Design nanoprobe based on DNA tetrahedron supported hybridization chain reaction and its application to in situ analysis of bacteria

Tang, Longfei; Yang, Jingyi; Liu, Ziting; Qin, Man; Niu, Lili; Zhang, Juan

doi:10.1016/j.cej.2023.143099

Cited by 10 publications

(2 citation statements)

References 32 publications

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“…However, for the detection of bacterial DNA/RNA, the extraction process is often complex and not easily preserved [86][87][88]. With the advent of nucleic acid aptamer technology, which has the potential to replace traditional protein antibody recognition, the conversion of non-nucleic acid targets (e.g., bacterial cells) to nucleic acid signals can be achieved, allowing for more sensitive and safe detection without contamination by nucleic acid amplification techniques such as HCR, which has excellent potential for development [89][90][91].…”

Section: Hcr Combined With Portable Equipment For Point-of-care Detec...mentioning

confidence: 99%

The Application of Hybridization Chain Reaction in the Detection of Foodborne Pathogens

Zhao,

Guo,

et al. 2023

Foods

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Today, with the globalization of the food trade progressing, food safety continues to warrant widespread attention. Foodborne diseases caused by contaminated food, including foodborne pathogens, seriously threaten public health and the economy. This has led to the development of more sensitive and accurate methods for detecting pathogenic bacteria. Many signal amplification techniques have been used to improve the sensitivity of foodborne pathogen detection. Among them, hybridization chain reaction (HCR), an isothermal nucleic acid hybridization signal amplification technique, has received increasing attention due to its enzyme-free and isothermal characteristics, and pathogenic bacteria detection methods using HCR for signal amplification have experienced rapid development in the last five years. In this review, we first describe the development of detection technologies for food contaminants represented by pathogens and introduce the fundamental principles, classifications, and characteristics of HCR. Furthermore, we highlight the application of various biosensors based on HCR nucleic acid amplification technology in detecting foodborne pathogens. Lastly, we summarize and offer insights into the prospects of HCR technology and its application in pathogen detection.

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Section: Hcr Combined With Portable Equipment For Point-of-care Detec...mentioning

confidence: 99%

The Application of Hybridization Chain Reaction in the Detection of Foodborne Pathogens

Zhao,

Guo,

et al. 2023

Foods

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“…Nucleic acid signal amplification is broadly divided into (a) enzyme-assisted methods, such as rolling circle amplification (RCA), − loop-mediated isothermal amplification (LAMP), , and CRISPR/Cas13a, − in which, despite their high efficiency, are susceptible to proteinases in real samples, and (b) enzyme-free methods, like hybridization chain reaction (HCR) , and catalytic hairpin assembly (CHA), , known for cost-effectiveness, operational simplicity, and high sensitivity. In addition, their programmability and robustness allow elegant molecular assembly for highly efficient all-DNA signal amplification in the field of biological and biomedical analysis.…”

Section: Introductionmentioning

confidence: 99%

Hook-Like DNAzyme-Activated Autocatalytic Biosensor for the Universal Detection of Pathogenic Bacteria

Liu,

Shi,

Wang

et al. 2024

Anal. Chem.

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DNAzyme-based assays have found extensive utility in pathogenic bacteria detection but often suffer from limited sensitivity and specificity. The integration of a signal amplification strategy could address this challenge, while the existing combination methods require extensive modification to accommodate various DNAzymes, limiting the wide-spectrum bacteria detection. We introduced a novel hook-like DNAzyme-activated autocatalytic nucleic acid circuit for universal pathogenic bacteria detection. The hook-like connector DNA was employed to seamlessly integrate the recognition element DNAzyme with the isothermal enzyme-free autocatalytic hybridization chain reaction and catalytic hairpin assembly for robust exponential signal amplification. This innovative autocatalytic circuit substantially amplifies the output signals from the DNAzyme recognition module, effectively overcoming DNAzyme's inherent sensitivity constraints in pathogen identification. The biosensor exhibits a strong linear response within a range of 1.5 × 10 3 to 3.7 × 10 7 CFU/mL, achieving a detection limit of 1.3 × 10 3 CFU/mL. Noted that the sensor's adaptability as a universal detection platform is established by simply modifying the hook-like connector module, enabling the detection of various pathogenic bacteria of considerable public health importance reported by the World Health Organization, including Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Salmonella typhimurium. Additionally, the specificity of DNAzyme in bacterial detection is markedly improved due to the signal amplification process of the autocatalytic circuit. This hooklike DNAzyme-activated autocatalytic platform presents a versatile, sensitive, and specific approach for pathogenic bacteria detection, promising to significantly expand the applications of DNAzyme in bacteria detection.

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Advances in Bioreceptor Layer Engineering in Nanomaterial‐based Sensing of Pseudomonas Aeruginosa and its Metabolites

Lapitan,

Felisilda,

Tiangco

et al. 2024

Chemistry An Asian Journal

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Pseudomonas aeruginosa is an opportunistic pathogen that infects wounds and burns and causes severe infections in humans. The high virulence, the emergence of antibiotic‐resistant strains, and the easy transmissibility of P. aeruginosa necessitate its fast detection and control. The gold standard for detecting P. aeruginosa, the plate culture method, though reliable, takes several days to complete. Therefore, developing accurate, rapid, and easy‐to‐use diagnostic tools for P. aeruginosa is highly desirable. Nanomaterial‐based biosensors are at the forefront of detecting P. aeruginosa and its secondary metabolites. This review summarises the biorecognition elements, biomarkers, immobilisation strategies, and current state‐of‐the‐art biosensors for P. aeruginosa. The review highlights the underlying principles of bioreceptor layer engineering and the design of nanomaterial‐based optical, electrochemical, mass‐based, and thermal biosensors. The advantages and disadvantages of these biosensors and their future point‐of‐care applications are also discussed.

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Design nanoprobe based on DNA tetrahedron supported hybridization chain reaction and its application to in situ analysis of bacteria

Cited by 10 publications

References 32 publications

The Application of Hybridization Chain Reaction in the Detection of Foodborne Pathogens

The Application of Hybridization Chain Reaction in the Detection of Foodborne Pathogens

Hook-Like DNAzyme-Activated Autocatalytic Biosensor for the Universal Detection of Pathogenic Bacteria

Advances in Bioreceptor Layer Engineering in Nanomaterial‐based Sensing of Pseudomonas Aeruginosa and its Metabolites

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