2019
DOI: 10.3390/ijms20092350
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Self-Assembled Benznidazole-Loaded Cationic Nanoparticles Containing Cholesterol/Sialic Acid: Physicochemical Properties, In Vitro Drug Release and In Vitro Anticancer Efficacy

Abstract: Cationic polymeric nanoparticles (NPs) have the ability to overcome biological membranes, leading to improved efficacy of anticancer drugs. The modulation of the particle-cell interaction is desired to control this effect and avoid toxicity to normal cells. In this study, we explored the surface functionalization of cationic polymethylmethacrylate (PMMA) NPs with two natural compounds, sialic acid (SA) and cholesterol (Chol). The performance of benznidazole (BNZ) was assessed in vitro in the normal renal cell … Show more

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Cited by 16 publications
(14 citation statements)
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“…Extract-loaded nanoparticles (NPEP) were prepared by the nanoprecipitation method [25,26]. The organic phase (OP) (6 mL) containing 45 mg of Eudragit E PO and leaf extract (EP) in 1:10, 1:5, and 2:5 EP/Eudragit E PO ratios (4.5, 9.0, and 13.5 mg, respectively), were injected into the aqueous phase (AP) (14 mL) containing 35 mg of PVA as the surfactant agent under magnetic stirring (720 rpm) at 25 • C [25,26]. For preparation of blank nanoparticles (NPB), 0.75% w/v Eudragit E PO solution in 10:90 v/v (H 2 O/EtOH) was injected into an aqueous solution of 0.25% w/v PVA using a burette at 1.0 mL•min −1 under magnetic stirring at 720 rpm.…”
Section: Preparation Of Extract-loaded Nanoparticlesmentioning
confidence: 99%
See 2 more Smart Citations
“…Extract-loaded nanoparticles (NPEP) were prepared by the nanoprecipitation method [25,26]. The organic phase (OP) (6 mL) containing 45 mg of Eudragit E PO and leaf extract (EP) in 1:10, 1:5, and 2:5 EP/Eudragit E PO ratios (4.5, 9.0, and 13.5 mg, respectively), were injected into the aqueous phase (AP) (14 mL) containing 35 mg of PVA as the surfactant agent under magnetic stirring (720 rpm) at 25 • C [25,26]. For preparation of blank nanoparticles (NPB), 0.75% w/v Eudragit E PO solution in 10:90 v/v (H 2 O/EtOH) was injected into an aqueous solution of 0.25% w/v PVA using a burette at 1.0 mL•min −1 under magnetic stirring at 720 rpm.…”
Section: Preparation Of Extract-loaded Nanoparticlesmentioning
confidence: 99%
“…In this current study, the nanoparticles were prepared by nanoprecipitation method using different extract/Eudragit ratios (Table 3) [43,44]. Among the parameters that are used to characterize nanoparticles, particle size is the most commonly used one, which was the parameter that varied the most according to the different extract/Eudragit ratios [22,26,45,46]. The chromatographic method allowed the proper separation of the different compounds by the reverse-phase chromatography system.…”
Section: Preparation and Characterization Of Extract-loaded Nanoparticles (Npep)mentioning
confidence: 99%
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“…In this era of gene therapy, cationic NPs have gained more interest in cancer research in the past decade [6,101,102]. The most common method of conferring a positive charge on the surface of NPs is by fabricating NPs from multiple components that carry a positive charge at physiological pH.…”
Section: Nanoparticle Design Characteristics To Overcome Barriersmentioning
confidence: 99%
“…Dos Santos-Silva et al [17] studied the effect of surface functionalization with sialic acid (SA) and cholesterol (Chol) on the cellular uptake and in vitro cytotoxicity of benznidazole-loaded cationic polymethylmethacrylate (PMMA) nanoparticles (BNZ NPs). An emulsification solvent evaporation method was used to prepare small (<200 nm) narrow-sized cationic nanoparticles with high drug-loading efficiency.…”
mentioning
confidence: 99%