Design of a nanocomposite substrate inducing adult stem cell assembly and progression toward an Epiblast-like or Primitive Endoderm-like phenotype via mechanotransduction

Morena, Francesco; Armentano, Ilaria; Montanucci, Pia; Argentati, Chiara; Fortunati, Elena; Montesano, Simona; Bicchi, Ilaria; Pescara, Teresa; Pennoni, Ilaria; Mattioli, Simona; Torre, Luigi; Latterini, Loredana; Emiliani, Carla; Basta, Giuseppe; Calafiore, Riccardo; Kenny, J. M.; Martino, Sabata

doi:10.1016/j.biomaterials.2017.08.015

Cited by 27 publications

(82 citation statements)

References 92 publications

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“…Tissue engineering applies the concepts of engineering and biology to develop scaffold-based systems with the aim of reproducing the structure and the physiological functions of healthy tissues. The use of stem cells and biomaterials allows the generation of ad hoc bio-hybrid systems that represent 3D-models of tissues either for disease modeling (Figure 1) or, after validation, for therapeutic transplantation [14,37,113,114].…”

Section: Ex Vivo Stem Cell-based Systems: Bio-hybrid Models For Tissumentioning

confidence: 99%

“…These scaffold-based platforms rely on the mechanobiology concept by which biomaterials provide the 3D-structure of native tissues and through their chemical/physical properties are able to specifically steer stem cells toward tissue-specific phenotypes [14]. As a matter of fact, the modulation of physical and chemical properties of biomaterials such as architecture, shape, mechanical properties, and surface structure, have been showed to control the biological responses of stem cells (both stemness and differentiation properties) [113,115,116]. Many types of natural or synthetic biomaterials, such as polymers, ceramics, bio-glass, composites, or seldom metals [37,[117][118][119][120][121][122][123], have been combined with different types of stem cells in order to faithfully recapitulate key environmental signals (thus allowing finer cell/tissue models [11,124]) and elucidate complex biological phenomena such as stem cell reprogramming pathways and determination processes [14].…”

Section: Ex Vivo Stem Cell-based Systems: Bio-hybrid Models For Tissumentioning

confidence: 99%

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Harnessing the Potential of Stem Cells for Disease Modeling: Progress and Promises

Argentati

Tortorella

Bazzucchi

et al. 2020

JPM

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Ex vivo cell/tissue-based models are an essential step in the workflow of pathophysiology studies, assay development, disease modeling, drug discovery, and development of personalized therapeutic strategies. For these purposes, both scientific and pharmaceutical research have adopted ex vivo stem cell models because of their better predictive power. As matter of a fact, the advancing in isolation and in vitro expansion protocols for culturing autologous human stem cells, and the standardization of methods for generating patient-derived induced pluripotent stem cells has made feasible to generate and investigate human cellular disease models with even greater speed and efficiency. Furthermore, the potential of stem cells on generating more complex systems, such as scaffold-cell models, organoids, or organ-on-a-chip, allowed to overcome the limitations of the two-dimensional culture systems as well as to better mimic tissues structures and functions. Finally, the advent of genome-editing/gene therapy technologies had a great impact on the generation of more proficient stem cell-disease models and on establishing an effective therapeutic treatment. In this review, we discuss important breakthroughs of stem cell-based models highlighting current directions, advantages, and limitations and point out the need to combine experimental biology with computational tools able to describe complex biological systems and deliver results or predictions in the context of personalized medicine.Since their establishment, cell cultures have been proven to be the first and most powerful tool for the in vitro investigation of cell biology, from basic research to more complex translational approaches [6]. Classical two-dimensional (2D)-cultures usually consist of a unique type of cells (primary or continuous cultures) that grow in tissue culture polystyrene as adherent monolayers or in floating suspension, both in culture media that provide essential nutrients and growth factors. 2D-strategies are widely used to obtain a large number of cells, thus allowing the in vitro study of molecular mechanisms and development of metabolic assays [7-9]. However, due to their inability to recreate the in vivo complexity of the biological environment, they are not suitable for accurate disease modeling. These limitations have been overcome by the development of three-dimensional (3D)-cell cultures systems [10]. The rationale design of 3D-cell cultures is to harvest cells in microstructures that resemble tissues or organs shape and organization, thus allowing better cell-to-cell/cell-environment contacts and signaling crosstalk [11][12][13][14][15], essential events for tissues correct development and functioning [14,15]. The 3D-cell culture strategy is articulated in many different methods and techniques that can be grouped in scaffold-based or non-scaffold based platforms, each with particular advantages and disadvantages that make them useful for different applications [10,[16][17][18][19]. 3D-cell culture strategies are supported by the in silico...

