Design and gene delivery activity of modified polyethylenimines

PEI/DNA formation affects transient gene expression in suspension Chinese hamster ovary cells via a one-step transfection process

“…Apart from the size of the complex, the overall charge of PEI/DNA particles is another parameter that affects transfection efficiency. Moreover, different PEI/DNA ratios could change the complex charge (Davis et al 1993;Kircheis et al 2001;Backliwal et al 2008). The interaction and stability of all PEI/DNA complexes were confirmed by gel retardation assay.…”

“…2). Previous research has suggested that compact PEI/DNA complexes are usually obtained only at higher polycation/DNA ratios with a strong positive net charge (Kircheis et al 2001). Large particles do not easily enter the cell, and their loose structure is not stable enough to resist the low pH environment and lysosomal enzymes in the cell.…”

Chemistry specificity of DNA–polycation complex salt response: a simulation study of DNA, polylysine and polyethyleneimine

“…1,2 As opposted to viral vectors, polyelectrolyte complexes, polyplexes, offer a infection free gene delivery. On the other hand, gene transfection is a multi-stage process where the design of an optimal carrier is a trade-off of several properties, such as, complex charge, complex stability, and complex size [3][4][5] as the delivery vector has to provide both adequate protection, and efficient and timely release of the gene. 6 In general, PE complexes are known to respond to presence of electrolytes (e.g.…”

Hybrid vector including polyethylenimine and cationic lipid, DOTMA, for gene delivery

“…Polyethylenimine (PEI) is a popular cationic polymer for polyplex and has shown high gene expression in in vitro and in vivo gene delivery studies (Godbey et al, 1999;Kircheis et al, 2001). …”