixic Acid (80.70%), Kanamycin (78.94%), Chloramphenicol (77.19%), Streptomycin (71.92%) and Ampicillin (64.91%). While the isolates exhibited more susceptibility to Meropenem (75.43%) and Amikacin (73.68%). N = 8 strains were designated as Multidrug Resistant (MDR) and N = 3 as Extensively Drug-Resistant (XDR) Salmonella. The PCR-based detection of resistance genes revealed the presence of blaTEM-1 gene (100%), catA1 gene (64%), and gyrA gene (18%). The whole genome sequencing (WGS) of two selected strains and subsequent downstream analysis confirmed the strains as MDR and XDR Salmonella enterica serovar Typhi. The study showed that raw meat consumed in Lahore carries a significantly high number of drug-resistant Salmonella.

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Virulence and Antimicrobial‐Resistant Gene Profiles of Salmonella spp. Isolates from Chicken Carcasses Markets in Ibague City, Colombia

Lozano-Villegas,

Rondón-Barragán

2024

International Journal of Microbiology

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Salmonella spp. is one of the leading causes of foodborne bacterial infections, with major impacts on public health and healthcare system. Salmonella is commonly transmitted via the fecal‐to‐oral route, and food contaminated with the bacteria (e.g., poultry products) is considered a common source of infection, being a potential risk for public health. The study aims to characterize the antimicrobial resistance‐ and virulence‐associated genes in Salmonella isolates recovered from chicken marketed carcasses (n = 20). The presence of 14 antimicrobial and 23 virulence genes was evaluated using end‐point PCR. The antimicrobial genes were detected in the following proportion among the isolates: blaTEM 100%, dfrA1 and blaCMY2 90% (n = 18), aadA1 75% (n = 15), sul1 and sul2 50% (n = 10), floR 45% (n = 9), qnrD 20% (n = 4), and aadA2 15% (n = 3). catA, sul3, qnrS, and aac(6′)-Ib genes were absent in all isolates. Regarding virulence‐associated genes, all Salmonella strains contain invA, fimA, avrA, msgA, sopB, and sopE. The cdtB gene was present in 95% (n = 19) of isolates, whereas spvC and spvB were present in 55% (n = 11). Other virulence genes such as spiC, lpfC, lpfA, and csgA were present in 90% (n = 18) of strains. The presence of antimicrobial and virulence genes in several Salmonella strains in chicken meat suggests the potential pathogenicity of the strains, which is relevant given the possibility of cross‐contamination which represents a significant threat to public health.

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Designing a Rapid, Reliable and Reproducible Method for the Detection of Salmonella Spp. From Poultry Meat

Cited by 3 publications

References 13 publications

An overview of the use of bacteriophages in the poultry industry: Successes, challenges, and possibilities for overcoming breakdowns

An overview of the use of bacteriophages in the poultry industry: Successes, challenges, and possibilities for overcoming breakdowns

Prevalence and antibiotics resistance status of Salmonella in raw meat consumed in various areas of Lahore, Pakistan

Virulence and Antimicrobial‐Resistant Gene Profiles of Salmonella spp. Isolates from Chicken Carcasses Markets in Ibague City, Colombia

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