Design expert assisted nanoformulation design for co-delivery of topotecan and thymoquinone: Optimization, in vitro characterization and stability assessment

Verma, Devina; Thakur, Pragya S.; Padhi, Santwana; Khuroo, Tahir; Talegaonkar, Sushama; Iqbal, Zeenat

doi:10.1016/j.molliq.2017.07.002

Cited by 52 publications

(19 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the PVA:PMMA:CPX nanofibers mat a two‐stage release kinetics was observed. First order kinetics signifies that the drug release is concentration dependent seen in most of the hydrophilic drug release from PLGA matrix 21 . On the other hand, Hixson–Crowell model indicates that the drug release from the polymer matrix is a function of cube root of the unreleased part of the drug with time.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, Hixson–Crowell model indicates that the drug release from the polymer matrix is a function of cube root of the unreleased part of the drug with time. This model is seen when loss of the polymer exerts the main effect on drug release 21 . Thus, it was concluded that the monolithic nanofibers mat (PVA:CPX, PMMA:CPX) are desorption limited i.e.…”

Section: Discussionmentioning

confidence: 99%

“…The in vitro release data obtained was fitted into the various mathematical models like zero order (Equation ), first order (Equation ), higuchi (Equation ), and hixon crowell (Equation ) for drug release kinetics and analysis. The following equations were used to determine the drug release kinetics of PVA:CPX, PMMA:CPX, and PVA:PMMA:CPX nanofibers mat 21

normalS = S_{0} - k_{0} normalt

\ln normalS = S_{0} - k_{1} normalt

normalS = k_{d} t_{1 / 2}

S^{1 / 3} = {normalS}_{0}^{1 / 3} = k_{t}

where, S is the drug amount (%) released at time t, S 0 is the drug amount present initially at t = 0, k 0 and k 1 are the rate constants for zero and first order reaction, k d is the rate constant for diffusion.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

PVA/PMMA polymer blended composite electrospun nanofibers mat and their potential use as an anti‐biofilm product

Singh

Chauhan

Das

et al. 2020

J of Applied Polymer Sci

Self Cite

View full text Add to dashboard Cite

Bacterial infections have increased dramatically due to microbial biofilm formation resulting in chronic pathological conditions in human subjects. Microbial biofilm causes poor drug penetration and antibiotic resistance, which has made site‐specific sustained drug delivery the most appropriate option. Our work entails fabrication of ciprofloxacin hydrochloride (CPX) loaded nanofibers using polyvinyl alcohol (PVA) and poly(meth) methacrylate (PMMA) employing electrospinning method to form PVA:PMMA:CPX nanofibers mat. These nanofibers mat were optimized, characterized, and further subjected to anti‐biofilm activity. Microscopic images revealed average nanofibers diameter of 243 ± 80 nm with smooth surface morphology. Analytical graphs and thermal analysis confirmed drug encapsulation and drug‐polymer compatibility. In vitro studies demonstrated sustained release of CPX for 22 days displaying Hixon Crowell and two‐stage desorption kinetics. Anti‐biofilm activity showed zones of inhibition 3.0 ± 0.5 cm, 2.8 ± 0.1 cm, 2.9 ± 0.2 cm for Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, respectively which was well above the minimum inhibitory concentration levels of the bacteria forming biofilm. Conclusively, these nanofibers mat have potential to be used as an anti‐biofilm product.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

normalS = S_{0} - k_{0} normalt

\ln normalS = S_{0} - k_{1} normalt

normalS = k_{d} t_{1 / 2}

S^{1 / 3} = {normalS}_{0}^{1 / 3} = k_{t}

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

PVA/PMMA polymer blended composite electrospun nanofibers mat and their potential use as an anti‐biofilm product

Singh

Chauhan

Das

et al. 2020

J of Applied Polymer Sci

Self Cite

View full text Add to dashboard Cite

show abstract

“…A previous study on clobetasol propionate-loaded lecithin-chitosan NPs demonstrated the encapsulation and accumulation in vitro was dependent on the positive charge of polymer [30]. Further studies using the same method have shown less satisfactory results of drug incorporation.…”

