Design, synthesis and antibacterial evaluation of a polycationic calix[4]arene derivative alone and in combination with antibiotics

Consoli, Grazia Maria Letizia; Granata, Giuseppe; Picciotto, Roberta; Blanco, Anna Rita; Geraci, Corrada; Marino, Andreana; Nostro, Antonia

doi:10.1039/c7md00527j

Cited by 39 publications

(27 citation statements)

References 25 publications

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“…To investigate the antibacterial activity of the MgONPs and bulk MgO, these materials were diluted in a series of concentrations ranging from 15.625 to 1000 μg/mL (1000, 500, 250, 125, 62.5, 31.25, and 15.625 μg/mL), and a typical microdilution method was performed to determine the MIC and MBC of these chemicals against R. solanacearum ( Consoli et al, 2018 ). The MIC and MBC were determined to indicate the bacteriostatic and bactericidal activity.…”

Section: Methodsmentioning

confidence: 99%

Magnesium Oxide Nanoparticles: Effective Agricultural Antibacterial Agent Against Ralstonia solanacearum

et al. 2018

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Magnesium (Mg) is an essential mineral element for plants and is nontoxic to organisms. In this study, we took advantage of nanotechnologies to systematically investigate the antibacterial mechanisms of magnesium oxide nanoparticles (MgONPs) against the phytopathogen Ralstonia solanacearum (R. solanacearum) in vitro and in vivo for the first time. R. solanacearum has contributed to catastrophic bacterial wilt, which has resulted in the world-wide reduction of tobacco production. The results demonstrated that MgONPs possessed statistically significant concentration-dependent antibacterial activity, and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were measured as 200 and 250 μg/mL, respectively. Additional studies, aimed at understanding the toxicity mechanism of MgONPs, indicated that physical injury occurred to the cell membranes, along with decreased motility and biofilm formation ability of R. solanacearum, due to the direct attachment of MgONPs to the surfaces of the bacterial cells, which was observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Reactive oxygen species (ROS) accumulation could also be an important reason for the antibacterial action, inducing DNA damage. The toxicity assessment assay under greenhouse conditions demonstrated that the MgONPs had exerted a large effect on tobacco bacterial wilt, reducing the bacterial wilt index. Altogether, the results suggest that the development of MgONPs as alternative antibacterial agents will become a new research subject.

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Section: Methodsmentioning

confidence: 99%

Magnesium Oxide Nanoparticles: Effective Agricultural Antibacterial Agent Against Ralstonia solanacearum

et al. 2018

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“…Furthermore, the antibacterial activity of MgONFs against Xoo strain GZ 0005 was determined in 96-well microtiter plates by measuring the OD 600 using a microplate reader, which was carried out as described by Consoli et al [25]. In brief, each well of 96-well microtiter plates was filled with 100 μ l solution of MgONFs, which were suspended in NB broth to give a final concentration of 4, 8, and 16 μ g/ml.…”

Section: Methodsmentioning

confidence: 99%

The Green Synthesis of MgO Nano-Flowers Using Rosmarinus officinalis L. (Rosemary) and the Antibacterial Activities against Xanthomonas oryzae pv. oryzae

Abdallah

Ogunyemi

Abdelazez³

et al. 2019

BioMed Research International

121

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Recently, the use of herbs in the agriculture and food industry has increased significantly. In particular, Rosmarinus officinalis L. extracts have been reported to have strong antibacterial properties, which depend on their chemical composition. The present study displayed a biological method for synthesis of magnesium oxide (MgO) nano-flowers. The nano-flowers are developed without using any catalyst agent. Aqueous Rosemary extract was used to synthesize MgO nano-flowers (MgONFs) in stirring conditions and temperature at 70°C for 4 h. The mixture solution was checked by UV-Vis spectrum to confirm the presence of nanoparticles. The MgO nano-flowers powder was further characterized in this study by the X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and Fourier transform infrared spectroscopy. In addition, bacteriological tests indicated that MgO nano-flowers significantly inhibited bacterial growth, biofilm formation, and motility of Xanthomonas oryzae pv. oryzae, which is the causal agent of bacterial blight disease in rice. The electronic microscopic observation showed that bacterial cell death may be mainly due to destroy of cell integrity, resulting in leakage of intracellular content. As recommended, the use of Rosemary extract is an effective and green way to produce the MgO nano-flowers, which can be widely used in agricultural fields to suppress bacterial infection.

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“…An ideal antimicrobial will display specific activity against one species of bacteria, while having no effects on any other organisms, whether they be humans being treated for an infection or the diversity of animal and plant life that may exist in a field where crops are sprayed. An encouraging study into the toxicity and biodistribution of p-sulfonatocalix [4]arene in mice by Coleman and colleagues using a 34 S-labelled derivative found no toxic effects in mice up to 100 mg kg −1 of body weight ( Figure 2) [12]. One animal was given a dose of 400 mg kg −1 and died within an hour, whereas 14 others, receiving doses of 40 or 100 mg kg −1 , were outwardly unaffected.…”

Section: Molecular Prodrugs and Drug Delivery Agentsmentioning

confidence: 99%

“…A polycationic calix [4]arene derivative, 46 ( Figure 9), was prepared by Nostro and colleagues, through the reaction of N-methyldiethanolamine on tetra-propoxy-4-chloromethylcalix [4]arene, to deliver ofloxacin, chloramphenicol, and tetracycline [34]. The calixarene alone had a similar profile to chloramphenicol against S. aureus ATCC 6538, but its MIC was lower by a factor of two against MRSA 15, MRSE 17, and P. aeruginosa isolate 1.…”

Section: Drug-delivering Calixarenesmentioning

confidence: 99%

Antimicrobial Activity of Calixarenes and Related Macrocycles

et al. 2020

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Calixarenes and related macrocycles have been shown to have antimicrobial effects since the 1950s. This review highlights the antimicrobial properties of almost 200 calixarenes, resorcinarenes, and pillararenes acting as prodrugs, drug delivery agents, and inhibitors of biofilm formation. A particularly important development in recent years has been the use of macrocycles with substituents terminating in sugars as biofilm inhibitors through their interactions with lectins. Although many examples exist where calixarenes encapsulate, or incorporate, antimicrobial drugs, one of the main factors to emerge is the ability of functionalized macrocycles to engage in multivalent interactions with proteins, and thus inhibit cellular aggregation.

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Design, synthesis and antibacterial evaluation of a polycationic calix[4]arene derivative alone and in combination with antibiotics

Cited by 39 publications

References 25 publications

Magnesium Oxide Nanoparticles: Effective Agricultural Antibacterial Agent Against Ralstonia solanacearum

Magnesium Oxide Nanoparticles: Effective Agricultural Antibacterial Agent Against Ralstonia solanacearum

The Green Synthesis of MgO Nano-Flowers Using Rosmarinus officinalis L. (Rosemary) and the Antibacterial Activities against Xanthomonas oryzae pv. oryzae

Antimicrobial Activity of Calixarenes and Related Macrocycles

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