Designing a direct ELISA kit for the detection of Staphylococcus aureus enterotoxin A in raw milk samples

Nouri, Arezoo; Ahari, Hamed; Shahbazzadeh, Delavar

doi:10.1016/j.ijbiomac.2017.10.052

Cited by 42 publications

(18 citation statements)

References 16 publications

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“…Immunoassay has become the main tool for rapid detection of harmful toxigenic bacteria due to its high specificity for toxicity. Nouri et al (2018) designed a new kit for the detection of SEA in milk, with a detection time of about 15 min and a sensitivity of 15.6 ng of toxin. For instance, Sun et al (2020) developed a sandwich chemiluminescent immunoassay (CLIA) to detect SEB, an anti-SEB monoclonal antibody was used as the capture antibody, and Nb37-ALP was used as the detector antibody; the detection limit was 1.44 ng/ml.…”

Section: Available Methods For Detecting S Aureusmentioning

confidence: 99%

Aptasensors for Staphylococcus aureus Risk Assessment in Food

Huang

Yang

et al. 2021

Front. Microbiol.

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Staphylococcus aureus (S. aureus) is the top ordinary pathogen causing epidemic and food poisoning. The authentication of S. aureus has great significance for pathologic diagnosis and food hygiene supervision. Various biosensor methods have been established for identification. This paper reviews the research progress of aptasensors for S. aureus detection, focusing on the classification of aptamer technologies, including optical aptasensors and electrochemical aptasensors. Furthermore, the feasibility and future challenges of S. aureus detection for aptamer assays are discussed. Combining aptasensors with nanomaterials appears to be the developing trend in aptasensors.

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Section: Available Methods For Detecting S Aureusmentioning

confidence: 99%

Aptasensors for Staphylococcus aureus Risk Assessment in Food

Huang

Yang

et al. 2021

Front. Microbiol.

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“…Stef et al [42] developed a sensitive and specific ELISA test for measuring the amount of sesame seed residue, which could provide a screening tool for foodstuffs for highly allergic patients. ELISA tests can also detect foodborne pathogens, such as Salmonella [43], Escherichia coli O157:H7 [44], Vibrio parahaemolyticus [45], Listeria [46], and Staphylococcus aureus [47]. In addition, ELISAs are also used for the detection of toxins present in foods, such as Clostridium perfringens α and β toxins, and E. coli enterotoxins [41,48].…”

Section: Other Applications In Future Food Productionmentioning

confidence: 99%

Current progress and prospects of enzyme technologies in future foods

Pang

Yin

Zhang

et al. 2020

Syst Microbiol and Biomanuf

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Enzyme technologies are widely used in the food industry due to their advantages of high efficiency, specificity, and safety. Recently, "future foods" is emerging as a new research hotspot with healthier foods that are more nutritious, delicious, and sustainable; however, these foods still have problems with texture, nutrition, and flavor. Advances in enzyme technology have enabled the development of new tools and approaches to better manipulate food textures and nutritional aspects. In this review, we summarize enzyme technology applications in future food production, focusing on food texture, safety, and flavors. Furthermore, we discuss the prospects of enzyme-based technologies for future food production, including the modification of enzyme activities, the development of suitable food-grade hosts for enzyme production, and the optimization synergistic multi-enzyme systems.

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“…However, these assays are relatively complicated and time-consuming. Therefore, more rapid and simple methods are warranted, mainly includes immunology and molecular biology (Amani et al, 2015;Chen et al, 2015;Maerle et al, 2014;Nouri et al, 2018;Shao et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Development of multiplex loop-mediated isothermal amplification for three foodborne pathogens

Xu¹,

Luo

Zhang

2020

Food Sci. Technol

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Staphylococcus aureus, Salmonella, and Shigella are three major foodborne pathogenic microorganisms that cause global public health problems. We developed a multiplex loop-mediated isothermal amplification (mLAMP) assay for simultaneous detection of S. aureus nuc, Salmonella fimY, and Shigella ipaH in fresh fruit juice using three sets of primers. On addition, three different restriction enzyme cleavage sites were designed in each forward inner primer (FOP), namely, XhoO in nuc FOP, KpnO in fimY FOP, and BamHO in ipaH FOP. DNA was amplified using the LAMP assay at 64 °C for 50 min followed by endonuclease restriction digestion to separate the LAMP products of three pathogens. The minimum amount of genomic DNA of S. aureus, Salmonella, and Shigella that could be detected by mLAMP was 100 fg/25 μL, whereas for mPCR, it was 1 pg/25 μL. The artificially contaminated juice can also be detected by mLAMP after enrichment, which had the limit of detection (LDD) of 2 CFU/10 mL. On conclusion, the mLAMP developed in this study could be potentially used in the detection of S. aureus, Salmonella, and Shigella in food, particularly as a primary screening method in developing areas.

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Designing a direct ELISA kit for the detection of Staphylococcus aureus enterotoxin A in raw milk samples

Cited by 42 publications

References 16 publications

Aptasensors for Staphylococcus aureus Risk Assessment in Food

Aptasensors for Staphylococcus aureus Risk Assessment in Food

Current progress and prospects of enzyme technologies in future foods

Development of multiplex loop-mediated isothermal amplification for three foodborne pathogens

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