Design and characterization of a chitosan physical gel promoting wound healing in mice

Abstract

In this study, a sterile and biocompatible chitosan (CHI) gel for wound healing applications was formulated. CHI powder was treated in autoclave (ttCHI) to prepare sterile formulations. The heat treatment modified the CHI molecular weight, as evidenced by GPC analysis, and its physical-chemical features. Differential scanning calorimetry studies indicated that the macromolecules, before and after thermal treatment, differ in the strength of water-polymer interaction leading to different viscoelastic and flow properties. Thermally treated CHI exhibited the following effects: (i) increased the proliferation and migration of human foreskin foetal fibroblasts at 24 h; (ii) accelerated wound healing (measured as area of lesion) at 3 and 10 days in an in vivo model of pressure ulcers. These effects were linked to the increase of the hydroxyproline and haemoglobin content as well as Wnt protein expression. Moreover, we found a reduction of myeloperoxidase activity and TNF-α mRNA expression. These observations suggest the potential of this novel CHI gel in wound healing and other therapeutic applications.

Therapeutic effect of low molecular weight chitosan containing sepia ink on ethanol-induced gastric ulcer in rats

“…Collagen is mainly secreted by fibroblasts, which is critical for ulcer healing 20 . Hydroxyproline (Hyp), as a characteristic amino acid of collagen, are often used as an indicator for detecting the collagen content 21 . Present study in Figure 3 showed that the fibroblasts in mucosa lamina of LMCS group secreted more collagen fibers compared to Con group at the testing time.…”

Application of Chitosan and Buriti Oil (Mauritia Flexuosa L.) in Skin Wound Healing

“…So many studies emphasize the combination of chitosan with other materials to improve its effect on healing. Another studies, combined chitosan with other substances and observed acceleration of wound closure and thus, faster healing [32,33,44,45,47,49]. The higher the degree of deacetylation, the greater the efficiency of chitosan in the modulation of human fibroblasts mitogenesis and the greater the cell adhesion and proliferation, both for keratinocytes and fibroblasts, a fact observed in the study by Ueno et al [35] and Chatelet et al [50].…”

“…Many studies have shown that the surface charge of chitosan changes considerably as a function of the pH of the solution in which it is immersed, generating a positive charge that attacks the cell wall of the microorganisms. When the amino groups of chitosan come into contact with the physiological fluids, they are probably protonated and bind to anionic groups of the microorganisms, resulting in agglutination of the microbial cells and inhibition of growth [47,49,52,53].…”

The Use of Biologic Scaffolds in the Treatment of Chronic Nonhealing Wounds

“…86 Control of hemostasis and wound hydration has led to the investigation of chitosan as a wound dressing for nonhealing wounds. [87][88][89][90] The benefit of these scaffolds is their ability to rapidly control hemostasis while also modulating the wound environment to maintain an optimal hydration level and prevent desiccation. Furthermore, these chitosan materials can be combined with other bioactive molecules to provide combination dressings that promote wound healing while also combatting infection 87 or modulating inflammation.…”