Abstract:Synthetic biology has enabled the production of many value-added chemicals via microbial fermentation. However,the problem of lowproduct titers from recombinant pathwaysh as limited the utility of this approach.M ethods to increase metabolic flux are therefore critical to the success of metabolic engineering.H ere we demonstrate that vitamin Ederived designer micelles,o riginally developed for use in synthetic chemistry,a re biocompatible and accelerate flux through astyrene production pathway in Escherichia c… Show more
“…Accumulation of high concentration of extracellular Monascus pigments was realized due to the plant oil phase with high extractive capacity. Furthermore, instable/toxic extracellular product can also be converted into relatively stable/nontoxic compound by enzymatic reaction (Willrodt et al 2015 ) or non-enzymatic reaction (Xiong et al 2015 ; Domaille et al 2016 ; Wallace and Balskus 2016 ), which may be a more efficient strategy to make whole cell biocatalyst play its potential.…”
Section: Discussionmentioning
confidence: 99%
“…In-situ product removal, such as addition of solid-state adsorbent (Evanst and Wang 1984 ), nonaqueous two-phase extraction (Wang and Dai 2010 ), is the most common strategy for elimination of product inhibition/degradation. Cascade conversion of instable/toxic product into stable/non-toxic compound by enzymatic reaction (Willrodt et al 2015 ) or non-enzymatic reaction (Xiong et al 2015 ; Domaille et al 2016 ; Wallace and Balskus 2016 ) is also developed for bioprocess optimization.…”
Cell suspension culture using mycelia as whole cell biocatalyst for production of orange Monascus pigments has been carried out successfully in a nonionic surfactant micelle aqueous solution. Thus, selection of mycelia as whole cell biocatalyst and the corresponding enzymatic kinetics for production of orange Monascus pigments can be optimized independently. Mycelia selected from submerged culture in a nonionic surfactant micelle aqueous solution with low pH 2.5 exhibits robust bioactivity. At the same time, enzymatic kinetic study shows that the bioactivity of mycelia as whole cell biocatalyst is sensitive to high product concentration. Segregation of product from mycelia by cell suspension culture in a nonionic surfactant micelle aqueous solution or peanut oil–water two-phase system is not only necessary for studying the enzymatic kinetics but also beneficial to industrial application of mycelia as whole cell biocatalyst.
“…Accumulation of high concentration of extracellular Monascus pigments was realized due to the plant oil phase with high extractive capacity. Furthermore, instable/toxic extracellular product can also be converted into relatively stable/nontoxic compound by enzymatic reaction (Willrodt et al 2015 ) or non-enzymatic reaction (Xiong et al 2015 ; Domaille et al 2016 ; Wallace and Balskus 2016 ), which may be a more efficient strategy to make whole cell biocatalyst play its potential.…”
Section: Discussionmentioning
confidence: 99%
“…In-situ product removal, such as addition of solid-state adsorbent (Evanst and Wang 1984 ), nonaqueous two-phase extraction (Wang and Dai 2010 ), is the most common strategy for elimination of product inhibition/degradation. Cascade conversion of instable/toxic product into stable/non-toxic compound by enzymatic reaction (Willrodt et al 2015 ) or non-enzymatic reaction (Xiong et al 2015 ; Domaille et al 2016 ; Wallace and Balskus 2016 ) is also developed for bioprocess optimization.…”
Cell suspension culture using mycelia as whole cell biocatalyst for production of orange Monascus pigments has been carried out successfully in a nonionic surfactant micelle aqueous solution. Thus, selection of mycelia as whole cell biocatalyst and the corresponding enzymatic kinetics for production of orange Monascus pigments can be optimized independently. Mycelia selected from submerged culture in a nonionic surfactant micelle aqueous solution with low pH 2.5 exhibits robust bioactivity. At the same time, enzymatic kinetic study shows that the bioactivity of mycelia as whole cell biocatalyst is sensitive to high product concentration. Segregation of product from mycelia by cell suspension culture in a nonionic surfactant micelle aqueous solution or peanut oil–water two-phase system is not only necessary for studying the enzymatic kinetics but also beneficial to industrial application of mycelia as whole cell biocatalyst.
“…Additionally, modification of metabolites can be an effective method of driving flux through a metabolic pathway towards the product of interest. 51 Furthermore, microbes are able to alter the chemistry of their external environment ( e.g. pH and/or O 2 levels 52 ), potentially facilitating non-enzymatic reactions.…”
Section: Biocompatible Chemistry For Small Molecule Synthesismentioning
This review highlights recent advances in the field of biocompatible chemistry. It focusses on the combined use of non-enzymatic catalysis and microbial metabolism to support cellular function and to synthesise high value chemicals.
“…The extracellular location of transition metal catalysts has been successfully combined with biosynthetic pathways to catalyse the final step in several biosynthetic cascades (Figure 4). This approach has been used to make feedstock chemicals (Figure 4B) [52], small-molecule intermediates (Figure 4C) [53] as well as potential antibiotics (Figure 4D) [54]. Interestingly, as far as the author is aware no engineered metalloenzyme or ArM catalysing an abiotic reaction have been included in a biosynthetic pathway, even though their use in vivo has been described for both ring-closing metathesis and cyclopropanation, as featured in Figure 4B and C.…”
Section: Cascade Reactions For Chemical Synthesismentioning
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