Design of a Multilayer Cholesterol Amperometric Biosensorfor Preparation and Use in Flow Systems

Vidal, Juan-Carlos; García-Ruiz, E.; Castillo, Juan-Ramón

doi:10.1002/1521-4109(200103)13:3<229::aid-elan229>3.0.co;2-3

Cited by 39 publications

(17 citation statements)

References 36 publications

(40 reference statements)

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“…An alternative method of determination of cholesterol is amperometric enzyme electrodes which allow rapid, accurate, low-cost and sensitive analysis. 3,12 Various types of amperometric enzyme electrodes have been reported for free cholesterol determination. Vol.…”

Section: Introductionmentioning

confidence: 91%

“…38,50 In cholesterol biosensors, poly(o-phenylenediamine) films were also used for elimination interference and determination of cholesterol in human serum could not be performed by using prepared biosensor. 12,51 However, in the present study, poly(ophenylenediamine) film is composed both permselective membrane and immobilization matrix for enzyme.…”

Section: (1)mentioning

confidence: 99%

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Development of an amperometric enzyme electrode based on poly(o-phenylenediamine) for the determination of total cholesterol in serum

Zeybek¹,

Zeybek²,

Pekmez³

et al. 2012

J. Braz. Chem. Soc.

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Um eletrodo enzimático amperométrico baseado em polímero foi preparado para detecção amperométrica de colesterol livre. Inicialmente, o filme polimérico poli(o-fenilenodiamina) (PoPD) foi eletropolimerizado no meio acetonitrila-água contendo o monômero o-fenilenodiamina (oPD) e ácido canforsulfônico em eletrodo de Pt através da técnica de voltametria cíclica. A enzima oxidase colesterol foi imobilizada sobre a superfície do eletrodo Pt/PoPD. A determinação de colesterol foi realizada por maio do monitoramento da corrente de oxidação de H 2 O 2 produzido enzimaticamente a +0,7 V vs. Ag/AgCl. A concentração da solução tampão, o valor de pH e a temperatura de trabalho otimizados foram 0,05 mol L −1 , 7,5 and 40 °C, respectivamente. O intervalo de trabalho do eletrodo de enzima para colesterol foi 9,8 × 10 −3 -11 µmol L −1 e tempo de resposta 150 s. Os efeitos de possíveis interferências presentes em amostras de soro em resposta ao eletrodo de enzima foram examinados. A determinação do colesterol total em amostras de soro foi realizada usando o eletrodo de enzima Pt/PoPD/ChOx proposto e os resultados foram em bom acordo com os obtidos pelo método espectrofotométrico.A polymer-based amperometric enzyme electrode (Pt/PoPD/ChOx) was prepared for the amperometric detection of free cholesterol. Firstly, poly(o-phenylenediamine) (PoPD) polymer film was prepared in acetonitrile-water medium containing o-phenylenediamine (oPD) monomer and (±)-10-camphorsulfonic acid (HCSA) on Pt electrode by the use of cyclic voltammetry technique. Cholesterol oxidase (ChOx) enzyme was immobilized onto Pt/PoPD electrode surface. The determination of cholesterol was performed via monitoring of the oxidation current of enzymatically produced H 2 O 2 at +0.7 V vs. Ag/AgCl. Optimum buffer concentration, pH and working temperature were found as 0.05 mol L −1 , 7.5 and 40 °C, respectively. The working range of enzyme electrode to cholesterol was 9.8 × 10 −3 -11 µmol L −1 and response time 150 s. The effects of possible interferences present in serum samples on response of enzyme electrode were examined. The determination of total cholesterol in serum samples was performed by using proposed Pt/PoPD/ChOx enzyme electrode and results were in good agreement with those obtained by spectrophotometric method.

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Section: Introductionmentioning

confidence: 91%

Section: (1)mentioning

confidence: 99%

Development of an amperometric enzyme electrode based on poly(o-phenylenediamine) for the determination of total cholesterol in serum

Zeybek¹,

Zeybek²,

Pekmez³

et al. 2012

J. Braz. Chem. Soc.

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“…The detection limit calculated using the standard equation (3s/sensitivity) and was found to be 1 Â 10 À16 M, where s is standard deviation of the electrode without hybridization. 41 The comparison of response characteristics of EPD-PANI-Au nanocomposite with other PANI based nanocomposites reported in literature for nucleic acid detection shows that this sensor exhibits higher linear range, sensitivity and stability (as shown in Table 2). (Fig.…”

Section: Genosensor Response Studiesmentioning

confidence: 99%

One-pot synthesis of a polyaniline–gold nanocomposite and its enhanced electrochemical properties for biosensing applications

et al. 2015

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“…However, the sensing current of DA was higher than that of AA which has a lower oxidized potential. It is because some carbons on the polymer backbone was over-oxidized and transformed into carboxylic groups at a high potential when polymerization (Cosnier, 2003;Vidal et al, 2001). In the literature (Palmisano et al, 1995), the carboxylic groups on the over-oxidized conducting polymer, polypyrrole, were proved by X-ray photoelectron spectroscopy (XPS).…”

Section: The Interferences Effect and Monitoring Of Real Samplementioning

confidence: 99%

Amperometric Enzyme-based Biosensors for Lowering the Interferences

Nien¹,

Chen²,

Ho³

2010

Intelligent and Biosensors

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Design of a Multilayer Cholesterol Amperometric Biosensorfor Preparation and Use in Flow Systems

Cited by 39 publications

References 36 publications

Development of an amperometric enzyme electrode based on poly(o-phenylenediamine) for the determination of total cholesterol in serum

Development of an amperometric enzyme electrode based on poly(o-phenylenediamine) for the determination of total cholesterol in serum

One-pot synthesis of a polyaniline–gold nanocomposite and its enhanced electrochemical properties for biosensing applications

Amperometric Enzyme-based Biosensors for Lowering the Interferences

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