Design and synthesis of Raman reporter molecules for tissue imaging by immuno‐SERS microscopy

Schütz, Max; Müller, Christian I.; Salehi, Mohammad; Lambert, Christoph; Schlücker, Sebastian

doi:10.1002/jbio.201000116

Cited by 34 publications

(25 citation statements)

References 39 publications

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“…Minimal sampling was suggested for rapid acquisitions of Raman images [104]. Raman signals can be enhanced in tissue or cells by surface enhanced Raman scattering (SERS) effect using functionalized metal nanoparticles [105][106][107] or by resonance Raman scattering probing prosthetic groups in chromophores [108]. Another promising signal enhancement approach constitutes non-linear, coherent Raman techniques such as coherent anti-Stokes Raman scattering (CARS) or stimulated Raman scattering [109].…”

Section: Discussionmentioning

confidence: 99%

Molecular pathology via IR and Raman spectral imaging

Diem¹,

Mazur²,

Lenau³

et al. 2013

Journal of Biophotonics

172

115

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Journal of BIOPHOTONICSDuring the last 15 years, vibrational spectroscopic methods have been developed that can be viewed as molecular pathology methods that depend on sampling the entire genome, proteome and metabolome of cells and tissues, rather than probing for the presence of selected markers. First, this review introduces the background and fundamentals of the spectroscopies underlying the new methodologies, namely infrared and Raman spectroscopy. Then, results are presented in the context of spectral histopathology of tissues for detection of metastases in lymph nodes, squamous cell carcinoma, adenocarcinomas, brain tumors and brain metastases. Results from spectral cytopathology of cells are discussed for screening of oral and cervical mucosa, and circulating tumor cells. It is concluded that infrared and Raman spectroscopy can complement histopathology and reveal information that is available in classical methods only by costly and time-consuming steps such as immunohistochemistry, polymerase chain reaction or gene arrays. Due to the inherent sensitivity toward changes in the bio-molecular composition of different cell and tissue types, vibrational spectroscopy can even provide information that is in some cases superior to that of any one of the conventional techniques.Schematic of spectral histopathology (SHP) process: diagnostic algorithm is developed by spectral data of well documented specimens and subsequently applied to unknown dataset.

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Section: Discussionmentioning

confidence: 99%

Molecular pathology via IR and Raman spectral imaging

Diem¹,

Mazur²,

Lenau³

et al. 2013

Journal of Biophotonics

172

115

View full text Add to dashboard Cite

show abstract

“…A stoichiometric ratio of Ra 1 : Ra 2 ¼ 100 : 1 was chosen to ensure the steric accessibility of the carboxyl groups in the densely packed SAM. 19,20,39 The addition of DTNB-MEG-OH (Ra 1 ) and DTNB-TEG-CO 2 H (Ra 2 ) to the metal colloid leads to a hydrophilically stabilized self-assembled monolayer comprising two different terminal spacer units (dual spacer-SAM, cf. Fig.…”

Section: Characterization Of Antibody-sers Label Conjugatesmentioning

confidence: 99%

Microspectroscopic SERS detection of interleukin-6 with rationally designed gold/silver nanoshells

Wang

Salehi

Schütz

et al. 2013

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Rationally designed gold/silver nanoshells (Au/Ag-NS) with plasmon resonances optimized for red laser excitation in order to minimize autofluorescence from clinical samples exhibit scattering cross-sections, which are ca. one order of magnitude larger compared with solid quasi-spherical gold nanoparticles (Au-NPs) of the same size. Hydrophilic stabilization and sterical accessibility for subsequent bioconjugation of Au/Ag-NS is achieved by coating their surface with a self-assembled monolayer (SAM) of rationally designed Raman reporter molecules comprising terminal mono-and tri-ethylene glycol (EG) spacers, respectively. The stability of the hydrophilically stabilized metal colloid was tested under different conditions. In contrast to metal colloids coated with a SAM without terminal EG spacers, the hydrophilically stabilized SERS particles do not aggregate under physiologically relevant conditions, i.e., buffer solutions with high ionic strength. Using these rationally designed SERS particles in conjunction with a microspectroscopic acquisition scheme, a sandwich immunoassay for the sensitive detection of interleukin-6 (IL-6) was developed. Several control experiments demonstrate the high specificity of the assay towards IL-6, with a lowest detectable concentration of ca. 1 pg mL À1 . The signal strength of the Au/Ag-NS is at least one order of magnitude higher compared with hydrophilically stabilized, non-aggregated solid quasi-spherical Au-NPs of the same size.

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“…The approach may also lack spectral resolution in some applications where characteristic reporter bands (such as the phenyl ring modes of 4-MBA) only exhibit small wavenumber shifts (few cm –1 ) as a function of bound substituents. 155 – 158 …”

Section: Intracellular Sersmentioning

confidence: 99%