Design of artificial small regulatory trans-RNA for gene knockdown in Bacillus subtilis

Yin, Guobin; Peng, Anqi; Zhang, Luyao; Wang, Yan; Du, Guocheng; Chen, Jian; Kang, Zhen

doi:10.1016/j.synbio.2022.11.003

Cited by 3 publications

(4 citation statements)

References 56 publications

(83 reference statements)

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“…This artificial sRNA system was also applied in E. coli , demonstrated to have repression efficiency above 80%, which could function without Hfq, causing lower metabolic burden [ 119 ]. sRNAs with arbitrary sequences and fixed secondary structures were also designed by a de novo sRNA design program to match any gene of interest, which was demonstrated to be pretty efficient in down-regulating expression of comER and ftsZ and functioned well in acetoin biosynthesis regulation [ 119 ]. Arbitrary sequence gets rid of traditional fixed sRNA scaffold, making gene regulation by sRNA more customized.…”

Section: Artificial Srna Design and Applicationmentioning

confidence: 99%

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Regulatory RNAs in Bacillus subtilis: A review on regulatory mechanism and applications in synthetic biology

Peng,

Yin,

Zuo

et al. 2024

Synthetic and Systems Biotechnology

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Section: Artificial Srna Design and Applicationmentioning

confidence: 99%

“…To regulate different genes under different situations, sRNAs with different inhibition efficiency could be chosen. Because of its convenience to construct, this method could also be applied to screen genes of interest through high-throughput screening [ 119 ].…”

Section: Artificial Srna Design and Applicationmentioning

confidence: 99%

Regulatory RNAs in Bacillus subtilis: A review on regulatory mechanism and applications in synthetic biology

Peng,

Yin,

Zuo

et al. 2024

Synthetic and Systems Biotechnology

Self Cite

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“…Moreover, traditional antisense RNAs have limited hibiting gene activity [33,35,36]. In contrast, atsRNA-mediated gene silen merous advantages, providing a straightforward and swift approach to the expression of specific genes [34,37,38]. The inhibitory effect of combining atsRNAs with the self-induced Sal system was initially confirmed in E. coli.…”

Section: Construction Of the Atsrnas That Combine With The Self-induc...mentioning

confidence: 99%

“…Moreover, traditional antisense RNAs have limited efficacy in inhibiting gene activity [33,35,36]. In contrast, atsRNA-mediated gene silencing offers numerous advantages, providing a straightforward and swift approach to down-regulate the expression of specific genes [34,37,38].…”

Section: Construction Of the Atsrnas That Combine With The Self-induc...mentioning

confidence: 99%

The Construction of the Self-Induced Sal System and Its Application in Salicylic Acid Production

Jin,

Gao,

Chen

et al. 2023

Molecules

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The design and construction of more complex and delicate genetic control circuits suffer from poor orthogonality in quorum sensing (QS) systems. The Sal system, which relies on salicylic acid as a signaling molecule, is an artificially engineered regulatory system with a structure that differs significantly from that of natural QS signaling molecules. Salicylic acid is an important drug precursor, mainly used in the production of drugs such as aspirin and anti-HIV drugs. However, there have been no reports on the construction of a self-induced Sal system in single cells. In this study, a high-copy plasmid backbone was used to construct the regulatory proteins and a self-induced promoter of salicylic acid in E. coli by adjusting the precise regulation of key gene expression; the sensitivity and induction range of this system were improved. Subsequently, the exogenous gene pchBA was introduced in E. coli to extend the shikimate pathway and synthesize salicylic acid, resulting in the construction of the first complete self-induced Sal system. Finally, the self-induced Sal System was combined with artificial trans-encoded sRNAs (atsRNAs) to repress the growth-essential gene ppc and accumulate the precursor substance PEP, thereby increasing the titer of salicylic acid by 151%. This construction of a self-induced artificial system introduces a new tool for selecting communication tools and induction systems in synthetic biology and metabolic engineering, but also demonstrates a self-inducible pathway design strategy for salicylic acid biosynthesis.

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Production of pyrimidine nucleosides in microbial systems via metabolic engineering: Theoretical analysis research and prospects

Zhang,

Niu,

Liu

et al. 2024

Biotechnology Advances

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Design of artificial small regulatory trans-RNA for gene knockdown in Bacillus subtilis

Cited by 3 publications

References 56 publications

Regulatory RNAs in Bacillus subtilis: A review on regulatory mechanism and applications in synthetic biology

Regulatory RNAs in Bacillus subtilis: A review on regulatory mechanism and applications in synthetic biology

The Construction of the Self-Induced Sal System and Its Application in Salicylic Acid Production

Production of pyrimidine nucleosides in microbial systems via metabolic engineering: Theoretical analysis research and prospects

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