Design Study for a Cellular Culture Bioreactor Coupled with a Magnetic Stimulation System

Restrepo, Andrés Fernando Mejía; Martínez, Luis Javier; Pinedo, Carlos; Franco, Édinson; Cadavid, Héctor

doi:10.1109/tla.2013.6502791

Cited by 7 publications

(7 citation statements)

References 16 publications

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“…The model verified the magnetic field distribution between the separated coils and their surroundings at different operating conditions, thus verifying the bioreactor and coils system design, and also succeeded in creating a computational model on which to estimate the electrical variables induced under different experimental conditions in vitro (Restrepo et al, 2013). Overall, we obtained a very accurate approximation of the behavior of the magnetic field and how it was acting upon our cellular tissue samples.…”

Section: Discussionsupporting

confidence: 63%

“…Based on the previous approximations modeled in the work by Restrepo et al (2013), we were able to develop and build the a bioreactor design for a Couette-Taylor bioreactor with sufficient processing capacity to allow us to culture cells into biological tissue and allow for the required amounts of growth medium culture within the vessel required for a dermal model, while at the same time having a magnetic stimulation system coupled to its design.…”

Section: Discussionmentioning

confidence: 99%

“…The first equipment basically consists of the source system where the magnitude, frequency and waveform are defined for the generated magnetic field, while the second equipment is the irradiating system where the geometry of the final element is defined for the magnetic field as well as for the positioning of the biological system within the area of interest for the generated field. Both the signal generating and control equipment and the magnetic field generator are described in the work developed by Restrepo et al (2013).…”

Section: Electromagnetic Stimulationmentioning

confidence: 99%

“…We had previously studied the possibility of combining these two lines of research and develop a cellular culture bioreactor coupled with external magnetic stimulation coils in order to study the effects of magnetic field exposure on tissue growth of a skin model, this study was published by Restrepo et al (2013) where the computational model of the integration of these two systems is presented. The main objective is to achieve proper function of the bioreactor for the culture of cellular tissues coupled with an external electromagnetic stimulation system, with the endpoint of recreating an ideal environment, which would allow for the culture and growth of cells in a sterile and favorable environment for the development of tissue grafts exposed to controlled electromagnetic fields.…”

Section: Introductionmentioning

confidence: 99%

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Growth rates of dynamic dermal model exposed to laminar flow and magnetic fields

et al. 2016

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Introduction: Ongoing research in the use of electromagnetic stimulation as coadjuvant in fracture healing has led the authors to begin generating computer models in order to predict cellular growth changes when cells are electromagnetically stimulated. By generating these models, scientists will be able to better understand how electromagnetic fields affect cellular development. The experimental design integrated a cellular culture bioreactor along with an external magnetic stimulation system, which allowed for dermal models to be exposed to controlled magnetic fields. Methods: Initially, it was necessary to analyze the static growth of Normal Human Skin Fibroblast (NHSF) cells when they were exposed to Extremely Low Frequency -Electromagnetic Fields (ELF-EMFs). Using optimal conditions for the NHSF culture, from stimulation signal to scaffolding material, we were able to perform the dynamic flow stimulation experiments. Results: The following systems were developed: (1) a bioreactor aimed at cellular tissue culture, and (2) Helmholtz coils capable of generating stimulation signals for the cultured tissue. The authors were able to appreciate the quantified values of cellular density diluted in all the experiment samples that were taken and overall, the irradiated samples displayed an average increase of 53% higher cellular density for the same amount of initial cellular seeding when the cells were exposed to a 1 mT, 60 Hz magnetic field signal. Conclusion: ELF-EMF's indeed alter NHSF cell growth rates and it is the challenge of the authors to continue investigating what cellular mechanisms are altered when cells are exposed to ELF-EMF's.

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Section: Discussionsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Section: Electromagnetic Stimulationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Growth rates of dynamic dermal model exposed to laminar flow and magnetic fields

et al. 2016

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“…The separation distance h that complies with the Helmholtz condition for the circular coils is equal to its radius a (h = a) [25]. Therefore, the magnetic field along the z axis in Equation (2) can be approximated, by Taylor series representation, as the magnetic field on the segment | z |≤ a/2.…”

Section: B(x Y Z)mentioning

confidence: 99%

Analysis of the Magnetic Field Homogeneity for an Equilateral Triangular Helmholtz Coil

Álvarez¹,

Mejía²,

Cadavid³

et al. 2016

PIER M

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Abstract-This paper presents a mathematical analysis of the magnetic field homogeneity for an Equilateral Triangular Helmholtz (ETH) coil. The magnetic field analysis is based on the Biot-Savart law in which a Taylor series approximation is performed to obtain the analytical distance that complies with the Helmholtz condition between the pair of coils. This is done to compare the magnetic field distributions of the ETH and the Circular Helmholtz (CH) coils for the parameters side length (2a, √ 3a) and radius (a) respectively. Furthermore, an approximate expression of the magnetic field homogeneity with regard to the side length parameter is obtained and finally a computational model of the ETH coil using COMSOL R is performed in order to validate the calculated and experimental results. The results show that the ETH coils have a lower magnetic field homogeneity than the CH coils for the described parameters, and the implementation of either one basically depends on the application specifications.

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Resultados preliminares del crecimiento de tejido celular usando un bioreactor con sistema de estimulación magnética

Restrepo

Martínez

Pinedo

et al. 2014

2014 IEEE Biennial Congress of Argentina (ARGENCON)

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Design Study for a Cellular Culture Bioreactor Coupled with a Magnetic Stimulation System

Cited by 7 publications

References 16 publications

Growth rates of dynamic dermal model exposed to laminar flow and magnetic fields

Growth rates of dynamic dermal model exposed to laminar flow and magnetic fields

Analysis of the Magnetic Field Homogeneity for an Equilateral Triangular Helmholtz Coil

Resultados preliminares del crecimiento de tejido celular usando un bioreactor con sistema de estimulación magnética

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