Design of optimal solvent for extraction of bio–active ingredients from six varieties of Medicago sativa

Abstract

BackgroundExtensive research has been performed worldwide and important evidences were collected to show the immense potential of plants used in various traditional therapeutic systems. The aim of this work is to investigate the different extracting solvents in terms of the influence of their polarity on the extracting ability of bioactive molecules (phenolic compounds) from the M. sativa flowers.ResultsThe total phenolic content of samples was determined using the Folin Ciocalteu (FC) procedure and their antioxidant activity was assayed through in vitro radical decomposing activity using the radical DPPH° assay (IUPAC name for DPPH is (phenyl)–(2,4,6–trinitrophenyl) iminoazanium). The results showed that water was better than methanol and acetic acid for extracting bioactive compounds, in particular for total phenolic compounds from the flowers of alfalfa. The average content of bioactive molecules in methanol extract was 263.5±1.02 mg GAE/100g of dry weight lyophilized extract. The total phenolic content of the tested plant extracts was highly correlated with the radical decomposing activity. However, all extracts were free–radical inhibitors, but the water extract was more potent than the acetic and the methanol ones. The order of inhibitor effectiveness (expressed by IC50) proved to be: water extract (0.924mg/mL) > acetic acid extract (0.154mg/mL) > methanol (0.079mg/mL). The profiles of each extract (fingerprint) were characterized by FT–MIR spectroscopy.ConclusionsThe present study compares the fingerprint of different extracts of the M. sativa flowers, collected from the wild flora of Romania. The total phenolic content of the tested plant extracts was highly correlated with the radical decomposing activity. The dependence of the extract composition on the solvent polarity (acetic acid vs. methanol vs. water) was revealed by UV–VIS spectrometry and Infrared fingerprint.

Optimization of Ultrasonic-Assisted Extraction of Flavonoid Compounds and Antioxidants from Alfalfa Using Response Surface Method

“…Antioxidant capacity of the extracts is largely dependent on the solvent polarity due to the different potential of compound polarity [9]. In this study, extracts from lower ethanol concentrations appeared higher DPPH (40%, v/v) and ABTS (30%, v/v) radical-scavenging capacities.…”

“…In several studies, different amounts of flavonoids have been found in alfalfa tissues, which indicated that it may be utilized as a potential natural antioxidant compound [9][10][11][12]. Alfalfa may be a promising resource for flavonoids in the future.…”

“…Up to now, several conventional extraction techniques have been used to extract flavonoids like hot water extraction [9], alkaline extraction [13], microwave-assisted extraction [14], supercritical fluid extraction accelerated solvent extraction [15], and ultrasonic-assisted extraction [16,17]. Among these, ultrasonic-assisted extraction has been successful and widely used owing to its high efficiency, time-saving, simplified manipulation, and is environmental-friendly [18,19].…”

Phytochemical Investigation, Gc-MS Profile and Antimicrobial Activity of a Medicinal Plant Ruta Graveolens L. From Ethiopia

“…Polyphenols are liable for antibiotic against microbial agents [24]. [25]. The resistance observed by B. subtillis in methanolic extracts is more than ethanolic extract, while in the case of gram-negative bacteria E. coli was not sensitive to any of the extracts.…”

Multi-response Optimization of Ultrasound-assisted Extraction of Bioactive Components from Medicago Sativa L.

“…It was shown that M. sativa flowers also display notable DPPH radical scavenging activity. [33] Flavonoids are predominantly located in aerial parts of the plants because synthesis of quercetin and other flavonoid derivatives is induced by the sunlight. [3] The role of flavonoids as radical scavengers [34] in the extracts of M. sativa aerial parts is confirmed in this work by the observed negative correlation (P < 0.05) between TF and RSA IC50.…”