Section: Discussionmentioning

confidence: 99%

Self-Assembled Benznidazole-Loaded Cationic Nanoparticles Containing Cholesterol/Sialic Acid: Physicochemical Properties, In Vitro Drug Release and In Vitro Anticancer Efficacy

Santos-Silva

Caland

Nascimento

et al. 2019

IJMS

View full text Add to dashboard Cite

Cationic polymeric nanoparticles (NPs) have the ability to overcome biological membranes, leading to improved efficacy of anticancer drugs. The modulation of the particle-cell interaction is desired to control this effect and avoid toxicity to normal cells. In this study, we explored the surface functionalization of cationic polymethylmethacrylate (PMMA) NPs with two natural compounds, sialic acid (SA) and cholesterol (Chol). The performance of benznidazole (BNZ) was assessed in vitro in the normal renal cell line (HEK-293) and three human cancer cell lines, as follows: human colorectal cancer (HT-29), human cervical carcinoma (HeLa), and human hepatocyte carcinoma (HepG2). The structural properties and feasibility of NPs were evaluated and the changes induced by SA and Chol were determined by using multiple analytical approaches. Small (<200 nm) spherical NPs, with a narrow size distribution and high drug-loading efficiency were prepared by using a simple and reproducible emulsification solvent evaporation method. The drug interactions in the different self-assembled NPs were assessed by using Fourier transform-infrared spectroscopy. All formulations exhibited a slow drug-release profile and physical stability for more than 6 weeks. Both SA and Chol changed the kinetic properties of NPs and the anticancer efficacy. The feasibility and potential of SA/Chol-functionalized NPs has been demonstrated in vitro in the HEK-293, HepG2, HeLa, and HT-29 cell lines as a promising system for the delivery of BNZ.

show abstract

“…Topotecan and TQ were loaded in poly( d , l -lactide- co -glycolide) (PLGA) nanomatrix where topotecan is dissolved in the inner aqueous phase while TQ is loaded into the organic phase of the double emulsion. This nanomatrix enables co-delivery of topotecan and TQ and leads to enhancement of the anticancer effect of topotecan-TQ formula [ 27 ] ( Figure 4 ).…”

Section: Biological Activities Of Thymoquinone Nanoformulationmentioning

confidence: 99%

Protective Roles of Thymoquinone Nanoformulations: Potential Nanonutraceuticals in Human Diseases

El‐Far

Jaouni

et al. 2018

Nutrients

View full text Add to dashboard Cite

The focus on nanotechnology for improved bioavailability and drug delivery is of increasing importance for control of different human diseases. Therefore, numerous nanoformulations have been developed for the oral bioavailability of different drugs. This review introduces applications of nanomedicine to enhance the biological activities of thymoquinone (TQ) to control different diseases in several in vivo studies as a preliminary investigation for human disease treatment with nano-TQ. Nano-TQ effectively augments the anticancer roles of doxorubicin by upregulation of P53 and downregulation of Bcl2 and potentiates paclitaxel’s apoptosis in MCF-7 breast cancer cells. Moreover, nano-TQ protects against diabetes, inflammation, CNS, and hepatotoxicity, mainly by enhancement of organs’ antioxidant status. We summarize the pros and cons of several FDA approved nanoparticle-based therapeutics and discuss the roadblocks in clinical translation, along with potential nano-TQ strategies to overcome these roadblocks. From this review, we can conclude that nano-TQ may be considered as a promising nutraceutical for human health.

show abstract

Design expert assisted nanoformulation design for co-delivery of topotecan and thymoquinone: Optimization, in vitro characterization and stability assessment

Cited by 52 publications

References 43 publications

PVA/PMMA polymer blended composite electrospun nanofibers mat and their potential use as an anti‐biofilm product

PVA/PMMA polymer blended composite electrospun nanofibers mat and their potential use as an anti‐biofilm product

Self-Assembled Benznidazole-Loaded Cationic Nanoparticles Containing Cholesterol/Sialic Acid: Physicochemical Properties, In Vitro Drug Release and In Vitro Anticancer Efficacy

Protective Roles of Thymoquinone Nanoformulations: Potential Nanonutraceuticals in Human Diseases

Contact Info

Product

Resources

About