Design of a Novel Two‐Component Hybrid Dermal Scaffold for the Treatment of Pressure Sores

Sharma, Vaibhav; Kohli, Nupur; Moulding, Dale; Afolabi, Halimat; Hook, Lilian; Mason, Chris; García‐Gareta, Elena

doi:10.1002/mabi.201700185

Cited by 17 publications

(19 citation statements)

References 52 publications

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“…Our laboratory has developed a porous, cross-linked fibrin/alginate scaffold for the treatment of acute full thickness skin wounds and is currently in clinical trials. 11 – 14 The scaffold has exhibited promising advantages in terms of retarding degradation and improving cell ingress, thus enhancing tissue regeneration and establishment of a blood supply. 11 , 12 Since angiogenesis is key for successful bone regeneration, 2 , 3 we hypothesized that our pro-angiogenic fibrin/alginate scaffold could be used for bone tissue engineering applications by adding an osteogenic component to the scaffold, such as a calcium-phosphate (CaP).…”

Section: Introductionmentioning

confidence: 99%

Pro-angiogenic and osteogenic composite scaffolds of fibrin, alginate and calcium phosphate for bone tissue engineering

et al. 2021

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Due to the limitations of bone autografts, we aimed to develop new composite biomaterials with pro-angiogenic and osteogenic properties to be used as scaffolds in bone tissue engineering applications. We used a porous, cross-linked and slowly biodegradable fibrin/alginate scaffold originally developed in our laboratory for wound healing, throughout which deposits of calcium phosphate (CaP) were evenly incorporated using an established biomimetic method. Material characterisation revealed the porous nature and confirmed the deposition of CaP precursor phases throughout the scaffolds. MC3T3-E1 cells adhered to the scaffolds, proliferated, migrated and differentiated down the osteogenic pathway during the culture period. Chick chorioallantoic membrane (CAM) assay results showed that the scaffolds were pro-angiogenic and biocompatible. The work presented here gave useful insights into the potential of these pro-angiogenic and osteogenic scaffolds for bone tissue engineering and merits further research in a pre-clinical model prior to its clinical translation.

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Section: Introductionmentioning

confidence: 99%

Pro-angiogenic and osteogenic composite scaffolds of fibrin, alginate and calcium phosphate for bone tissue engineering

et al. 2021

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“…Two different dermal substitutes were used: the commercially available and clinically well-established Integra and Smart Matrix which is manufactured in our laboratory and is currently undergoing clinical testing. 12 – 14 Variables and levels investigated were as follows: (1) cell passage number (5 or 10); (2) cell seeding density (1.25 × 10 5 , 2.5 × 10 5 , or 5 × 10 5 cells in 200 µL), (3) scaffold disc to well plate surface area ratio (1:1 or 1:6); (4) attachment incubation time (3 or 24 h). The rationale for the chosen cell seeding densities was based on our previous and extensive experience with these materials: 12 – 14 the maximum number of cells that can be seeded per 6-mm-diameter disc of material is 5 × 10 5 .…”

Section: Methodsmentioning

confidence: 99%

“…Similarly, we know from experience that the other chosen variables affect the cell seeding efficiency on these scaffolds. 12 – 14 A full factorial experimental design was used (2 × 2 × 2 × 3). A matrix of variables and levels was created ( Figure 1 ) and for each individual set of experimental conditions, three replicates were performed (n = 3).…”

Section: Methodsmentioning

confidence: 99%

“…Primary normal human dermal fibroblasts (pnHDFs) from a single donor were established from routine surgical excision of normal skin, obtained with informed consent and local ethics committee approval. 12 – 14 Cells were cultured in Dulbecco’s modified Eagles’ medium (DMEM, 31885-023, Gibco, UK; Lot 1683048 used throughout this study) supplemented with 10% foetal bovine serum (FBS, 10270-106, Gibco; Lot 41Q3446K used throughout this study), 10 U/mL of penicillin/streptomycin (15140-122, Gibco) and 200 µL of l -glutamine (25030-024, Gibco) at 37°C with 5% CO 2 . Cell media were changed every 3 days and cells were passaged at ~80% confluency.…”

