Design, Synthesis, and Characterization of Graphene–Nanoparticle Hybrid Materials for Bioapplications

Yin, Perry T.; Shah, Shreyas; Chhowalla, Manish; Lee, Ki‐Bum

doi:10.1021/cr500537t

Cited by 647 publications

(419 citation statements)

References 345 publications

Supporting

Mentioning

395

Contrasting

Unclassified

Order By: Relevance

“…field-effect transistor | Debye screening | surface modification | DNA aptamer receptor | polyethylene glycol N anoelectronic biosensors offer broad capabilities for label-free high-sensitivity real-time detection of biological species that are important to both fundamental research and biomedical applications (1)(2)(3)(4)(5)(6). In particular, field-effect transistor (FET) biosensors configured from semiconducting nanowires (1,2), single-walled carbon nanotubes (1,3,4), and graphene (1,5,6) have been extensively investigated since the first report of real-time protein detection using silicon nanowire devices (7).…”

mentioning

confidence: 99%

“…In particular, field-effect transistor (FET) biosensors configured from semiconducting nanowires (1,2), single-walled carbon nanotubes (1,3,4), and graphene (1,5,6) have been extensively investigated since the first report of real-time protein detection using silicon nanowire devices (7). Subsequent studies have demonstrated highly sensitive and in some cases multiplexed detection of key analytes, including protein disease markers (8-10), nucleic acids (11)(12)(13), and viruses (14), as well as detection of protein-protein interactions (8,(15)(16)(17) and enzymatic activity (8).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Specific detection of biomolecules in physiological solutions using graphene transistor biosensors

Gao

Yang

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

221

236

View full text Add to dashboard Cite

Nanomaterial-based field-effect transistor (FET) sensors are capable of label-free real-time chemical and biological detection with high sensitivity and spatial resolution, although direct measurements in high-ionic-strength physiological solutions remain challenging due to the Debye screening effect. Recently, we demonstrated a general strategy to overcome this challenge by incorporating a biomoleculepermeable polymer layer on the surface of silicon nanowire FET sensors. The permeable polymer layer can increase the effective screening length immediately adjacent to the device surface and thereby enable real-time detection of biomolecules in high-ionic-strength solutions. Here, we describe studies demonstrating both the generality of this concept and application to specific protein detection using graphene FET sensors. Concentration-dependent measurements made with polyethylene glycol (PEG)-modified graphene devices exhibited real-time reversible detection of prostate specific antigen (PSA) from 1 to 1,000 nM in 100 mM phosphate buffer. In addition, comodification of graphene devices with PEG and DNA aptamers yielded specific irreversible binding and detection of PSA in pH 7.4 1x PBS solutions, whereas control experiments with proteins that do not bind to the aptamer showed smaller reversible signals. In addition, the active aptamer receptor of the modified graphene devices could be regenerated to yield multiuse selective PSA sensing under physiological conditions. The current work presents an important concept toward the application of nanomaterial-based FET sensors for biochemical sensing in physiological environments and thus could lead to powerful tools for basic research and healthcare.field-effect transistor | Debye screening | surface modification | DNA aptamer receptor | polyethylene glycol N anoelectronic biosensors offer broad capabilities for label-free high-sensitivity real-time detection of biological species that are important to both fundamental research and biomedical applications (1-6). In particular, field-effect transistor (FET) biosensors configured from semiconducting nanowires (1, 2), single-walled carbon nanotubes (1, 3, 4), and graphene (1, 5, 6) have been extensively investigated since the first report of real-time protein detection using silicon nanowire devices (7). Subsequent studies have demonstrated highly sensitive and in some cases multiplexed detection of key analytes, including protein disease markers (8-10), nucleic acids (11-13), and viruses (14), as well as detection of protein-protein interactions (8,(15)(16)(17) and enzymatic activity (8).The success achieved with nanomaterial-based FET biosensors has been limited primarily to measurements in relatively low-ionicstrength nonphysiological solutions due to the Debye screening length (18,19). In short, the screening length in physiological solutions, <1 nm, reduces the field produced by charged macromolecules at the FET surface and thus makes real-time label-free detection difficult. The first method reported to overcome this ...

