Design of DOX-GNRs-PNIPAM@PEG-PLA Micelle With Temperature and Light Dual-Function for Potent Melanoma Therapy

Abstract

Objective: The aim of this study was to construct light and temperature dual-sensitive micellar carriers loaded with doxorubicin (DOX) and gold nanorods (DOX-GNRs-PNIPAM@PEG-PLA, DAPP) for melanoma therapy.Methods: The DAPP self-assembled using fine-tuned physicochemical properties in water. The DAPP structure, temperature- and photo-sensitivity, drug-release, in-vitro serum stability, and cytotoxicity against melanoma B16F10 cells were evaluated in detail. The corresponding in-vitro and in-vivo therapeutic mechanisms were then evaluated using a B16F10-melanoma bearing BALB/c nude mouse model (B16F10).Results: The light and temperature sensitive micellar drug-delivery system assembled from block copolymer and gold nanorods exhibited a narrow particle size and size distribution, good biocompatibility, well-designed photo-temperature conversion, controlled drug release, and high serum stability. Compared with the free DOX- and PBS-treated groups, the cell endocytosis-mediated cytotoxicity and intra-tumor accumulation of DAPP was markedly enhanced by the NIR-light exposure and induced potent in-vivo tumor inhibitory activity.Conclusion: The design of DAPP, a dual-functional micellar drug-delivery system with temperature- and light-sensitive properties, offers a new strategy for skin-cancer therapy with a potent therapeutic index.

In situ formation of alginic acid‐gold nanohybrids for application in cancer photothermal therapy

“…Despite this size value not being within the size range normally considered optimal (100-200 nm), it is still within the range suitable for intravenous administration, circulation in the human body, and accumulation in the tumor tissue. [5,[35][36][37] The difference in TEM and DLS measurements can be attributed to the sample's physical state in each technique, dried preparation for TEM analysis commonly results in smaller nanoparticles sizes. [38] Additionally, the surface charge measurements showed that the Au-Alg-NHs have a negative value of −25 ± 4.5 mV, which is in accordance with the negative charge of Alg (Figure 2C).…”

Poly(N-isopropylacrylamide)-Based Hydrogels for Biomedical Applications: A Review of the State-of-the-Art

“…Accordingly, systems such as, PNIPAM@ magnetic NPs (Fe 3 O 4 )/5-fluorouracil (5FU) and oxaliplatin (OXA)- acrylic acid (AA), 3-butenoic acid (3BA) or allylamine (AL) (pNIPAM@ Fe 3 O 4 /5FU/OXA-AA, 3BA, AL) [ 181 ], doxorubicin(DOX)-gold nanorods (GNRs)-PNIPAM@poly (d, l-lactide)-poly (ethylene glycol) (PLA-PEG) (DAPP)micelles (DOX-GNRs-PNIPAM@PEG-PLA,DAPP) [ 182 ], vancomycin (VANCO)/SF-Na Alg NPs/PNIPAM@epidermal growth factor (EGF) (VANCO/SF-Na Alg NPs/PNIPAM@EGF) [ 183 ], PNIPAM- superparamagnetic iron oxide nanoparticles (SPION)- etoposide (SPION-PNIPAM-Etoposide) [ 184 ], NaALG-g-P(NIPAM 80 - co -NtBAM 20 )/Dulbecco’s modified Eagle’s medium (DMEM) (NaALG-g-P(NIPAM 80 - co -NtBAM 20 )/DMEM) [ 185 ] and many more have been designed by researchers in order to intensify the use of PNIPAM polymer in the field of drug delivery [ 186 , 187 ]. Table 1 depicts the latest investigations of PNIPAM-based hydrogels in drug delivery.…”

Transdermal Delivery of Therapeutic Compounds With Nanotechnological Approaches in Psoriasis

“…The micelle is susceptible to the influence of the in vivo environment like pH, temperature, or reduction–oxidation reactions, which may result in structural deformation and subsequent release of loaded drugs or induction of drug resistance. In accordance with this property, many pH-triggered and thermosensitive micelles have been designed to achieve targeted drug delivery ( Bae et al, 2005 ; Guo et al, 2018 ; Wang N. et al, 2020 ). Therefore, a micelle is more suitable for topical administration or in combination with other polymer materials when controlled drug release is desired ( Wen et al, 2017 ; Chen et al, 2019 ).…”