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Section: Ex Vivo Stem Cell-based Systems: Bio-hybrid Models For Tissumentioning

confidence: 99%

Section: Ex Vivo Stem Cell-based Systems: Bio-hybrid Models For Tissumentioning

confidence: 99%

Harnessing the Potential of Stem Cells for Disease Modeling: Progress and Promises

Argentati

Tortorella

Bazzucchi

et al. 2020

JPM

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“…To overcome this problem, CNT functionalization is achieved by adsorption, electrostatic interaction, or covalent bonding of different molecules and chemistries [62]. In combination with APs, CNTs were mainly used to modulate the bulk properties of the polymeric matrix, such as thermal, mechanical, and electrical properties [10][11][12][13].…”

Section: Nanofillersmentioning

confidence: 99%

“…Although various polymeric biomaterials have proved to be useful in the regenerative medicine, special attention has been given to aliphatic polyesters (APs) [6]. APs are biodegradable and/or bio-based, and their properties can be modulated both by chemical routes [7][8][9] and by physical means by fabricating nanocomposites [10][11][12][13][14][15]. Despite their potential in various biomedical applications, APs lack surface epitopes, have poor biomechanical properties (e.g., low modulus or brittleness), and longer degradation times, e.g., poly(ε-caprolactone), thereby necessitate the incorporation of additional molecules or nanofillers to modulate these characteristics.…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Nanocomposites Based on Aliphatic Polyesters: Design, Synthesis, and Applications in Regenerative Medicine

et al. 2018

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In the last decade, biopolymer matrices reinforced with nanofillers have attracted great research efforts thanks to the synergistic characteristics derived from the combination of these two components. In this framework, this review focuses on the fundamental principles and recent progress in the field of aliphatic polyester-based nanocomposites for regenerative medicine applications. Traditional and emerging polymer nanocomposites are described in terms of polymer matrix properties and synthesis methods, used nanofillers, and nanocomposite processing and properties. Special attention has been paid to the most recent nanocomposite systems developed by combining alternative copolymerization strategies with specific nanoparticles. Thermal, electrical, biodegradation, and surface properties have been illustrated and correlated with the nanoparticle kind, content, and shape. Finally, cell-polymer (nanocomposite) interactions have been described by reviewing analysis methodologies such as primary and stem cell viability, adhesion, morphology, and differentiation processes.

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“…Commonly, three methods are being applied for producing CNTs the rst one is the chemical vapor deposition, the second one is the laser-ablation and carbon arcdischarge system [12]. A combination of carbon nanostructures and polymer matrices widely studied to adjust the mechanical properties and to extend their applications [13][14]. HDPE exhibits good mechanical behavior includes high strength density ratio, lightweight and chemical resistance, however it shows low hardness with long run use [15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Experimental Design of AL2O3/MWCNT/HDPE Hybrid Nanocomposites for Hip Joint Replacement

Dabees

Kamel

Tirth

et al. 2020

Preprint

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Abstract A hip fracture can be considered a major health issue facing the elderly fraction of humans. The loads exploited by the lower limbs are very acute and severe; in the femur, they can be several folds higher than the whole weight of the body. Mechanical properties like strength and hardness are challenging parameters which control the selection of the joint. In this research work, a modification has been performed by adding multi-layer carbon nanotubes to the High-density polyethylene thermoplastic polymer together with alumina. Prepared material well physiochemically characterized to check the morphology and the structure. Thermal gravimetric analysis (TGA) dedicates that the percentage of crystallization has been increased to 6% after adding Multi-wall carbon nanotubes (MWCNT)to the polymer. According to mechanical properties results, MWCNTs successfully increase in young’s module and hardness of the polymer. Optimization of the materials ration has been done and it has been found that hybrid composite with structure (2.4% AL 2 O 3 and 0.6% MWCNT) obtains high mechanical properties compared to other ratios with 3% MWCNTs and 5% MWCNTs. Also, moisture absorption decreased to 90% at with 5% MWCNT ratio. Experimental results of MTT assay of a normal human epithelial cell line (1- BJ1) over AL 2 O 3 /MWCNT@ HDPE showed a low value of cytotoxic activity, which proves that it is proper for medical use.AL 2 O 3 /MWCNT@HDPE composite showed promising results for further studies of artificial joints.

show abstract

Design of a nanocomposite substrate inducing adult stem cell assembly and progression toward an Epiblast-like or Primitive Endoderm-like phenotype via mechanotransduction

Cited by 27 publications

References 92 publications

Harnessing the Potential of Stem Cells for Disease Modeling: Progress and Promises

Harnessing the Potential of Stem Cells for Disease Modeling: Progress and Promises

Recent Advances in Nanocomposites Based on Aliphatic Polyesters: Design, Synthesis, and Applications in Regenerative Medicine

Experimental Design of AL2O3/MWCNT/HDPE Hybrid Nanocomposites for Hip Joint Replacement

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