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

Specific detection of biomolecules in physiological solutions using graphene transistor biosensors

Gao

Yang

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

221

236

View full text Add to dashboard Cite

show abstract

“…In the standard configuration of a FET, the electric current flows along a semiconductor channel connected to the source and drain electrodes. A third electrode, the gate contact, which is capacitively coupled to the device through a thin dielectric layer (typically SiO 2 ), modulates the conductance between these two electrodes [20,24].…”

Section: Fet-based Immunosensorsmentioning

confidence: 99%

Recent Trends in Field-Effect Transistors-Based Immunosensors

Moraes

Kubota

2016

Chemosensors

View full text Add to dashboard Cite

Abstract:Immunosensors are analytical platforms that detect specific antigen-antibody interactions and play an important role in a wide range of applications in biomedical clinical diagnosis, food safety, and monitoring contaminants in the environment. Field-effect transistors (FET) immunosensors have been developed as promising alternatives to conventional immunoassays, which require complicated processes and long-time data acquisition. The electrical signal of FET-based immunosensors is generated as a result of the antigen-antibody conjugation. FET biosensors present real-time and rapid response, require small sample volume, and exhibit higher sensitivity and selectivity. This review brings an overview on the recent literature of FET-based immunosensors, highlighting a diversity of nanomaterials modified with specific receptors as immunosensing platforms for the ultrasensitive detection of various biomolecules.

show abstract

“…Furthermore, HNCs can be controllably engineered with extremely rich structural and topological diversity, thus exhibiting integrated functionalities as well as novel properties stemming from the efficient electronic communication established between joint material domains. All these prerogatives are prohibited both to traditional colloidal nanocomposites that are constructed by exploiting weak (electrostatic or van der Waals) interactions or bifunctional molecules serving as linking bridges between the involved building blocks (Kamat, 2007;Quarta et al, 2007;Bigall et al, 2012;Tian et al, 2015;Yin et al, 2015) and to their heterocluster analogues (albeit frequently based on much larger nano-/micro-particle units) that are derived by assembly mechanisms relying on weak inter-particle forces, external perturbations, and the guide of templating substrates and interfaces Duguet et al, 2011;Gao and Fang, 2015;Vogel et al, 2015;Yan et al, 2015a,b;Yin et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Colloidal Magnetic Heterostructured Nanocrystals with Asymmetric Topologies: Seeded-Growth Synthetic Routes and Formation Mechanisms

2016

View full text Add to dashboard Cite

Colloidal inorganic nanocrystals (NCs), free-standing crystalline nanostructures generated and processed in solution phase, represent an important class of advanced nanoscale materials owing to the flexibility with which their physical-chemical properties can be controlled through synthetic tailoring of their compositional, structural, and geometric features and the versatility with which they can be integrated in technological fields as diverse as optoelectronics, energy storage/conversion/production, catalysis, and biomedicine. In recent years, building upon mechanistic knowledge acquired on the thermodynamic and kinetic processes that underlie NC evolution in liquid media, synthetic nanochemistry research has made impressive advances, opening new possibilities for the design, creation, and mastering of increasingly complex "colloidal molecules," in which NC modules of different materials are clustered together via solid-state bonding interfaces into free-standing, easily processable multifunctional nanocomposite systems. This review will provide a glimpse into this fast-growing research field by illustrating progress achieved in the wet-chemical development of last-generation breeds of allinorganic heterostructured nanocrystals (HNCs) in asymmetric non-onionlike geometries, inorganic analogues of polyfunctional organic molecules, in which distinct nanoscale crystalline modules are interconnected in heterodimer, hetero-oligomer, and anisotropic multidomain architectures via epitaxial heterointerfaces of limited extension. The focus will be on modular HNCs entailing at least one magnetic material component combined with semiconductors and/or metals, which hold potential for generating enhanced or unconventional magnetic behavior, while offering diversified or even new chemical-physical properties and functional capabilities. The available toolkit of synthetic strategies, all based on the manipulation of seeded-growth techniques, will be described, revisited, and critically interpreted within the framework of the currently understood mechanisms of colloidal heteroepitaxy.

show abstract

Design, Synthesis, and Characterization of Graphene–Nanoparticle Hybrid Materials for Bioapplications

Abstract: Graphical abstract

Cited by 647 publications

References 345 publications

Specific detection of biomolecules in physiological solutions using graphene transistor biosensors

Specific detection of biomolecules in physiological solutions using graphene transistor biosensors

Recent Trends in Field-Effect Transistors-Based Immunosensors

Colloidal Magnetic Heterostructured Nanocrystals with Asymmetric Topologies: Seeded-Growth Synthetic Routes and Formation Mechanisms

Contact Info

Product

Resources

About