Nanoparticles and intracellular applications of surface-enhanced Raman spectroscopy

Taylor, Jack A.; Huefner, Anna; Li, Li; Wingfield, Jonathan; Mahajan, Sumeet

doi:10.1039/c6an01003b

Cited by 89 publications

(40 citation statements)

References 165 publications

(328 reference statements)

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“…Although SERS microspectroscopy has been extensively employed in the study of tumor tissues (this will be discussed extensively in the next section), the phenotypic characterization of single cells is still in its infancy (Altunbek et al 2016;Chourpa et al 2008;Hu et al 2016;Kneipp 2017;Nolan et al 2012;Taylor et al 2016). The rationale of using SERS for single cell studies over other imaging techniques, such as those based on fluorescence read-outs, rests on its high multiplexing capabilities, sensitivity and robustness to investigate the distinct properties of cancer cells, in particular by exploiting antibody-conjugated SEPs targeting cell membrane receptors for immunophenotyping studies.…”

Section: Sep Characterization Of Single Cellsmentioning

confidence: 99%

Cancer characterization and diagnosis with SERS-encoded particles

et al. 2017

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Section: Sep Characterization Of Single Cellsmentioning

confidence: 99%

Cancer characterization and diagnosis with SERS-encoded particles

et al. 2017

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“…These methods include surface enhanced Raman scattering (SERS) that uses noble metal surfaces to enhance the signal up to million folds and coherent Raman scattering that enables fast and improved readout of chemical information from scattering images . SERS is probably more popular of the two for single cell analysis and often plasmonic NPs such as Au have been used as Raman signal enhancers. On the other hand, surface enhanced Raman spectroscopy has also been used to visualize intracellular NPs .…”

Section: Methods For Qualitative and Quantitative Analysis Of Cell‐asmentioning

confidence: 99%

Methodologies and approaches for the analysis of cell–nanoparticle interactions

Ivask

Mitchell

Malysheva

et al. 2017

WIREs Nanomed Nanobiotechnol

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How to study nanoparticle-cell interactions is the key question that puzzles researchers in the fields of nanomedicine as well as in nanotoxicology. In nanotoxicology, the amount of nanoparticles internalized by the cells or bound to the external surfaces of cells determines the toxic profile of those particles. In medical applications, cellular uptake and binding of medically effective nanoparticles decides their efficacy. Despite the importance of understanding the extent and mode of nanoparticle-cell interactions, these processes are underinvestigated, mainly due to the lack of suitable user-friendly methodologies. Here we discuss the advantages and limitations of currently available (and most advanced) microscopic, spectroscopic, and other bioanalytical methods that could be used to assess cell-nanoparticle interactions either qualitatively or quantitatively. Special emphasis is given to the methods that enable analysis and identification of nanoparticles at single-cell level, and allow intracellular localization and speciation analysis of nanoparticles. This article is categorized under: Nanotechnology Approaches to Biology > Cells at the Nanoscale Toxicology and Regulatory Issues in Nanomedicine > Toxicology of Nanomaterials.

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“…It is often difficult using existing techniques to characterize the properties of ENMs within foods because food matrices have such complex compositions and structures, and complete characterization of ENMs in food matrices may require development of new methods (Bellmann et al , 2015; Szakal et al , 2014a). Other analytical tools that might be used to provide valuable information about nanoparticle characteristics in the complex milieu of the GIT include Surface-enhanced Raman spectroscopy (SERS) (Taylor et al , 2016), single particle inductively coupled plasma mass spectrometry (sp-ICP-MS) (Ma et al , 2016; Montano et al , 2016), Field flow fractionation (FFF) (Laborda et al , 2016; Zattoni et al , 2014), X-ray absorption near edge structure (XANES) (Grafe et al , 2014; Kuzmin and Chaboy, 2014) and scanning and transmission electron microscopy (SEM and TEM).…”

Section: Ienm Property Transformations: Impact Of Food Matrix Effectsmentioning

confidence: 99%

The role of the food matrix and gastrointestinal tract in the assessment of biological properties of ingested engineered nanomaterials (iENMs): State of the science and knowledge gaps

et al. 2016

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Many foods contain appreciable levels of engineered nanomaterials (ENMs) (diameter < 100 nm) that may be either intentionally or unintentionally added. These ENMs vary considerably in their compositions, dimensions, morphologies, physicochemical properties, and biological responses. From a toxicological point of view, it is often convenient to classify ingested ENMs (iENMs) as being either inorganic (such as TiO2, SiO2, Fe2O3, or Ag) or organic (such as lipid, protein, or carbohydrate), since the former tend to be indigestible and the latter are generally digestible. At present there is a relatively poor understanding of how different types of iENMs behave within the human gastrointestinal tract (GIT), and how the food matrix and biopolymers transform their physico-chemical properties and influence their gastrointestinal fate. This lack of knowledge confounds an understanding of their potential harmful effects on human health. The purpose of this article is to review our current understanding of the GIT fate of iENMs, and to highlight gaps where further research is urgently needed in assessing potential risks and toxicological implications of iENMs. In particular, a strong emphasis is given to the development of standardized screening methods that can be used to rapidly and accurately assess the toxicological properties of iENMs.

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Nanoparticles and intracellular applications of surface-enhanced Raman spectroscopy

Abstract: Surface-enhanced Raman spectroscopy offers ultra-sensitive vibrational fingerprinting within biological cells.

Cited by 89 publications

References 165 publications

Cancer characterization and diagnosis with SERS-encoded particles

Cancer characterization and diagnosis with SERS-encoded particles

Methodologies and approaches for the analysis of cell–nanoparticle interactions

The role of the food matrix and gastrointestinal tract in the assessment of biological properties of ingested engineered nanomaterials (iENMs): State of the science and knowledge gaps

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