Design and optimization of a fused‐silica microfluidic device for separation of trivalent lanthanides by isotachophoresis

Bottenus, Danny; Branch, Shirmir D.; Lackey, Hope E.; Ivory, Cornelius F.; Katalenich, Jeff; Clark, Sue; Lines, Amanda M.

doi:10.1002/elps.201900027

Cited by 5 publications

(5 citation statements)

References 70 publications

(99 reference statements)

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“…Plateau-mode ITP was among the earliest forms of ITP separations, starting at least as far back as the 1970s, and has been extensively used in capillary systems. ,, This approach has also been implemented in microfluidic ITP separation processes. For example, Cong et al and later Bottenus et al (cf. Figure ) demonstrated the separation of lanthanides in microfluidic devices.…”

Section: Applications Of Microfluidic Itpmentioning

confidence: 99%

“…C 4 D modules are now available commercially and are commonly used as detectors for on-chip microfluidic ITP applications. For example, Cong et al 358 and Bottenus et al 359 used commercial C 4 D units to monitor the ITP separation of lanthanides on custom-built PMMA and fused-silica microfluidic devices, respectively. As another example, Koczka et al 186 combined UV detection and C 4 D and performed ITP analyses on a variety of samples such as red wine, fizzy drink, and juice.…”

Section: Conductivity Detectionmentioning

confidence: 99%

“…1 , 168 , 238 This approach has also been implemented in microfluidic ITP separation processes. For example, Cong et al 358 and later Bottenus et al 359 (cf. Figure 30 ) demonstrated the separation of lanthanides in microfluidic devices.…”

Section: Applications Of Microfluidic Itpmentioning

confidence: 99%

Section: Applications Of Microfluidic Itpmentioning

confidence: 99%

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Isotachophoresis: Theory and Microfluidic Applications

Ramachandran

Santiago

2022

Chem. Rev.

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Isotachophoresis (ITP) is a versatile electrophoretic technique that can be used for sample preconcentration, separation, purification, and mixing, and to control and accelerate chemical reactions. Although the basic technique is nearly a century old and widely used, there is a persistent need for an easily approachable, succinct, and rigorous review of ITP theory and analysis. This is important because the interest and adoption of the technique has grown over the last two decades, especially with its implementation in microfluidics and integration with on-chip chemical and biochemical assays. We here provide a review of ITP theory starting from physicochemical first-principles, including conservation of species, conservation of current, approximation of charge neutrality, pH equilibrium of weak electrolytes, and so-called regulating functions that govern transport dynamics, with a strong emphasis on steady and unsteady transport. We combine these generally applicable (to all types of ITP) theoretical discussions with applications of ITP in the field of microfluidic systems, particularly on-chip biochemical analyses. Our discussion includes principles that govern the ITP focusing of weak and strong electrolytes; ITP dynamics in peak and plateau modes; a review of simulation tools, experimental tools, and detection methods; applications of ITP for on-chip separations and trace analyte manipulation; and design considerations and challenges for microfluidic ITP systems. We conclude with remarks on possible future research directions. The intent of this review is to help make ITP analysis and design principles more accessible to the scientific and engineering communities and to provide a rigorous basis for the increased adoption of ITP in microfluidics.

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Section: Applications Of Microfluidic Itpmentioning

confidence: 99%

Section: Conductivity Detectionmentioning

confidence: 99%

Section: Applications Of Microfluidic Itpmentioning

confidence: 99%

Section: Applications Of Microfluidic Itpmentioning

confidence: 99%

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Isotachophoresis: Theory and Microfluidic Applications

Ramachandran

Santiago

2022

Chem. Rev.

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“…We calculated the Mean Percent Error (MPE) from the expected count. This is a commonly used method for calculating percentage error in microfluidic devices 82 – 90 .This method helps quantify the overall trend of variations across multiple replicates, whether they are consistently higher or lower than expected. The resulting MPE provides a measure of the average magnitude and direction of the deviations from the expected counts.…”

Section: Methodsmentioning

confidence: 99%

Counter-on-chip for bacterial cell quantification, growth, and live-dead estimations

Rahman,

Butzin

2024

Sci Rep

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Quantifying bacterial cell numbers is crucial for experimental assessment and reproducibility, but the current technologies have limitations. The commonly used colony forming units (CFU) method causes a time delay in determining the actual numbers. Manual microscope counts are often error-prone for submicron bacteria. Automated systems are costly, require specialized knowledge, and are erroneous when counting smaller bacteria. In this study, we took a different approach by constructing three sequential generations (G1, G2, and G3) of counter-on-chip that accurately and timely count small particles and/or bacterial cells. We employed 2-photon polymerization (2PP) fabrication technology; and optimized the printing and molding process to produce high-quality, reproducible, accurate, and efficient counters. Our straightforward and refined methodology has shown itself to be highly effective in fabricating structures, allowing for the rapid construction of polydimethylsiloxane (PDMS)-based microfluidic devices. The G1 comprises three counting chambers with a depth of 20 µm, which showed accurate counting of 1 µm and 5 µm microbeads. G2 and G3 have eight counting chambers with depths of 20 µm and 5 µm, respectively, and can quickly and precisely count Escherichia coli cells. These systems are reusable, accurate, and easy to use (compared to CFU/ml). The G3 device can give (1) accurate bacterial counts, (2) serve as a growth chamber for bacteria, and (3) allow for live/dead bacterial cell estimates using staining kits or growth assay activities (live imaging, cell tracking, and counting). We made these devices out of necessity; we know no device on the market that encompasses all these features.

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Capacitively coupled contactless conductivity detection for analytical techniques – Developments from 2018 to 2020

Hauser

Kubáň

2020

Journal of Chromatography A

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Design and optimization of a fused‐silica microfluidic device for separation of trivalent lanthanides by isotachophoresis

Cited by 5 publications

References 70 publications

Isotachophoresis: Theory and Microfluidic Applications

Isotachophoresis: Theory and Microfluidic Applications

Counter-on-chip for bacterial cell quantification, growth, and live-dead estimations

Capacitively coupled contactless conductivity detection for analytical techniques – Developments from 2018 to 2020

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