Abstract:Staphylococcus aureus, Salmonella, and Shigella are three major foodborne pathogenic microorganisms that cause global public health problems. We developed a multiplex loop-mediated isothermal amplification (mLAMP) assay for simultaneous detection of S. aureus nuc, Salmonella fimY, and Shigella ipaH in fresh fruit juice using three sets of primers. On addition, three different restriction enzyme cleavage sites were designed in each forward inner primer (FOP), namely, XhoO in nuc FOP, KpnO in fimY FOP, and BamHO… Show more
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