Design of Intelligent Surface Modifications and Optimal Liquid Handling for Nanoscale Bioanalytical Sensors

Feuz, Laurent; Höök, Fredrik; Reimhult, Erik

doi:10.1002/9781118181249.ch3

Cited by 4 publications

(6 citation statements)

References 156 publications

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“… 4 , 5 For example, assuming the detection limit of 0.1 ng/cm 2 , purely diffusion-based detection of pM-level proteins could take many hours. 6 …”

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confidence: 99%

“…Even with convection-aided analyte transport, it is challenging to detect analytes at sub-10 pM concentrations. 6 …”

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confidence: 99%

“…4,5 For example, assuming the detection limit of 0.1 ng/cm 2 , purely diffusion-based detection of pM-level proteins could take many hours. 6 To overcome this diffusion limit for surface-based biosensors, various schemes have been employed. For example, analyte binding can be accelerated by dispersing nanoparticles in a solution for rapid sampling, 4 by electrokinetic preconcentration of charged molecules toward sensing surfaces, 7 by utilizing evaporation-driven concentration of sample droplets, 8 or by forcing an analyte solution through nanopores to shrink the diffusion distance.…”

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confidence: 99%

“…Even with convection-aided analyte transport, it is challenging to detect analytes at sub-10 pM concentrations. 6 In our experiments, t DEP for each concentration was determined from the time required to achieve signal-to-noise (S/N) ratio of 3. As shown in Figure 5b, we can dramatically change the inverse square dependence in the diffusion-based model to t DEP ∝ C bulk −0.22 using DEP, which allows detection at low concentration within a reasonable time frame.…”

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confidence: 99%

“…With continual improvements in SPR technologies, commercial instruments can detect surface coverage of proteins down to ∼0.1 ng/cm 2 with bulk RI resolution of 10 –7 refractive index units . However, detection of low-concentration analytes depends not only on the instrument itself but also on their transport. , For example, assuming the detection limit of 0.1 ng/cm 2 , purely diffusion-based detection of pM-level proteins could take many hours …”

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confidence: 99%

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Dielectrophoresis-Enhanced Plasmonic Sensing with Gold Nanohole Arrays

et al. 2014

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We experimentally demonstrate dielectrophoretic concentration of biological analytes on the surface of a gold nanohole array, which concurrently acts as a nanoplasmonic sensor and gradient force generator. The combination of nanohole-enhanced dielectrophoresis, electroosmosis, and extraordinary optical transmission through the periodic gold nanohole array enables real-time label-free detection of analyte molecules in a 5 µL droplet using concentrations as low as 1 pM within a few minutes, which is more than 1000 times faster than purely diffusion-based binding. The nanohole-based optofluidic platform demonstrated here is straightforward to construct, applicable to both charged and neutral molecules, and performs a novel function that cannot be accomplished using conventional surface plasmon resonance sensors.

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“… 4 , 5 For example, assuming the detection limit of 0.1 ng/cm 2 , purely diffusion-based detection of pM-level proteins could take many hours. 6 …”

mentioning

confidence: 99%

“…Even with convection-aided analyte transport, it is challenging to detect analytes at sub-10 pM concentrations. 6 …”

mentioning

confidence: 99%