Dielectrophoresis-Enhanced Plasmonic Sensing with Gold Nanohole Arrays

Barik, Avijit; Otto, Lauren M.; Yoo, Daehan; Jose, J.; Johnson, Timothy W.; Oh, Sang Hyun

doi:10.1021/nl500149h

Cited by 161 publications

(158 citation statements)

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“…In addition, a large-area golden nanohole arrays integrated with conductive glass (indium tin oxide: ITO) were also used to demonstrate the dielectrophoresis (DEP)-enhanced SPR sensing. Governed molecules diffuse to the sensor and are significantly accelerated by using applied AC electric field forces on the BSA molecules, as shown in Figure 4 [48]. It is found that the fabricated nanostructure enables label-free and real-time detection of target molecules in the concentrations as low as 1 pM in very short time.…”

Section: Pef From Nanohole Array Substratementioning

confidence: 97%

“…Governed molecules diffuse to the sensor and are significantly accelerated by using applied AC electric field forces on the BSA molecules, as shown in Figure 4. [48] It is found that the a result, the investigation of enhanced fluorescence effect of many typical nonperodical nanostructure, such as silver island film (SiF), fractal-like substrate, on fluorescence have been widely performed.…”

Section: Pef From Nonperiodical Metallic Plasmonic Nanostructurementioning

confidence: 99%

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Recent Progress on Plasmon-Enhanced Fluorescence

et al. 2015

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Abstract:The optically generated collective electron density waves on metal-dielectric boundaries known as surface plasmons have been of great scientific interest since their discovery. Being electromagnetic waves on gold or silver nanoparticle's surface, localised surface plasmons (LSP) can strongly enhance the electromagnetic field. These strong electromagnetic fields near the metal surfaces have been used in various applications like surface enhanced spectroscopy (SES), plasmonic lithography, plasmonic trapping of particles, and plasmonic catalysis. Resonant coupling of LSPs to fluorophore can strongly enhance the emission intensity, the angular distribution, and the polarisation of the emitted radiation and even the speed of radiative decay, which is so-called plasmon enhanced fluorescence (PEF). As a result, more and more reports on surface-enhanced fluorescence have appeared, such as SPASER-s, plasmon assisted lasing, single molecule fluorescence measurements, surface plasmoncoupled emission (SPCE) in biological sensing, optical orbit designs etc. In this review, we focus on recent advanced reports on plasmon-enhanced fluorescence (PEF). First, the mechanism of PEF and early results of enhanced fluorescence observed by metal nanostructure will be introduced. Then, the enhanced substrates, including periodical and nonperiodical nanostructure, will be discussed and the most important factor of the spacer between molecule and surface and wavelength dependence on PEF is demonstrated. Finally, the recent progress of tipenhanced fluorescence and PEF from the rare-earth doped up-conversion (UC) and down-conversion (DC) nanoparticles (NPs) are also commented upon. This review provides an introduction to fundamentals of PEF, illustrates the current progress in the design of metallic nanostructures for efficient fluorescence signal amplification that utilises propagating and localised surface plasmons.

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Section: Pef From Nanohole Array Substratementioning

confidence: 97%

Section: Pef From Nonperiodical Metallic Plasmonic Nanostructurementioning

confidence: 99%

Recent Progress on Plasmon-Enhanced Fluorescence

et al. 2015

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“…The limit of detection far exceeds previously reported label-free optical sensing-based results and can be further improved by using molecule-concentrating techniques and post-measurement signal processing. [54][55][56] The work presented in this manuscript can directly lead to development of convenient label-free optical sensors for large-scale on-chip screening of pancreatic islets required for islet transplantation therapy. The future direction will also be aimed at improving the specificity of hormone detection using these substrates in media such as blood.…”

Section: Conclusion and Discussionmentioning

confidence: 99%

Surface-Enhanced Raman Spectroscopy-Based Label-Free Insulin Detection at Physiological Concentrations for Analysis of Islet Performance

Cho¹,

Kumar²,

Yang³

et al. 2018

ACS Sens.

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Label-free optical detection of insulin would allow in vitro assessment of pancreatic cell functions in their natural state and expedite diabetes-related clinical research and treatment, however no existing method has met these criteria at physiological concentrations. Using spatially-uniform 3D gold-nanoparticle sensors, we have demonstrated surface-enhanced Raman sensing of insulin in the secretions from human pancreatic islets under low and high glucose environments without the use of labels such as antibodies or aptamers. Label-free measurements of the islet secretions showed excellent correlation among the ambient glucose levels, secreted insulin concentrations, and measured Raman-emission intensities. When excited at 785 nm, plasmonic hotspots of the densely-arranged 3D gold-nanoparticle pillars as well as strong interaction between sulphide linkages of the insulin molecules and the gold nanoparticles produced highly sensitive and reliable insulin measurements down to 100 pM. The sensors exhibited a dynamic range of 100 pM to 50 nM with an estimated detection limit of 35 pM, which covers the reported concentration range of insulin observed in pancreatic cell secretions.The sensitivity of this approach is approximately four orders of magnitude greater than previously reported results using label-free optical approaches, and it is much more costeffective than immunoassay-based insulin detection widely used in clinics and laboratories. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 3 Hormones are chemical messengers that control a wide variety of functions in the human body. Maintaining adequate hormone levels is extremely important for human health and disruption to these levels can result in life-debilitating conditions. Simple and easy measurements of hormonal secretions ex vivo or in vivo are essential for implementing next generation biosensors, allowing convenient monitoring of health and early disease detection.One of the most prevalent diseases resulting from hormonal dysfunction is diabetes, which arises from a disruption in the release of insulin in the body. 1-2 Insulin is a peptide hormone which is secreted by beta cells, one of five primary cell-types which populate pancreatic cellular clusters known as islets. The concentration of insulin secreted from beta cells in plasma has been reported to vary between 100 pM (fasting) and 2 nM (about 1 hour after glucose intake) in non-diabetic individuals. [3][4] In diabetic individuals, functional damage to the beta cells reduces or inhibits their ability to release insulin. One of the leading methodologies for treatment of type-1 diabetes is pancreatic islet transplantation, where healthy islets harvested from deceased donors are transplanted into diabetic patients. [5][6] Since the number of donors is limited, methodologies that can improve the efficiency and succe...

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“…However, their sensing capability is generally limited by the relatively low sensitivity, broad spectral linewidth and weak resonance intensity [10][11]. The reported refractive index (RI) sensitivities (detection limits) for these nanoplasmonic sensors are one to two orders of magnitude lower (larger) than those of typical prism-based sensing systems [12][13]. Another widely used nanoplasmonic sensing technique is SPP interferometry, which uses the phase-sensitive interference to control the linewidth of the interference pattern, which may improve the sensing resolution [14][15][16].…”

Section: Introductionmentioning

confidence: 99%