Designing a Polymerase Chain Reaction Device Working with Radiation and Convection Heat Transfer

Madadelahi, Masoud; Kalan, Kian; Shamloo, Amir

doi:10.1088/1757-899x/350/1/012010

Cited by 2 publications

(1 citation statement)

References 11 publications

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“…In recent decades, considerable efforts have been made regarding particle production and handling in microfluidic systems for a broad range of applications ranging from water treatment [1] to cell study and clinical diagnostics [2,3]. The advantages of using microfluidic systems include the low sample and reagent consumption, efficient heat and mass transfer, coexistence of different fluid phases, improved detection limits, compact size, shorter processing time, high-throughput capability, portability of the instrumentation, enhanced operational flexibility, and reduced costs by integrating a full set of operations on a single chip [4][5][6][7][8]. Droplet microfluidics offers exquisite simultaneous control over multiple fluids by allowing precise tuning of both the geometric characteristics and the compositions of the droplets used to synthesize microparticles.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Fluidic-Barrier-Based Particle Generation in Centrifugal Microfluidics

et al. 2022

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The fluidic barrier in centrifugal microfluidic platforms is a newly introduced concept for making multiple emulsions and microparticles. In this study, we focused on particle generation application to better characterize this method. Because the phenomenon is too fast to be captured experimentally, we employ theoretical models to show how liquid polymeric droplets pass a fluidic barrier before crosslinking. We explain how secondary flows evolve and mix the fluids within the droplets. From an experimental point of view, the effect of different parameters, such as the barrier length, source channel width, and rotational speed, on the particles' size and aspect ratio are investigated. It is demonstrated that the barrier length does not affect the particle's ultimate velocity. Unlike conventional air gaps, the barrier length does not significantly affect the aspect ratio of the produced microparticles. Eventually, we broaden this concept to two source fluids and study the importance of source channel geometry, barrier length, and rotational speed in generating two-fluid droplets.

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