Design of Bioactive Nano‐Hybrids for Bone Tissue Regeneration

Surface functionalization of tissue culture polystyrene plates with hydroxyapatite under body fluid conditions and its effect on differentiation behaviors of mesenchymal stem cells

“…Simulated body fluid (SBF), a solution with similar inorganic ion concentrations to those of human plasma, and 1.5SBF, a solution with ion concentrations that are 1.5-fold higher than those of SBF, have been used in the biomimetic deposition of HAp on various material surfaces under mild conditions [5][6][7]. Acidic functional groups such as carboxylate groups displayed on substrate surfaces are known to effectively induce the heterogeneous nucleation of HAp in SBFs [8].…”