Large‐scale aerial sensing missions can greatly benefit from the perpetual endurance capability provided by high‐performance low‐altitude solar‐powered unmanned aerial vehicles (UAVs). However, today these UAVs suffer from small payload capacity, low energetic margins, and high operational complexity. To tackle these problems, this paper presents four individual technical contributions and integrates them into an existing solar‐powered UAV system: First, a lightweight and power‐efficient day/night‐capable sensing system is discussed. Second, means to optimize the UAV platform to the specific payload and to thereby achieve sufficient energetic margins for day/night flight with payload are presented. Third, existing autonomous launch and landing functionality is extended for solar‐powered UAVs. Fourth, as a main contribution an extended Kalman filter (EKF)‐based autonomous thermal updraft tracking framework is developed. Its novelty is that it allows the end‐to‐end integration of the thermal‐induced roll moment into the estimation process. It is assessed against unscented Kalman filter and particle filter methods in simulation and implemented on the aircraft's low‐power autopilot. The complete system is verified during a 26 h search‐and‐rescue aerial sensing mock‐up mission that represents the first‐ever fully autonomous perpetual endurance flight of a small solar‐powered UAV with a day/night‐capable sensing payload. It also represents the first time that solar‐electric propulsion and autonomous thermal updraft tracking are combined in flight. In contrast to previous work that has focused on the energetic feasibility of perpetual flight, the individual technical contributions of this paper are considered core functionality to guarantee ease‐of‐use, effectivity, and reliability in future multiday aerial sensing operations with small solar‐powered UAVs.