“…The solid was filtered. [20,21] To the mixture of benzyl azide (S7, S8) (1.00 mmol, 0.30 g, 0.32 g), respectively, and hex-l-yne (1.1 mmol, 0.09 g), biotin alkyne (1.1 mmol, 0.58 g), propargyl-glycine (1.1 mmol, 0.12 g), respectively in THF/water (1:1) were added CuSO4-5H2O (5 mL, 0.2 mole, 0.04 g) and sodium ascorbate (0.2 mole, 0.03 g) at room temperature. The reaction mixture was stirred at room temperature for 5-6 hours.…”