This paper investigates the potential of PLA/Flax substrates as eco-friendly alternatives for RF (Radio Frequency) antenna applications in the context of sustainability within the electronics industry. As electronic waste continues to mount globally and the use of RF-connected devices proliferates, there is a growing need for sustainable materials. This study explores the dielectric properties of PLA/Flax substrates and their implications for antenna design and performance. Our findings reveal promising RF performance, highlighting the viability of bio-sourced and biodegradable materials in RF engineering to address environmental concerns. Subsequent sections delve into the impact of moisture, and substrate homogeneity, offering valuable insights for sustainable RF systems.