Design and Application of Multi-functional Electrogenerated Chemiluminescence Imaging Analyzer

Jiang, Guangfu; Liu, Xia; Wang, Yaqin; Ruan, Sanpeng; Qi, Honglan; Yang, Yong Quan; Zhou, Qishe; Zhang, Chengxiao

doi:10.2116/analsci.32.1023

Cited by 5 publications

(2 citation statements)

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“…ECL measurements were performed on a multifunctional ECL imaging analyzer (REFP-1) using a photomultiplier tube (PMT) and a charge-coupled device (CCD) as detectors . Electrochemical measurement was performed on CHI 660E.…”

Section: Methodsmentioning

confidence: 99%

“…Bis(2,2′-bipyridine)-4′-methyl-4-carboxybipyridine-ruthenium N-succinimidyl ester-bis-(hexafluorophosphate) (the cation was abbreviated as Ru1), bovine serum albumin (BSA) were purchased from Sigma-Aldrich (USA ECL measurements were performed on a multifunctional ECL imaging analyzer (REFP-1) using a photomultiplier tube (PMT) and a charge-coupled device (CCD) as detectors. 26 Electrochemical measurement was performed on CHI 660E. UV−vis absorption spectrophotometer (UV-2450, Shimadzu, Japan), scanning electron microscope (SEM, TM3030, Hitachi, Japan), field-emission scanning electron microscope (FE-SEM, SU-8820, Hitachi, Japan), OCA 20 video optical contact angle measuring instrument (Dataphysics, Germany), thermostatic vortex mixer (MTC-100, Hangzhou Miu Instruments Co. Ltd., China), and 12-hole dual-purpose magnetic shelf (GO-MRTD-12S, GeneOn BioTech Co., Ltd. China) were employed in this work.…”

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confidence: 99%

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Electrogenerated Chemiluminescence Immunoassays on Nanoelectrode Ensembles Platform with Magnetic Microbeads for the Determination of Carbohydrate Antigen

Yang

Wei

et al. 2020

Anal. Chem.

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This work reports a gold nanoelectrode ensembles (Au-NEE) platform taken as a disposable electrogenerated chemiluminescence (ECL) platform with immunomagnetic microbeads for ECL immunoassays for the first time. The peak-shaped voltammograms were obtained at the Au-NEE, attributed to the total diffusional overlap. The ECL intensity at Au-NEE was 12.9 folds in the Ru(bpy) 3 2+ -tri-n-propylamine (TPA) ECL system and 19.6 folds in the luminol-H 2 O 2 system, compared with that at the Au macroelectrode using the normalized active area of the electrodes, mainly attributed to the diffusion overlap of the Au-NEE and the edge effect of the individual gold nanodisks of the Au-NEE. The ECL immunoassay on the Au-NEE platform with magnetic microbeads for the determination of cancer biomarkers was developed. Carbohydrate antigen 19-9 (CA 19-9) was chosen as a model analyte while CA 19-9 antibody on the magnetic microbeads was taken as the capture probe, and ruthenium complex-labeled CA 19-9 antibody was used as the signal probe. A "sandwich" bioconjugates on the magnetic beads were transferred onto the ECL platform, and then the ECL measurements were performed in TPA solution. The developed method showed that the ECL peak intensity was directly in proportion to the concentration of CA 19-9 in the range from 0.5 to 20 U/mL with a limit of detection of 0.4 U/mL. This work demonstrates that the Au-NEE can be employed as a useful disposable ECL platform with the merits of cheapness, low nonspecific adsorption and practical application. The proposed approach will open a new avenue in the point-of-care test for the determination of protein biomarkers.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%