Design, synthesis and immunological evaluation of self-assembled antigenic peptides from dual-antigen targets: a broad-spectrum candidate for an effective antibreast cancer therapy

Abstract

BackgroundConsidering the narrow immune response spectrum of a single epitope, and the nanoparticles (NPs) as a novel adjuvant can achieve efficient delivery of antigenic peptides safely, a nano-system (denoted as DSPE-PEG-Man@EM-NPs) based on cathepsin B-responsive antigenic peptides was designed and synthesized.MethodsHighly affinitive antigenic peptides were delivered by self-assembled NPs, and targeted erythrocyte membranes acted as a peptide carrier to improve antigenic peptides presentation and to strengthen cytotoxic T-cells reaction. Cathepsin B coupling could release antigenic peptides rapidly in dendritic cells.ResultsEvaluations showed that DSPE-PEG-Man@EM-NPs had obvious inhibitory effects towards both MCF-7 and MDA-MB-231 human breast cancer cell lines.ConclusionOverall, this strategy provides a novel strategy for boosting cytotoxic T lymphocytes response, thereby expanding the adaptation range of tumor antigenic peptides and improving the therapeutic effect of tumor immunotherapy with nanomedicine.

Development of novel self‐assembled vaccines based on tumour‐specific antigenic peptide and TLR2 agonist for effective breast cancer immunotherapy via activating CD8+ T cells and enhancing their function

“…The experiment was carried out according to the instructions of the CytoTox 96 ® Non-Radioactive Cytotoxicity Assay (Promega). The cell killing rate was calculated accordingly [30].…”

Self-assembled nanoparticles: A new platform for revolutionizing therapeutic cancer vaccines

“…Wei et al. developed a self-assembled peptide system as a novel adjuvant that can efficiently deliver two antigens (MAGE/NY-ESO-1) ( 86 ). The SANPs not only widened the response range within the same molecule but also significantly prolonged the plasma half-life of single antigenic peptides, which can be a broad-spectrum candidate for effective breast cancer therapy.…”

Combination of Nanomaterials in Cell-Based Drug Delivery Systems for Cancer Treatment

“…Nevertheless, it was found that CD58 and CD59 molecules expressed on erythrocytes membranes could enhance immune recognition and antigen presentation [91]. Shi et al fabricated a modified erythrocytes membrane-cloaked delivery vector (DSPE-PEG-Man@EM-NPs) with shell-core structures for antigen pep-tides delivery towards DCs [39]. The outer layer was an erythrocytes membrane, with DSPE-PEG-mannose decoration that could allow the DC targeting ability, and the inner core was a kind of self-assembly NP, based on amphiphilic cathepsin B-responsive dipeptide to covalently link antigenic peptides MAGE and NY-ESO-1.…”