Desiccation Tolerance: Gene Expression, Pathways, and Regulation of Gene Expression

Bartels, Dorothea; Phillips, Jonathan; Chandler, John

doi:10.1002/9780470376881.ch5

Cited by 10 publications

(5 citation statements)

References 185 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Early changes in cell turgor, cell wall tension, and solute concentration are thought to be involved (Bartels et al , 2007). If this were true, a fast decrease of RWC upon RD could lead to an early de-epoxidation of V to A and then to Z.…”

Section: Discussionmentioning

confidence: 99%

Evidence for the absence of enzymatic reactions in the glassy state. A case study of xanthophyll cycle pigments in the desiccation-tolerant moss Syntrichia ruralis

Fernández‐Marín

Kranner

Sebastián

et al. 2013

Journal of Experimental Botany

View full text Add to dashboard Cite

Desiccation-tolerant plants are able to withstand dehydration and resume normal metabolic functions upon rehydration. These plants can be dehydrated until their cytoplasm enters a ‘glassy state’ in which molecular mobility is severely reduced. In desiccation-tolerant seeds, longevity can be enhanced by drying and lowering storage temperature. In these conditions, they still deteriorate slowly, but it is not known if deteriorative processes include enzyme activity. The storage stability of photosynthetic organisms is less studied, and no reports are available on the glassy state in photosynthetic tissues. Here, the desiccation-tolerant moss Syntrichia ruralis was dehydrated at either 75% or <5% relative humidity, resulting in slow (SD) or rapid desiccation (RD), respectively, and different residual water content of the desiccated tissues. The molecular mobility within dry mosses was assessed through dynamic mechanical thermal analysis, showing that at room temperature only rapidly desiccated samples entered the glassy state, whereas slowly desiccated samples were in a ‘rubbery’ state. Violaxanthin cycle activity, accumulation of plastoglobules, and reorganization of thylakoids were observed upon SD, but not upon RD. Violaxanthin cycle activity critically depends on the activity of violaxanthin de-epoxidase (VDE). Hence, it is proposed that enzymatic activity occurred in the rubbery state (after SD), and that in the glassy state (after RD) no VDE activity was possible. Furthermore, evidence is provided that zeaxanthin has some role in recovery apparently independent of its role in non-photochemical quenching of chlorophyll fluorescence.

show abstract

Section: Discussionmentioning

confidence: 99%

Evidence for the absence of enzymatic reactions in the glassy state. A case study of xanthophyll cycle pigments in the desiccation-tolerant moss Syntrichia ruralis

Fernández‐Marín

Kranner

Sebastián

et al. 2013

Journal of Experimental Botany

View full text Add to dashboard Cite

show abstract

“…The CDT-1 transcript of the resurrection plant C. plantagineum is induced specifically by dehydration in vegetative organs and also by abscisic acid (ABA) (Furini et al 1997). Desiccation sensitive C. plantagineum calli becomes desiccation tolerant and transcribes dehydration-related genes when transformed with CDT-1 ( Bartels et al 2007). CDT-1 was shown to be a retroelement that produces a non-coding RNA that directs the synthesis of a double-stranded 21-bp short interfering RNA (siRNA) (Hilbricht et al 2008).…”

Section: Proteins Involved In Gene Regulationmentioning

confidence: 99%

Proteomic analysis of nuclear proteins during dehydration of the resurrection plant Xerophyta viscosa

2010

View full text Add to dashboard Cite

Xerophyta viscosa (family Velloziaceae) is a monocotyledonous poikilochlorophyllous desiccation tolerant plant capable of surviving drying its leaf tissue to 5% relative water content (RWC) for prolonged periods and resuming full physiological activity upon rehydration. X. viscosa and other resurrection plants are excellent model systems for the study of desiccation tolerance mechanisms. In this work, the X. viscosa nuclear protein profile was investigated in response to dehydration stress using 2D gel electrophoresis, protein map analysis and mass spectrometry identification. From an average of 438 protein spots detected on the gels, 18 spots were significantly shown to increase in abundance upon dehydration at 35% RWC. Analysis of the 18 dehydration-induced nuclear protein spots resulted in the identification of proteins associated with gene transcription and regulation, cell signalling, molecular chaperone and proteolysis type activities, protein translation, energy metabolism, and novel proteins. This study revealed that X. viscosa nucleus responded to dehydration stress and that desiccation tolerance was controlled by multiple genes within the plant nucleus. This study indicated that adjusting gene regulation and expression of appropriate stress response proteins in the nucleus was sufficient to protect the cellular structures during dehydration and in the dried state.

show abstract

“…have long attracted the attention of botanists, and, more recently, have been subjected to a detailed dissection of the physiological, metabolic and molecular basis of their tolerance (e.g. Bartels et al 2007;Moore et al 2009). In several resurrection plants, ABA was found to be involved with desiccation tolerance by inducing the accumulation of stress-protective proteins (e.g.…”

Section: Desiccation Tolerance As a Metabolic Phenomenonmentioning

confidence: 99%

Towards a conceptual ABA ideotype in plant breeding for water limited environments

Blum¹

2015

Functional Plant Biol.

View full text Add to dashboard Cite

A huge amount of information had been accumulated on abscisic acid (ABA). Laboratory and some field research with ABA-enhanced transgenic plants generally conclude that ABA is a drought resistance hormone, since it causes stomatal closure, reduces transpiration and results in ‘water saving’ under drought stress. This recurring conclusion is hard to accept in the agronomic domain considering the many direct and indirect negative effects of ABA on plant growth and reproduction. In order to formulate a conceptual phenotypic ABA ideotype for plant breeding, this paper begins by briefly reviewing the phenomics of ABA relative to plant function and productivity. Consequently, it is recognised that ABA enhancement is important in controlling the isohydric (‘water saving’) plant model, whereas plant hydraulics are more important in controlling the anisohydric (‘water spending’) plant model. Subsequently, the respective isohydric and anisohydric ideotypes appropriate to specific dryland crop drought stress scenarios are proposed. It is concluded that ABA can by no means be universally defined as a ‘drought resistance hormone’. Its benefit or damage depends on the crop drought stress profile and the dynamics of the seasonal regimen of ABA in the plant. The isohydric ideotype might have an advantage in the harshest environments, whereas the anisohydric one will perform relatively better under more moderate drought conditions.

show abstract

Desiccation Tolerance: Gene Expression, Pathways, and Regulation of Gene Expression

Cited by 10 publications

References 185 publications

Evidence for the absence of enzymatic reactions in the glassy state. A case study of xanthophyll cycle pigments in the desiccation-tolerant moss Syntrichia ruralis

Evidence for the absence of enzymatic reactions in the glassy state. A case study of xanthophyll cycle pigments in the desiccation-tolerant moss Syntrichia ruralis

Proteomic analysis of nuclear proteins during dehydration of the resurrection plant Xerophyta viscosa

Towards a conceptual ABA ideotype in plant breeding for water limited environments

Contact Info

Product

Resources

About