“…Examples include several reporter constructs (e.g. GFP, sfGFP, mCherry, mSCFP3, and LacZ, worked nicely, whereas functionality of RFP, YFP, and eBFP2 was poor) as well as proteins, that are largely insoluble, typically yielded at low concentrations in E. coli, isotopically labeled proteins or even multimeric membrane protein complexes [6,7,20,28,31,[36][37][38].…”