Section: Methodsmentioning

confidence: 99%

“… 9 – 11 Another example is Smart Matrix ® , which is currently under development and is a 3D cross-linked porous matrix of fibrin and alginate. 12 – 14 Fibrin is crucial to the wound healing process as it plays an active role in physiological repair and in the re-infiltration of both cells and blood vessels. 15 , 16 …”

Section: Introductionmentioning

confidence: 99%

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The importance of factorial design in tissue engineering and biomaterials science: Optimisation of cell seeding efficiency on dermal scaffolds as a case study

Levin¹,

Sharma²,

Hook³

et al. 2018

J Tissue Eng

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This article presents a case study to show the usefulness and importance of using factorial design in tissue engineering and biomaterials science. We used a full factorial experimental design (2 × 2 × 2 × 3) to solve a routine query in every biomaterial research project: the optimisation of cell seeding efficiency for pre-clinical in vitro cell studies, the importance of which is often overlooked. In addition, tissue-engineered scaffolds can be cellularised with relevant cell type(s) to form implantable tissue constructs, where the cell seeding method must be reliable and robust. Our results show the complex relationship between cells and scaffolds and suggest that the optimum seeding conditions for each material may be different due to different material properties, and therefore, should be investigated for individual scaffolds. Our factorial experimental design can be easily translated to other cell types and three-dimensional biomaterials, where multiple interacting variables can be thoroughly investigated for better understanding of cell–biomaterial interactions.

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Optical coherence tomography for in vivo longitudinal monitoring of artificial dermal scaffold

et al. 2023

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Objectives: Artificial dermal scaffold (ADS) has undergone rapid development and been increasingly used for treating skin wound in clinics due to its good biocompatibility, controllable degradation, and low risk of disease infection. To obtain good treatment efficacy, ADS needs to be monitored longitudinally during the treatment process. For example, scaffold-tissue fit, cell in-growth, vascular regeneration, and scaffold degradation are the key properties to be inspected. However, to date, there are no effective, real-time, and noninvasive techniques to meet the requirement of the scaffold monitoring above. Materials and Methods: In this study, we propose to use optical coherence tomography (OCT) to monitor ADS in vivo through three-dimensional imaging. A swept source OCT system with a handheld probe was developed for in vivo skin imaging. Moreover, a cell in-growth, vascular regeneration, and scaffold degradation rate (IRDR) was defined with the volume reduction rate of the scaffold's collagen sponge layer. To measure the IRDR, a semiautomatic image segmentation algorithm was designed based on U-Net to segment the collagen sponge layer of the scaffold from OCT images. Results: The results show that the scaffold-tissue fit can be clearly visualized under OCT imaging. The IRDR can be computed based on the volume of the segmented collagen sponge layer. It is observed that the IRDR appeared to a linear function of the time and in addition, the IRDR varied among different skin parts. Conclusion: Overall, it can be concluded that OCT has a good potential to monitor ADS in vivo. This can help guide the clinicians to control the treatment with ADS to improve the therapy.

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Design of a Novel Two‐Component Hybrid Dermal Scaffold for the Treatment of Pressure Sores

Cited by 17 publications

References 52 publications

Pro-angiogenic and osteogenic composite scaffolds of fibrin, alginate and calcium phosphate for bone tissue engineering

Pro-angiogenic and osteogenic composite scaffolds of fibrin, alginate and calcium phosphate for bone tissue engineering

The importance of factorial design in tissue engineering and biomaterials science: Optimisation of cell seeding efficiency on dermal scaffolds as a case study

Optical coherence tomography for in vivo longitudinal monitoring of artificial dermal scaffold

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