Design and Application of New 16S rRNA-targeted Oligonucleotide Probes for the Azospirillum-Skermanella-Rhodocista-Cluster

Stoffels, Marion; Castellanos, Thelma; Hartmann, Anton

doi:10.1078/0723-2020-00011

Cited by 57 publications

(31 citation statements)

References 45 publications

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“…Phylogenetic trees based on 16S rRNA gene sequences have shown that R. centenum is closely related to species of the genus Azospirillum (Stoffels et al, 2001). This casts a new light on the developmental cycle observed with R. centenum since the physiological traits of R. centenum share a number of similarities with Azospirillum spp.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, recent 16S rRNA gene phylogenetic analysis has determined that R. centenum forms a clade with Azospirillum spp. and thus may be considered a photosynthetic-capable Azospirillum-like organism (Stoffels et al, 2001). These similarities led us to examine more carefully the formation of R. centenum cyst cells, to determine how the physiology of the R. centenum resting cysts compares to the well-described physiology of Azotobacter cysts.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of cyst cell formation in the purple photosynthetic bacterium Rhodospirillum centenum

Berleman

Bauer

2004

Microbiology

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Rhodospirillum centenum is an anoxygenic photosynthetic bacterium that is capable of differentiating into several cell types. When grown phototrophically in liquid, cells exhibit a vibrioid shape and have a single polar flagellum. When grown on a solid surface, R. centenum will differentiate into rod-shaped swarm cells that display numerous lateral flagella. Upon starvation for nutrients, R. centenum also forms desiccation-resistant cysts. In this study, it was determined that R. centenum has heat- and desiccation-resistance properties similar to other cyst-forming species. In addition, microscopic analyses of the morphological changes that occur during cyst cell development were performed. It was observed that R. centenum typically forms multi-celled clusters of cysts that contain from four to more than 10 cells per cluster. It was also determined that cell density has a minor effect on the percentage of cyst cells formed, with cell densities of 105–107 cells per 5 μl spot yielding the highest percentage of cyst cells. The striking similarities between the life cycle of R. centenum and the life cycle exhibited by Azospirillum spp. are discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of cyst cell formation in the purple photosynthetic bacterium Rhodospirillum centenum

Berleman

Bauer

2004

Microbiology

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“…These three probes, when combined, detected almost all bacteria. For A. brasilense , the specifi c probe Abras 1420 (Stoffels et al 2001 ) is used. The EUB-338 I, II, and III probes are labeled with the fl uorochrome FITC and the Abras 1420 probe is labeled with the fl uorochrome Cy3.…”

Section: Methodsmentioning

confidence: 99%

Interaction of Azospirillum spp. with Microalgae: A Basic Eukaryotic–Prokaryotic Model and Its Biotechnological Applications

de‐Bashan¹,

Hernández²,

Bashan³

2015

Handbook for Azospirillum

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The interaction of the bacteria Azospirillum spp. with photosynthetic, single cell microalgae that are co-immobilized in alginate beads provides a significant shortcut for understanding the interaction of this plant growth-promoting bacteria (PGPB) with plants in general. This interaction is currently relevant for studying physiological, physical, biochemical, and molecular aspects. As an independent subfi eld of Azospirillum research, this interaction has some signifi cant potential biotechnological applications, such as wastewater treatment, production of biofuel (ethanol and biodiesel), increased fertility of eroded soils combined with promoting growth of higher plants, production of pigments, and production of biomass. All of these applications have yet to be scaled up and evaluated for their true practical potential. The Logic Behind Using This Interaction as a Model for Plant-Bacteria InteractionA major obstacle in the study of interactions between Azospirillum spp. and plants is the complexity of the plant. Studies of basic plant-bacterium interactions of Azospirillum spp., done mainly with roots, are diffi cult because there are manyDedication : This chapter is dedicated to the memory of the German/Spanish mycorrhizae researcher Dr. Horst Vierheilig (1964 of CSIC in Spain.

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“…The use of techniques for detection, identification and quantification of microorganisms based on PCR can be completed by a dependent culture and biochemical methods. The developments of specific species of Azospirillum were studied by Stoffels et al (2001) by analysis of fluorescence in situ hybridization. However, this method can only detect physiologically active bacteria, and can not detect all the members who belong to the genus Azospirillum.…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and molecular characterization of plant growth promoting Rhizobacteria isolated from the rhizosphere of wheat (Triticum durum Desf.) in Algeria

Mahbouba¹,

Christine²,

Belbekri³

et al. 2013

Afr. J. Microbiol. Res.

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Several strains were isolated from rhizosphere of wheat in Eastern Algeria. All the 10 strains (Azo4, Azo5, Azo6, Azo7, Azo8, Azo10, S1, S2, S3 and S9) were identified; based also on the characteristics which are not only morphological, characteristics such as shape but physiological ones: optimal temperature, pH optimum and NaCl tolerance. These isolates were screened in vitro for their growth promoting traits like production of indole acetic acid (IAA), ammonia (NH 3 ) and solubilize phosphate. The bacterial strains have an optimum growth pH equal to 6.8; in a broad temperature range (28 and 37°C). The molecular identification was done by simple PCR to amplification for 16S rDNA gene using primers AZ16S-D. Some strains (Azo4, Azo5, Azo6, Azo7, Azo8 and Azo10) have high identity with genus Azospirillum which indicates that these isolates belong to the Azospirillum genus, specially to species of Azospirillum brasilense A nested PCR approach performed with degenerate primers was used to amplify nifH gene fragments from the bulk DNA. The amplification product bands at the expected nifH gene fragment size are about 370 bp. Some isolates (S1, S2, S3, Azo4, Azo5, Azo6, S9 and Azo10) were considered by the nitrogen-fixing gene nifH detection of their genome. We managed to highlight the contribution between A. brasilense and durum wheat with inoculation experiments performed under greenhouse conditions showed that all A. brasilense (six strains) have a good report for growth of roots and plants and improve the production of durum wheat (Triticum durum var: GTA). These data demonstrate the importance of the test especially in programs to improve the quality of wheat in Algeria.

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Design and Application of New 16S rRNA-targeted Oligonucleotide Probes for the Azospirillum-Skermanella-Rhodocista-Cluster

Cited by 57 publications

References 45 publications

Characterization of cyst cell formation in the purple photosynthetic bacterium Rhodospirillum centenum

Characterization of cyst cell formation in the purple photosynthetic bacterium Rhodospirillum centenum

Interaction of Azospirillum spp. with Microalgae: A Basic Eukaryotic–Prokaryotic Model and Its Biotechnological Applications

Phenotypic and molecular characterization of plant growth promoting Rhizobacteria isolated from the rhizosphere of wheat (Triticum durum Desf.) in Algeria

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