Design of an Aptamer-Amphiphile for the Detection of β-Lactoglobulin on a Liquid Crystal Interface

Clemente, Ana Paula Brumann; Kuang, Huihui; Shabana, Ahmed M.; Labuza, Theodore P.; Kokkoli, Efrosini

doi:10.1021/acs.bioconjchem.9b00412

Cited by 18 publications

(8 citation statements)

References 36 publications

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“…In hypoallergenic formulas, this process for detecting β‐Lg was successfully tested. Furthermore, a highly specific β‐Lg‐23 aptamer binding affinity for β‐Lg was developed through enzyme‐linked apta‐sorbent assay (Clemente et al., 2019). Within 20 min, the proposed bio‐sensor provided successful identification of β‐Lg with a 0.18 μg/ml or 10 nM LOD.…”

Section: Analytical Methods For the Detection Of Wp Allergensmentioning

confidence: 99%

Whey allergens: Influence of nonthermal processing treatments and their detection methods

Khan

Lin

Ahmed

et al. 2021

Comp Rev Food Sci Food Safe

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Whey and its components are recognized as value-added ingredients in infant formulas, beverages, sports nutritious foods, and other food products. Whey offers opportunities for the food industrial sector to develop functional foods with potential health benefits due to its unique physiological and functional attributes. Despite all the above importance, the consumption of whey protein (WP) can trigger hypersensitive reactions and is a constant threat for sensitive individuals. Although avoiding such food products is the most successful approach, there is still a chance of incorrect labeling and cross-contamination during food processing. As whey allergens in food products are cross-reactive, the phenomenon of homologous milk proteins of various species may escalate to a more serious problem. In this review, nonthermal processing technologies used to prevent and eliminate WP allergies are presented and discussed in detail.These processing technologies can either enhance or mitigate the impact of potential allergenicity. Therefore, the development of highly precise analytical technologies to detect and quantify the existence of whey allergens is of considerable importance. The present review is an attempt to cover all the updated approaches used for the detection of whey allergens in processed food products.Immunological and DNA-based assays are generally used for detecting allergenic proteins in processed food products. In addition, mass spectrometry is also employed as a preliminary technique for detection. We also highlighted the latest improvements in allergen detection toward biosensing strategies particularly immunosensors and aptasensors.

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Section: Analytical Methods For the Detection Of Wp Allergensmentioning

confidence: 99%

Whey allergens: Influence of nonthermal processing treatments and their detection methods

Khan

Lin

Ahmed

et al. 2021

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

show abstract

“…Further, highly specific β-LG-23 aptamer binding affinity to β-LG has been reported via enzyme linked apta-sorbent assay [75]. The proposed biosensor offered effective detection of β-LG with a LOD of 0.18 µg/mL or 10 nM within 20 min.…”

Section: Aptasensorsmentioning

confidence: 95%

Nano-Biosensing Platforms for Detection of Cow’s Milk Allergens: An Overview

Nehra

Lettieri

Dilbaghi

et al. 2019

Sensors

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Among prevalent food allergies, cow milk allergy (CMA) is most common and may persist throughout the life. The allergic individuals are exposed to a constant threat due to milk proteins’ presence in uncounted food products like yogurt, cheese, and bakery items. The problem can be more severe due to cross-reactivity of the milk allergens in the food products due to homologous milk proteins of diverse species. This problem can be overcome by proper and reliable food labeling in order to ensure the life quality of allergic persons. Therefore, highly sensitive and accurate analytical techniques should be developed to detect the food allergens. Here, significant research advances in biosensors (specifically immunosensors and aptasensors) are reviewed for detection of the milk allergens. Different allergic proteins of cow milk are described here along with the analytical standard methods for their detection. Additionally, the commercial status of biosensors is also discussed in comparison to conventional techniques like enzyme-linked immunosorbent assay (ELISA). The development of novel biosensing mechanisms/kits for milk allergens detection is imperative from the perspective of enforcement of labeling regulations and directives keeping in view the sensitive individuals.

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“…Based on the sensing interfaces, LC biosensors can be classified into three categories: LC–solid interface [ 62 ], LC–aqueous interface [ 63 ], and LC–droplet interface [ 20 ]. For the three major geometries of LC biosensors, each has its own special advantages in the detection of biomolecules.…”

Section: Geometries Of Lcs In Biosensingmentioning

confidence: 99%

Liquid Crystal Biosensors: Principles, Structure and Applications

Wang

et al. 2022

Biosensors

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Liquid crystals (LCs) have been widely used as sensitive elements to construct LC biosensors based on the principle that specific bonding events between biomolecules can affect the orientation of LC molecules. On the basis of the sensing interface of LC molecules, LC biosensors can be classified into three types: LC–solid interface sensing platforms, LC–aqueous interface sensing platforms, and LC–droplet interface sensing platforms. In addition, as a signal amplification method, the combination of LCs and whispering gallery mode (WGM) optical microcavities can provide higher detection sensitivity due to the extremely high quality factor and the small mode volume of the WGM optical microcavity, which enhances the interaction between the light field and biotargets. In this review, we present an overview of the basic principles, the structure, and the applications of LC biosensors. We discuss the important properties of LC and the principle of LC biosensors. The different geometries of LCs in the biosensing systems as well as their applications in the biological detection are then described. The fabrication and the application of the LC-based WGM microcavity optofluidic sensor in the biological detection are also introduced. Finally, challenges and potential research opportunities in the development of LC-based biosensors are discussed.

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Design of an Aptamer-Amphiphile for the Detection of β-Lactoglobulin on a Liquid Crystal Interface

Cited by 18 publications

References 36 publications

Whey allergens: Influence of nonthermal processing treatments and their detection methods

Whey allergens: Influence of nonthermal processing treatments and their detection methods

Nano-Biosensing Platforms for Detection of Cow’s Milk Allergens: An Overview

Liquid Crystal Biosensors: Principles, Structure and Applications

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