Design and Invitro Characterization of Metformin Loaded Resealed Erythrocytes

Viswanad, Vidya; Aiswarya, S; Parvathy, Subramanian S.; Tp, Aneesh

doi:10.22159/ajpcr.2017.v10i4.16636

Cited by 3 publications

(2 citation statements)

References 10 publications

(10 reference statements)

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“…This loading was higher than most of formerly published results, such as amphotericin B (16%) 27 , ambroxol hydrochloride (61%) 28 , pravastatin chitosan nanogels (36%) 29 , and methotrexate (60%) 30 . On the other hand, 61% berberine loading in endocytosis method was almost comparable with previously published data for pravastatin (94%) 31 , artemether (14%) 32 , metformin (90%) 33 and salbutamol (68%) 34 . As revealed in Fig.…”

Section: Discussionsupporting

confidence: 90%

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Intelligent berberine-loaded erythrocytes attenuated inflammatory cytokine productions in macrophages

Aghili,

Magnani,

Ghatrehsamani

et al. 2024

Sci Rep

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Erythrocytes are impressive tools for drug delivery, especially to macrophages. Therefore, berberine was loaded into erythrocytes using both hypotonic pre-swelling and endocytosis methods to target macrophages. Physicochemical and kinetic parameters of the resulting carrier cells, such as drug loading/release kinetics, osmotic fragility, and hematological indices, were determined. Drug loading was optimized for the study using Taguchi experimental design and lab experiments. Loaded erythrocytes were targeted to macrophages using ZnCl2 and bis-sulfosuccinimidyl-suberate, and targeting was evaluated using flow cytometry and Wright–Giemsa staining. Differentiated macrophages were stimulated with lipopolysaccharide, and the inflammatory profiles of macrophages were evaluated using ELISA, western blotting, and real-time PCR. Findings indicated that the endocytosis method is preferred due to its low impact on the erythrocyte’s structural integrity. Maximum loading achieved (1386.68 ± 22.43 μg/ml) at 1500 μg/ml berberine treatment at 37 °C for 2 h. Berberine successfully inhibited NF-κB translation in macrophages, and inflammatory response markers such as IL-1β, IL-8, IL-23, and TNF-α were decreased by approximately ninefold, sixfold, twofold, eightfold, and twofold, respectively, compared to the LPS-treated macrophages. It was concluded that berberine-loaded erythrocytes can effectively target macrophages and modulate the inflammatory response.

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Section: Discussionsupporting

confidence: 90%

“…Subsequently, erythrocytes pellet were resuspended with a hematocrit of 30% in autologous plasma for 60 min at 37 °C to promote IgG binding. Finally, erythrocytes were washed once in an isotonic salt solution containing 2% (w/v) BSA and rinsed in a washing isotonic salt solution 33 .…”

Section: Methodsmentioning

confidence: 99%

Intelligent berberine-loaded erythrocytes attenuated inflammatory cytokine productions in macrophages

Aghili,

Magnani,

Ghatrehsamani

et al. 2024

Sci Rep

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Erythrocyte alterations in specimens of Danio rerio caused by exposure to metformin

Barbieri,

Lupepsa,

Paupitz

et al. 2024

Environmental Toxicology and Pharmacology

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Impact of Metformin and Serratiopeptidase in Obese Patients With Knee Osteoarthritis

Ateia

Al-Edanni

Al-qurtas

2018

Int J Pharm Pharm Sci

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Objective: Knee osteoarthritis is a common disabling chronic disease globally. Many pharmacological agents have been used efficiently in treatment of knee osteoarthritis. This study aims to evaluate metformin and serratiopeptidase together in treatment and stop of osteoarthritis progression by different mechanisms. Methods:Present study was a randomized clinical trial study conducted in Al-Kindi teaching hospital through the period from 1 st January to 30 th of May, 2017 on two groups of 80 osteoarthritis patients (group I; treated with metformin 850 mg oral tablets) and (group II; treated with metformin 850 mg oral tablets and serratiopeptidase 20 mg oral tablets). Parameters of two groups were compared with those of 40 normal healthy controls. Results:Patients in group II showed a highly significant reduction in pain scores post-treatment (p<0.001). Tumor necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1ß) and interleukin 8 (IL-8) levels were significantly lowered among patients in group II treatment (p<0.001). Lower inflammatory parameters levels were observed among healthy controls and the parameters levels of group II patients were lower than those of group I patients. Conclusion:Metformin and serratiopeptidase regimen was efficient and safe in the treatment of knee osteoarthritis.

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Design and Invitro Characterization of Metformin Loaded Resealed Erythrocytes

Cited by 3 publications

References 10 publications

Intelligent berberine-loaded erythrocytes attenuated inflammatory cytokine productions in macrophages

Intelligent berberine-loaded erythrocytes attenuated inflammatory cytokine productions in macrophages

Erythrocyte alterations in specimens of Danio rerio caused by exposure to metformin

Impact of Metformin and Serratiopeptidase in Obese Patients With Knee Osteoarthritis

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