“…Despite artificial sweeteners (aspartame, saccharin, neotame, sucralose, and acesulfame potassium) have higher sweetness and lower calorie compared to sugar (Schiffman, 2012), yet these compounds are often linked to some diseases due to their side effects, including psychological problems, mental disorder, bladder cancer and heart failure (Masuda and Kitabatake, 2006). Some proteins have been known to elicit the sensation of sweetness (Gnanavel and Muthukumar, 2011;Rega et al, 2015;Masuda and Kitabatake, 2006). These proteins, so-called sweet tasting proteins, include thaumatin, monellin, mabinlin, brazzein, egg lysozyme, and neoculin (previously considered as curculin) (Picone and Temussi, 2012) are considered to be promising as low calorie sweeteners for sugar replacement (Masuda and Kitabatake, 2006).…”