Design of metalloenzyme mimics based on self-assembled peptides for organophosphorus pesticides detection

Yang, Yayu; Hao, Sijia; Lei, Xiangmin; Chen, Jianan; Fang, Guozhen; Liu, Jifeng; Wang, Shuo; He, Xingxing

doi:10.1016/j.jhazmat.2022.128262

Cited by 21 publications

(4 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, bared or weak interaction-based probe detection of OPs is not selective enough and requires tedious probe screening, while directly detecting OPs lacks adequate anchoring to the electrodes. Recently, enzyme-mediated sensing methods, which afforded remarkably enhanced selectivity, have shown promising results in OP detections. − Thus, the combination of the single-molecule break junction and enzyme-mediated strategy would achieve high sensitivity and selectivity for the OP detections.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive Detection of Organophosphorus Pesticides Using Single-Molecule Conductance Measurement

et al. 2023

View full text Add to dashboard Cite

Detection of organophosphorus pesticides (OPs) with high sensitivity in environmental samples is of vital importance for environmental safety and human health. However, it remains a challenge to achieve fM (10 −15 mol/L) sensitivity for detecting OPs. Herein, we developed an acetylcholinesterase sensor based on 3,3′,5,5′-tetramethylbenzidine (TMB) combining an enzymemediated strategy and scanning tunneling microscopy break junction (STM-BJ). Benefiting from the enzyme inhibition kinetics of OPs and the customized spectral clustering analysis method, our new strategy achieved the detection of methamidophos (MTMP) with a limit of 10 aM (10 −17 mol/L) and 3 times higher selectivity in mixed OPs. As applied to natural lake waters, it also exhibited high reproducibility, high stability, and good recovery. This work paves a new avenue toward the application of single-molecule conductance characterizations for biochemical analysis and environmental monitoring.

show abstract

Section: Introductionmentioning

confidence: 99%

Ultrasensitive Detection of Organophosphorus Pesticides Using Single-Molecule Conductance Measurement

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Chromatography as a traditional method has been extensively applied to monitor pesticide residues due to its minimal interference, reliable accuracy, and high sensitivity. − However, large-scale equipment with a high cost and sample processing with lengthy times make it difficult to meet the requirement of rapid and repetitive monitoring of the environment and food. − To compensate for these obstacles, electrochemical − and spectroscopic methods ,, have emerged and drawn considerable attention. Fluorescence-based methods, featuring a rapid response, ease of operation, visualization, and high sensitivity, as a noninvasive technology have gathered substantial interest in the monitoring of pesticide residues in agricultural food. ,, …”

Section: Introductionmentioning

confidence: 99%

Temperature-Responsive Covalent Organic Framework-Encapsulated Carbon Dot-Based Sensing Platform for Pyrethroid Detection via Fluorescence Response and Smartphone Readout

Zhang

Zhu

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

In this paper, carbon dot (CD)-encapsulated 1,3,5-tris(4-formylphenyl)benzene (TFB)/2,5-dihydroxyterephthalohydrazide (DHTH) covalent organic frameworks (TDCOFs) grafted with thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) (CDs@TDCOFs@PNIPAM) were fabricated for the detection of pyrethroids. CDs@TDCOFs@PNIPAM achieved a temperatureresponsive "on/off" detection of pyrethroids based on the target-triggered electron-transfer mechanism. The detection limit of pyrethroids was as low as 0.69 μg/L in the wide linear range of 5−400 μg/L (R 2 > 0.9523). Simultaneously, CDs@TDCOFs@ PNIPAM with red, green, and blue (RGB) fluorescence emissions were integrated with a smartphone-assisted device, enabling the visual smart quantitative detection of pyrethroids with a detection limit of 4.875 μg/L. Ultimately, agricultural products were chosen as actual samples to verify the applicability of both recognition modes, and the calculated recovery rate was 105.48−113.40%. Accordingly, CDs@TDCOFs@PNIPAM featuring temperature-responsive switching behavior and RGB fluorescence emission provided a promising analytical strategy for ensuring agricultural and food safety.

show abstract

“…Metalloenzymes usually contain metal ions bound to an amino group of a specific amino acid residue of a peptide or directly to the amide group -carbonyl or nitrogen-offering different coordination environments (Yang, 2022;Martell, 1995;Oloruntoyin, 2022). Thus, a proper de-sign of small molecules with suitable structural and func-tional characteristics could be important for future catalytic, biomimetic and recognition studies.…”

Section: Introductionmentioning

confidence: 99%

Selective union of ATP vs ADP to an amino/amide macrocycle and its Cu2+ complexes: potentiometric, spectrophotometric and theoretical approximations

Serrano

Molina²

2022

J. Eng. Exact Sci.

View full text Add to dashboard Cite

The protonation equilibria of a new amine/amide type macrocycle and its complexation with Cu(II) in solution have been studied by potentiometry and UV-Visible Spectrophotometry. The ligand shows four protonation constants and one unusual deprotonation constant. Six mononuclear and four binuclear copper II complexes were found and reported its stability constant values. Some species predominate in specific ranges of pH, which might be important for biomimetic studies. It was found that the studied ligand forms stable mononuclear and binuclear complexes with Cu (II) and these species have similar geometries, squared pyramid, above pH 7. An evaluation through its potentiometrically determined stability constants of the selective recognition of ATP over ADP by this macrocycle and its Cu (II) complexes is presented. With or without the presence of Cu (II) ions, the ligand forms more stable complexes with ATP over ADP, the stability of these species increase with pH. Both, the stability quotients and the species distribution diagrams for the ATP-ADP mixed systems shows the inclination of the studied systems to selectively bind ATP over ADP, so, in principle, they can be used for the selective recognition of ATP or as catalysts in the hydrolysis of this nucleotide.

show abstract

Design of metalloenzyme mimics based on self-assembled peptides for organophosphorus pesticides detection

Cited by 21 publications

References 59 publications

Ultrasensitive Detection of Organophosphorus Pesticides Using Single-Molecule Conductance Measurement

Ultrasensitive Detection of Organophosphorus Pesticides Using Single-Molecule Conductance Measurement

Temperature-Responsive Covalent Organic Framework-Encapsulated Carbon Dot-Based Sensing Platform for Pyrethroid Detection via Fluorescence Response and Smartphone Readout

Selective union of ATP vs ADP to an amino/amide macrocycle and its Cu2+ complexes: potentiometric, spectrophotometric and theoretical approximations

Contact Info

Product

Resources

About