Design of pressure-driven microfluidic networks using electric circuit analogy

Oh, Kwang W.; Lee, Kangsun; Ahn, Byungwook; Furlani, Edward P.

doi:10.1039/c2lc20799k

Cited by 559 publications

(449 citation statements)

References 305 publications

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“…20,23 Thus, researchers have used computational fluid dynamics analysis in microstructure design more frequently to obtain a more accurate and rapid solution. 24,25 In this work, Comsol was used to obtain the optimal hydraulic resistance distribution in the microchannel.…”

Section: B Optimization Of Microchannels and Finite Element Analysismentioning

confidence: 99%

Separation of sperm and epithelial cells based on the hydrodynamic effect for forensic analysis

et al. 2015

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In sexual assault cases, forensic samples are a mixture of sperm from the perpetrator and epithelial cells from the victim. To obtain an independent short tandem repeat (STR) profile of the perpetrator, sperm cells must be separated from the mixture of cells. However, the current method used in crime laboratories, namely, differential extraction, is a time-consuming and labor-intensive process. To achieve a rapid and automated sample pretreatment process, we fabricated a microdevice for hydrodynamic and size-based separation of sperm and epithelial cells. When cells in suspension were introduced into the device's microfluidic channels, they were forced to flow along different streamlines and into different outlets due to their different diameters. With the proposed microdevice, sperm can be separated within a short period of time (0.5 h for a 50-ll mock sample). The STR profiles of the products in the sperm outlet reservoir demonstrated that a highly purified male DNA fraction could be obtained (94.0% male fraction). This microdevice is of low-cost and can be easily integrated with other subsequent analysis units, providing great potential in the process of analyzing sexual assault evidence as well as in other areas requiring cell sorting. V C 2015 AIP Publishing LLC.

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Section: B Optimization Of Microchannels and Finite Element Analysismentioning

confidence: 99%

Separation of sperm and epithelial cells based on the hydrodynamic effect for forensic analysis

et al. 2015

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“…5(a)). 33 To investigate influence of the resistance network on the separation boundary position, we developed CFD-ACEþ models for 1 r/R 100 at Re ¼ 88. We selected Re ¼ 88 since continuous extraction starts to happen in a r/R ¼ 10 device at such Reynolds number (Fig.…”

Section: Optimization Of Outlet Flow Resistancementioning

confidence: 99%

Vortex-aided inertial microfluidic device for continuous particle separation with high size-selectivity, efficiency, and purity

2013

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In this paper, we report an inertial microfluidic device with simple geometry for continuous extraction of large particles with high size-selectivity (<2 lm), high efficiency ($90%), and high purity (>90%). The design takes advantage of a high-aspect-ratio microchannel to inertially equilibrate cells and symmetric chambers for microvortex-aided cell extraction. A side outlet in each chamber continuously siphons larger particles, while the smaller particles or cells exit through the main outlet. The design has several advantages, including simple design, small footprint, ease of paralleling and cascading, one-step operation, and continuous separation with ultra-selectivity, high efficiency and purity. The described approach is applied to manipulating cells and particles for ultraselective separation, quickly and effectively extracting larger sizes from the main flow, with broad applications in cell separations. V C 2013 AIP Publishing LLC.

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“…Firstly, we analogized the complex microfluidic network to an electric circuit. In fluid mechanics, the Hagen-Poiseuille equation is a physical law that accounts for the fluid behavior of pressure-driven flow through a circular channel (Oh et al 2012). In this device, multiple shear stresses can be obtained because of different inlet channels of these chambers, which are treated as resistances in pressure-driven microfluidic networks.…”

Section: Discussionmentioning

confidence: 99%

“…In this device, multiple shear stresses can be obtained because of different inlet channels of these chambers, which are treated as resistances in pressure-driven microfluidic networks. Consequently, by using a physical analogy, complex microfluidic networks could be easily established relatively simple analogy for network-based microdevices (Oh et al 2012). Because it is difficult to measure the fluid flow-induced shear stress value, we performed numerical simulation on the local shear stress distribution in the microchambers.…”

Section: Discussionmentioning

confidence: 99%

“…According to the reference (Oh et al 2012), the hydraulic-electric circuit analogy can be straightforwardly used to prescribe the flow/pressure relation in complex microfluidic networks based on conventional electric circuit theory. Analogous to an electrical circuit, individual channel sections can be treated as resistances within the flow circuit.…”

Section: Numerical Modelingmentioning

confidence: 99%

See 1 more Smart Citation

An integrated microfluidic device for characterizing chondrocyte metabolism in response to distinct levels of fluid flow stimulus

Zhong

Wang

et al. 2013

Microfluid Nanofluid

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In this work, we presented a novel integrated microfluidic perfusion system to generate multiple parameter fluid flow-induced shear stresses simultaneously and investigated the effects of distinct levels of fluid flow stimulus on the responses of chondrocytes, including the changes of morphology and metabolism. Based on the electric circuit analogy, two devices were fabricated, each with four chambers to enable eight different shear stresses spanning over four orders of magnitude from 0.007 to 15.4 dyne/cm 2 with computational fluid dynamics analysis. Chondrocytes subjected to shear stresses (7.5 and 15.4 dyne/cm 2 ) for 24 h reoriented their cytoskeleton to align with the direction of flow. Meanwhile, the collagen I, collagen II and aggrecan expression of chondrocytes increased in different ranges, respectively. Furthermore, interleukin-6 as a proinflammatory cytokine can be detected at shear stress of 7.5 and 15.4 dyne/cm 2 in mRNA level. These results indicated that fluid flow was beneficial for chondrocyte metabolism at interstitial levels (0.007 and 0.046 dyne/cm 2 ), but induced an increase in fibrocartilage phenotype with increasing magnitude of stimulation. Moreover, a moderate level of flow stimulus (7.5 dyne/ cm 2 ) could also result in detrimental cytokine release. This work described a simple and versatile way to rapidly screen cell responses to fluid flow stimulus from interstitial shear stress level to pathological level, providing multi-condition fluid flow-induced microenvironment in vitro for understanding deeply chondrocyte metabolism, cartilage reconstruction and osteoarthritis etiology.

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Design of pressure-driven microfluidic networks using electric circuit analogy

Cited by 559 publications

References 305 publications

Separation of sperm and epithelial cells based on the hydrodynamic effect for forensic analysis

Separation of sperm and epithelial cells based on the hydrodynamic effect for forensic analysis

Vortex-aided inertial microfluidic device for continuous particle separation with high size-selectivity, efficiency, and purity

An integrated microfluidic device for characterizing chondrocyte metabolism in response to distinct levels of fluid flow stimulus

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