Designer DNA–silica/carbon nanotube nanocomposites for traceable and targeted drug delivery

Hu, Yong; Niemeyer, Christof M.

doi:10.1039/c9tb02861g

Cited by 41 publications

(26 citation statements)

References 22 publications

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“…Z-stack image analyses indicated that the composite materials are located inside the cells ( Supplementary Materials Figure S2a,c ). Importantly, since the composites are not cytotoxic [ 29 , 32 ], the cells were able to continue dividing, while the ingested material was evenly distributed to the daughter cells ( Figure 3 c,d; see also Supplementary Materials Video S1 ).…”

Section: Resultsmentioning

confidence: 99%

“…These SiNP/CNT–DNA composites were recently developed in our group as programmable and cell-instructive biocoatings whose components and mechanical properties can be tailored by varying the concentrations of SiNP and CNT to influence the behavior of living cells [ 29 , 30 , 31 ]. In addition, these composites are readily taken up by eukaryotic cells and can thus be used for traceable and targeted drug delivery [ 32 ]. We report here for the first time the formulation of SiNP/CNT–DNA composites as bulk- and micro-formulated gels containing transcribable genetic information ( Figure 1 ).…”

Section: Introductionmentioning

confidence: 99%

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Formulation of DNA Nanocomposites: Towards Functional Materials for Protein Expression

Schipperges

Moench

et al. 2021

Polymers

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DNA hydrogels are an emerging class of materials that hold great promise for numerous biotechnological applications, ranging from tissue engineering to targeted drug delivery and cell-free protein synthesis (CFPS). In addition to the molecular programmability of DNA that can be used to instruct biological systems, the formulation of DNA materials, e.g., as bulk hydrogels or microgels, is also relevant for specific applications. To advance the state of knowledge in this research area, the present work explores the scope of a recently developed class of complex DNA nanocomposites, synthesized by RCA polymerization of DNA-functionalized silica nanoparticles (SiNPs) and carbon nanotubes (CNTs). SiNP/CNT–DNA composites were produced as bulk materials and microgels which contained a plasmid with transcribable genetic information for a fluorescent marker protein. Using confocal microscopy and flow cytometry, we found that the materials are very efficiently taken up by various eukaryotic cell lines, which were able to continue dividing while the ingested material was evenly distributed to the daughter cells. However, no expression of the encoded protein occurred within the cells. While the microgels did not induce production of the marker protein even in a CFPS procedure with eukaryotic cell lysate, the bulk composites proved to be efficient templates for CFPS. This work contributes to the understanding of the molecular interactions between DNA composites and the functional cellular machinery. Implications for the use of such materials for CFPS procedures are discussed.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Formulation of DNA Nanocomposites: Towards Functional Materials for Protein Expression

Schipperges

Moench

et al. 2021

Polymers

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“…Herein, we recommend that the order of treatment should be “first cancer, second repair.” Healing is a long process, while killing process is best shortened to prevent newborn normal cells from ablation together with cancer cells and to minimize the damage to the body. Besides, by encapsulating them into micro‐ or nano‐particles, 205 the delayed release of tissue repair biomolecules or stimulating factors can be realized. Moreover, multifunctional drugs with tumor‐killing capacity and histogenesis stimulating ability might be preferred.…”

Section: Discussionmentioning

confidence: 99%

Advances and trends of hydrogel therapy platform in localized tumor treatment: A review

Tan

Huang

et al. 2020

J Biomedical Materials Res

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Due to limitations of treatment and the stubbornness of infiltrative tumor cells, the outcome of conventional antitumor treatment is often compromised by a variety of factors, including severe side effects, unexpected recurrence, and massive tissue loss during the treatment. Hydrogel‐based therapy is becoming a promising option of cancer treatment, because of its controllability, biocompatibility, high drug loading, prolonged drug release, and specific stimuli‐sensitivity. Hydrogel‐based therapy has good malleability and can reach some areas that cannot be easily touched by surgeons. Furthermore, hydrogel can be used not only as a carrier for tumor treatment agents, but also as a scaffold for tissue repair. In this review, we presented the latest researches in hydrogel applications of localized tumor therapy and highlighted the recent progress of hydrogel‐based therapy in preventing postoperative tumor recurrence and improving tissue repair, thus proposing a new trend of hydrogel‐based technology in localized tumor therapy. And this review aims to provide a novel reference and inspire thoughts for a more accurate and individualized cancer treatment.

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“…[21]. Hu and Niemeyer proposed a novel hydrogel material composed of silica nanoparticles, CNT and DNA fragments rich in CG/GC sequences and showed its ability to deliver DOX molecules to HeLa cells [28]. Therefore, we can conclude from that short literature review that mainly silica or gold nanoparticles are considered as 'cores' of drug carriers utilizing the folding/unfolding property of iM sequences.…”

Section: Introductionmentioning

confidence: 99%

Cytosine-Rich DNA Fragments Covalently Bound to Carbon Nanotube as Factors Triggering Doxorubicin Release at Acidic pH. A Molecular Dynamics Study

Wolski

Nieszporek

Pańczyk

2021

IJMS

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This works deals with analysis of properties of a carbon nanotube, the tips of which were functionalized by short cytosine-rich fragments of ssDNA. That object is aimed to work as a platform for storage and controlled release of doxorubicin in response to pH changes. We found that at neutral pH, doxorubicin molecules can be intercalated between the ssDNA fragments, and formation of such knots can effectively block other doxorubicin molecules, encapsulated in the nanotube interior, against release to the bulk. Because at the neutral pH, the ssDNA fragments are in form of random coils, the intercalation of doxorubicin is strong. At acidic pH, the ssDNA fragments undergo folding into i-motifs, and this leads to significant reduction of the interaction strength between doxorubicin and other components of the system. Thus, the drug molecules can be released to the bulk at acidic pH. The above conclusions concerning the storage/release mechanism of doxorubicin were drawn from the observation of molecular dynamics trajectories of the systems as well as from analysis of various components of pair interaction energies.

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Designer DNA–silica/carbon nanotube nanocomposites for traceable and targeted drug delivery

Abstract: A programmable drug delivery system can be synthesized in a highly modular fashion from DNA-functionalized carbon nanotubes and silica nanoparticles via enzymatic rolling circle amplification.

Cited by 41 publications

References 22 publications

Formulation of DNA Nanocomposites: Towards Functional Materials for Protein Expression

Formulation of DNA Nanocomposites: Towards Functional Materials for Protein Expression

Advances and trends of hydrogel therapy platform in localized tumor treatment: A review

Cytosine-Rich DNA Fragments Covalently Bound to Carbon Nanotube as Factors Triggering Doxorubicin Release at Acidic pH. A Molecular Dynamics Study

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